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Product Info Summary
| SKU: | A00699-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Protein Kinase A regulatory subunit I alpha/PRKAR1A Antibody Picoband®
View all Protein Kinase A regulatory subunit I alpha Antibodies
SKU/Catalog Number
A00699-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Protein Kinase A regulatory subunit I alpha/PRKAR1A Antibody Picoband® catalog # A00699-1. Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Protein Kinase A regulatory subunit I alpha/PRKAR1A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00699-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PRKAR1A recombinant protein (Position: E2-E81). Human PRKAR1A shares 89.9% and 92.4% amino acid (aa) sequence identity with mouse and rat PRKAR1A, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A00699-1 is reactive to PRKAR1A in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
48 kDa
Calculated molecular weight
42982 MW
Background of Protein Kinase A regulatory subunit I alpha
cAMP-dependent protein kinase type I-alpha regulatory subunit is an enzyme that in humans is encoded by the PRKAR1A gene. This protein encoded by this gene was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids. Mutations in this gene cause Carney complex (CNC). This gene can fuse to the RET protooncogene by gene rearrangement and form the thyroid tumor-specific chimeric oncogene known as PTC2. A nonconventional nuclear localization sequence (NLS) has been found for this protein which suggests a role in DNA replication via the protein serving as a nuclear transport protein for the second subunit of the Replication Factor C (RFC40). Several alternatively spliced transcript variants encoding two different isoforms have been observed.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00699-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: rat thymus tissue, human HepG2 whole cell, human MCF-7 whole cell
IHC: rat testis tissue, human intestinal cancer tissue, human lung cancer tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PRKAR1A using anti-PRKAR1A antibody (A00699-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat thymus tissue lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRKAR1A antigen affinity purified polyclonal antibody (Catalog # A00699-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRKAR1A at approximately 48 kDa. The expected band size for PRKAR1A is at 43KD.
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Figure 2. IHC analysis of PRKAR1A using anti-PRKAR1A antibody (A00699-1).
PRKAR1A was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-PRKAR1A Antibody (A00699-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IHC analysis of PRKAR1A using anti-PRKAR1A antibody (A00699-1).
PRKAR1A was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-PRKAR1A Antibody (A00699-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 4. IHC analysis of PRKAR1A using anti-PRKAR1A antibody (A00699-1).
PRKAR1A was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-PRKAR1A Antibody (A00699-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For PRKAR1A (Source: Uniprot.org, NCBI)
Gene Name
PRKAR1A
Full Name
cAMP-dependent protein kinase type I-alpha regulatory subunit
Weight
42982 MW
Superfamily
cAMP-dependent kinase regulatory chain family
Alternative Names
cAMP-dependent protein kinase type I-alpha regulatory subunit;Tissue-specific extinguisher 1;TSE1;cAMP-dependent protein kinase type I-alpha regulatory subunit, N-terminally processed;PRKAR1A;PKR1, PRKAR1, TSE1; PRKAR1A ACRDYS1, ADOHR, CAR, CNC, CNC1, PKR1, PPNAD1, PRKAR1, TSE1 protein kinase cAMP-dependent type I regulatory subunit alpha cAMP-dependent protein kinase type I-alpha regulatory subunit|Carney complex type 1|cAMP-dependent protein kinase regulatory subunit RIalpha|cAMP-dependent protein kinase type I-alpha regulatory chain|epididymis secretory sperm binding protein|protein kinase A type 1a regulatory subunit|protein kinase, cAMP-dependent, regulatory subunit type I alpha|protein kinase, cAMP-dependent, regulatory, type I, alpha|tissue-specific extinguisher 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRKAR1A, check out the PRKAR1A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRKAR1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Protein Kinase A regulatory subunit I alpha/PRKAR1A Antibody Picoband® (A00699-1)
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Customer Q&As
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2 Customer Q&As for Anti-Protein Kinase A regulatory subunit I alpha/PRKAR1A Antibody Picoband®
Question
We are currently using anti-Protein Kinase A regulatory subunit I alpha/PRKAR1A antibody A00699-1 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, rat. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-12
Answer
The anti-Protein Kinase A regulatory subunit I alpha/PRKAR1A antibody (A00699-1) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-12
Question
Is this A00699-1 anti-Protein Kinase A regulatory subunit I alpha/PRKAR1A antibody reactive to the isotypes of PRKAR1A?
Verified Customer
Verified customer
Asked: 2020-01-27
Answer
The immunogen of A00699-1 anti-Protein Kinase A regulatory subunit I alpha/PRKAR1A antibody is E.coli-derived human PRKAR1A recombinant protein (Position: E2-E81). Human PRKAR1A shares 89.9% and 92.4% amino acid (aa) sequence identity with mouse and rat PRKAR1A, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-27
