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Product Info Summary
| SKU: | A00699-2 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-PKA I alpha reg PRKAR1A Antibody
View all Protein Kinase A regulatory subunit I alpha Antibodies
SKU/Catalog Number
A00699-2
Size
100μl
Form
Liquid
Description
Boster Bio Anti-PKA I alpha reg PRKAR1A Antibody catalog # A00699-2. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PKA I alpha reg PRKAR1A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00699-2)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human KAP0. AA range:271-320
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00699-2 is reactive to PRKAR1A in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
42982 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00699-2 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of 293 cells using PKA Ialpha reg Polyclonal antibody.
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Western Blot (WB) analysis of specific cells using PKA Ialpha reg Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For PRKAR1A (Source: Uniprot.org, NCBI)
Gene Name
PRKAR1A
Full Name
cAMP-dependent protein kinase type I-alpha regulatory subunit
Weight
42982 MW
Superfamily
cAMP-dependent kinase regulatory chain family
Alternative Names
cAMP-dependent protein kinase type I-alpha regulatory subunit;Tissue-specific extinguisher 1;TSE1;cAMP-dependent protein kinase type I-alpha regulatory subunit, N-terminally processed;PRKAR1A;PKR1, PRKAR1, TSE1; PRKAR1A ACRDYS1, ADOHR, CAR, CNC, CNC1, PKR1, PPNAD1, PRKAR1, TSE1 protein kinase cAMP-dependent type I regulatory subunit alpha cAMP-dependent protein kinase type I-alpha regulatory subunit|Carney complex type 1|cAMP-dependent protein kinase regulatory subunit RIalpha|cAMP-dependent protein kinase type I-alpha regulatory chain|epididymis secretory sperm binding protein|protein kinase A type 1a regulatory subunit|protein kinase, cAMP-dependent, regulatory subunit type I alpha|protein kinase, cAMP-dependent, regulatory, type I, alpha|tissue-specific extinguisher 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRKAR1A, check out the PRKAR1A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRKAR1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PKA I alpha reg PRKAR1A Antibody (A00699-2)
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No publications found for A00699-2
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-PKA I alpha reg PRKAR1A Antibody
Question
My question regarding product A00699-2, anti-PKA I alpha reg antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00699-2 anti-PKA I alpha reg antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-20
Question
See below the WB image, lot number and protocol we used for platelet using anti-PKA I alpha reg antibody A00699-2. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-18
Question
Will anti-PKA I alpha reg antibody A00699-2 work for WB with platelet?
K. Singh
Verified customer
Asked: 2017-11-29
Answer
According to the expression profile of platelet, PRKAR1A is highly expressed in platelet. So, it is likely that anti-PKA I alpha reg antibody A00699-2 will work for WB with platelet.
Boster Scientific Support
Answered: 2017-11-29
Question
Is a blocking peptide available for product anti-PKA I alpha reg antibody (A00699-2)?
Verified Customer
Verified customer
Asked: 2017-08-11
Answer
We do provide the blocking peptide for product anti-PKA I alpha reg antibody (A00699-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-08-11
Question
Would A00699-2 anti-PKA I alpha reg antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-07-10
Answer
It shows on the product datasheet, A00699-2 anti-PKA I alpha reg antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-07-10
Question
I see that the anti-PKA I alpha reg antibody A00699-2 works with WB, what is the protocol used to produce the result images on the product page?
Z. Huang
Verified customer
Asked: 2015-03-27
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2015-03-27
