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Product Info Summary
| SKU: | PB9673 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-CARD12/NLRC4 Antibody Picoband®
SKU/Catalog Number
PB9673
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CARD12/NLRC4 Antibody Picoband® catalog # PB9673. Tested in IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CARD12/NLRC4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9673)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human CARD12, different from the related mouse sequence by five amino acids, and from the related rat sequence by seven amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9673 is reactive to NLRC4 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
116 kDa
Calculated molecular weight
116159 MW
Background of CARD12
NLR family CARD domain-containing protein 4, also known as CARD12, is a protein that in humans is encoded by the NLRC4 gene. It is mapped to 2p22.3. This gene encodes a member of the caspase recruitment domain-containing NLR family. Family members play essential roles in innate immune response to a wide range of pathogenic organisms, tissue damage and other cellular stresses. Mutations in this gene result in autoinflammation with infantile enterocolitis. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9673 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Positive Control
WB: 22RV1 Whole Cell, RH35 Whole Cell, NIH3T3 Whole Cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CARD12 using anti-CARD12 antibody (PB9673).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions.
Lane 1: 22RV1 Whole Cell Lysate,
Lane 2: RH35 Whole Cell Lysate,
Lane 3: NIH3T3 Whole Cell Lysate.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CARD12 antigen affinity purified polyclonal antibody (Catalog # PB9673) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CARD12 at approximately 116 kDa. The expected band size for CARD12 is at 116 kDa.
Protein Target Info & Infographic
Gene/Protein Information For NLRC4 (Source: Uniprot.org, NCBI)
Gene Name
NLRC4
Full Name
NLR family CARD domain-containing protein 4
Weight
116159 MW
Alternative Names
NLR family CARD domain-containing protein 4;CARD, LRR, and NACHT-containing protein;Clan protein;Caspase recruitment domain-containing protein 12;Ice protease-activating factor;Ipaf;NLRC4;CARD12, CLAN, CLAN1, IPAF;UNQ6189/PRO20215; NLRC4 AIFEC, CARD12, CLAN, CLAN1, CLANA, CLANB, CLANC, CLAND, CLR2.1, FCAS4, IPAF NLR family CARD domain containing 4 NLR family CARD domain-containing protein 4|CARD, LRR, and NACHT-containing protein|ICE-protease activating factor|NOD-like receptor C4|caspase recruitment domain family, member 12|caspase recruitment domain-containing protein 12|nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NLRC4, check out the NLRC4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NLRC4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CARD12/NLRC4 Antibody Picoband® (PB9673)
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Customer Q&As
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5 Customer Q&As for Anti-CARD12/NLRC4 Antibody Picoband®
Question
We need to test anti-CARD12/NLRC4 antibody PB9673 on rat lung testis for research purposes, then I may be interested in using anti-CARD12/NLRC4 antibody PB9673 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-04-16
Answer
The products we sell, including anti-CARD12/NLRC4 antibody PB9673, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-04-16
Question
I was wanting to use your anti-CARD12/NLRC4 antibody for WB for rat lung testis on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat lung testis identification?
J. Parker
Verified customer
Asked: 2019-10-18
Answer
You can see on the product datasheet, PB9673 anti-CARD12/NLRC4 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat lung testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-18
Question
We are currently using anti-CARD12/NLRC4 antibody PB9673 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-08
Answer
The anti-CARD12/NLRC4 antibody (PB9673) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-08
Question
Is a blocking peptide available for product anti-CARD12/NLRC4 antibody (PB9673)?
Verified Customer
Verified customer
Asked: 2019-04-19
Answer
We do provide the blocking peptide for product anti-CARD12/NLRC4 antibody (PB9673). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-04-19
Question
I have a question about product PB9673, anti-CARD12/NLRC4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Banerjee
Verified customer
Asked: 2014-05-12
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9673 anti-CARD12/NLRC4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-05-12
