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Product Info Summary
| SKU: | A03447-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-SLC31A1/CTR1 Antibody Picoband®
View all SLC31A1/CTR1 Antibodies
SKU/Catalog Number
A03447-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SLC31A1/CTR1 Antibody Picoband® catalog # A03447-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SLC31A1/CTR1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03447-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human SLC31A1/CTR1, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03447-1 is reactive to SLC31A1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
26 kDa
Calculated molecular weight
31203 MW
Background of SLC31A1/CTR1
High affinity copper uptake protein 1 (Ctr1) is a protein that in humans is encoded by the SLC31A1 gene. This gene is maped to 9q32. The protein encoded by this gene is a high-affinity copper transporter found in the cell membrane. The encoded protein functions as a homotrimer to effect the uptake of dietary copper.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03447-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Positive Control
WB: human HepG2 whole cell, human PANC-1 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SLC31A1/CTR1 using anti-SLC31A1/CTR1 antibody (A03447-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human PANC-1 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC31A1/CTR1 antigen affinity purified polyclonal antibody (Catalog # A03447-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC31A1/CTR1 at approximately 26KD. The expected band size for SLC31A1/CTR1 is at 21KD.
Protein Target Info & Infographic
Gene/Protein Information For SLC31A1 (Source: Uniprot.org, NCBI)
Gene Name
SLC31A1
Full Name
High affinity copper uptake protein 1
Weight
31203 MW
Superfamily
copper transporter (Ctr) (TC 1.A.56) family
Alternative Names
High affinity copper uptake protein 1; Copper transporter 1; hCTR1; Solute carrier family 31 member 1; SLC31A1; COPT1; CTR1 SLC31A1 COPT1, CTR1 solute carrier family 31 member 1 high affinity copper uptake protein 1|copper transport 1 homolog|copper transporter 1|solute carrier family 31 (copper transporter), member 1|solute carrier family 31 (copper transporters), member 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SLC31A1, check out the SLC31A1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SLC31A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SLC31A1/CTR1 Antibody Picoband® (A03447-1)
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Customer Q&As
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6 Customer Q&As for Anti-SLC31A1/CTR1 Antibody Picoband®
Question
We are currently using anti-SLC31A1/CTR1 antibody A03447-1 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
The anti-SLC31A1/CTR1 antibody (A03447-1) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-22
Question
Would anti-SLC31A1/CTR1 antibody A03447-1 work on primate WB with testis?
Verified Customer
Verified customer
Asked: 2019-11-27
Answer
Our lab technicians have not tested anti-SLC31A1/CTR1 antibody A03447-1 on primate. You can run a BLAST between primate and the immunogen sequence of anti-SLC31A1/CTR1 antibody A03447-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate testis in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-27
Question
I was wanting to use your anti-SLC31A1/CTR1 antibody for WB for human saliva-secreting gland on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human saliva-secreting gland identification?
Verified Customer
Verified customer
Asked: 2019-11-13
Answer
It shows on the product datasheet, A03447-1 anti-SLC31A1/CTR1 antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human saliva-secreting gland in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-13
Question
My question regarding product A03447-1, anti-SLC31A1/CTR1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-02-09
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03447-1 anti-SLC31A1/CTR1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-02-09
Question
I am interested in to test anti-SLC31A1/CTR1 antibody A03447-1 on human saliva-secreting gland for research purposes, then I may be interested in using anti-SLC31A1/CTR1 antibody A03447-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-07-19
Answer
The products we sell, including anti-SLC31A1/CTR1 antibody A03447-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-07-19
Question
Would A03447-1 anti-SLC31A1/CTR1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
T. Patel
Verified customer
Asked: 2014-11-07
Answer
It shows on the product datasheet, A03447-1 anti-SLC31A1/CTR1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-11-07
