Anti-CARD12/NLRC4 Antibody Picoband®

CARD12 antibody

Boster Bio Anti-CARD12/NLRC4 Antibody Picoband® catalog # PB9673. Tested in IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: PB9673
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IF, ICC, WB

Product Name

Anti-CARD12/NLRC4 Antibody Picoband®

View all CARD12 Antibodies

SKU/Catalog Number

PB9673

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CARD12/NLRC4 Antibody Picoband® catalog # PB9673. Tested in IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CARD12/NLRC4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9673)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human CARD12, different from the related mouse sequence by five amino acids, and from the related rat sequence by seven amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9673 is reactive to NLRC4 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

116 kDa

Calculated molecular weight

116159 MW

Background of CARD12

NLR family CARD domain-containing protein 4, also known as CARD12, is a protein that in humans is encoded by the NLRC4 gene. It is mapped to 2p22.3. This gene encodes a member of the caspase recruitment domain-containing NLR family. Family members play essential roles in innate immune response to a wide range of pathogenic organisms, tissue damage and other cellular stresses. Mutations in this gene result in autoinflammation with infantile enterocolitis. Alternative splicing results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PB9673 is guaranteed for IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human

Positive Control

WB: 22RV1 Whole Cell, RH35 Whole Cell, NIH3T3 Whole Cell

Validation Images & Assay Conditions

Gene/Protein Information For NLRC4 (Source: Uniprot.org, NCBI)

Gene Name

NLRC4

Full Name

NLR family CARD domain-containing protein 4

Weight

116159 MW

Alternative Names

NLR family CARD domain-containing protein 4;CARD, LRR, and NACHT-containing protein;Clan protein;Caspase recruitment domain-containing protein 12;Ice protease-activating factor;Ipaf;NLRC4;CARD12, CLAN, CLAN1, IPAF;UNQ6189/PRO20215; NLRC4 AIFEC, CARD12, CLAN, CLAN1, CLANA, CLANB, CLANC, CLAND, CLR2.1, FCAS4, IPAF NLR family CARD domain containing 4 NLR family CARD domain-containing protein 4|CARD, LRR, and NACHT-containing protein|ICE-protease activating factor|NOD-like receptor C4|caspase recruitment domain family, member 12|caspase recruitment domain-containing protein 12|nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 4

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on NLRC4, check out the NLRC4 Infographic

NLRC4 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for NLRC4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-CARD12/NLRC4 Antibody Picoband®

Question

We need to test anti-CARD12/NLRC4 antibody PB9673 on rat lung testis for research purposes, then I may be interested in using anti-CARD12/NLRC4 antibody PB9673 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-04-16

Answer

The products we sell, including anti-CARD12/NLRC4 antibody PB9673, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-04-16

Question

I was wanting to use your anti-CARD12/NLRC4 antibody for WB for rat lung testis on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat lung testis identification?

J. Parker

Verified customer

Asked: 2019-10-18

Answer

You can see on the product datasheet, PB9673 anti-CARD12/NLRC4 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat lung testis in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-10-18

Question

We are currently using anti-CARD12/NLRC4 antibody PB9673 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2019-08-08

Answer

The anti-CARD12/NLRC4 antibody (PB9673) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-08-08

Question

Is a blocking peptide available for product anti-CARD12/NLRC4 antibody (PB9673)?

Verified Customer

Verified customer

Asked: 2019-04-19

Answer

We do provide the blocking peptide for product anti-CARD12/NLRC4 antibody (PB9673). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-04-19

Question

I have a question about product PB9673, anti-CARD12/NLRC4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

J. Banerjee

Verified customer

Asked: 2014-05-12

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9673 anti-CARD12/NLRC4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2014-05-12

Order DetailsPrice
PB9673

100μg

$370
PB9673-10ug

10μg sample (liquid)

$99
PB9673-Biotin

100 μg Biotin conjugated

$570
PB9673-Cy3

100 μg Cy3 conjugated

$570
PB9673-Dylight488

100 μg Dylight488 conjugated

$570
PB9673-Dylight550

100 μg Dylight550 conjugated

$570
PB9673-Dylight594

100 μg Dylight594 conjugated

$570
PB9673-FITC

100 μg FITC conjugated

$570
PB9673-HRP

100 μg HRP conjugated

$570
PB9673-APC

100 μg APC conjugated

$670
PB9673-PE

100 μg PE conjugated

$670
PB9673-iFluor647

100 μg iFluor647 conjugated

$670
PB9673-carrier-free

Carrier Free

$370

More conjugates/formats

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PB9673
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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