Anti-Ipaf NLRC4 Antibody

Product Name

Anti-Ipaf NLRC4 Antibody

View all CARD12 Antibodies

SKU/Catalog Number

A00525

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Ipaf NLRC4 Antibody (Catalog # A00525). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human.

Storage & Handling

Ipaf antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Ipaf NLRC4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00525)

Host

Rabbit

Contents

Ipaf Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Ipaf antibody was raised against a synthetic peptide corresponding to amino acids near the C-terminus of human Ipaf. The immunogen is located within the last 50 amino acids of Ipaf.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least two isoforms of KEAP1 are known to exist.

Reactive Species

A00525 is reactive to NLRC4 in Human

Applications

A00525 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

116159 MW

Background of CARD12

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adaptor molecules and proteases including several members of the caspase family. Another family of proteins that functions as a critical regulator of apoptosis and NFκ signaling pathways is the CED-4/Apaf-1 (apoptosis protein activating factor-1) protein family. Ipaf (ICE protease activating factor) is a CED-4/Apaf-1 family member that activates caspase-1/ICE and can induce apoptosis in human cells in a caspase-1 dependent manner. Ipaf and caspase-1 are thought to interact with each other through the association of the Ipaf amino-terminal CARD (caspase recruitment domain) and amino-terminal CARD of caspase-1.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Ipaf polyclonal antibody can be used for the detection of Ipaf by Western blot at 1 and 2 μg/mL. A 110 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of Ipaf in human PBL lysate with Ipaf antibody at 0.5 (lane A)

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Immunocytochemistry of Ipaf in THP-1 cells with Ipaf antibody at 10 μg/mL.

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Immunofluorescence of Ipaf in THP1 cells with Ipaf antibody at 10 μg/mL.

Gene/Protein Information For NLRC4 (Source: Uniprot.org, NCBI)

Alternative Names

CARD12leucine rich repeat and CARD domaincontaining 4; caspase recruitment domain family, member 12; Caspase recruitment domain-containing protein 12; Clan protein; CLAN1ipaf; CLANCARD, LRR, and NACHT-containing protein; CLR2.1; Ice protease-activating factor; Ipaf; NLR family, CARD domain containing 4 NLRC4 AIFEC, CARD12, CLAN, CLAN1, CLANA, CLANB, CLANC, CLAND, CLR2.1, FCAS4, IPAF NLR family CARD domain containing 4 NLR family CARD domain-containing protein 4|CARD, LRR, and NACHT-containing protein|ICE-protease activating factor|NOD-like receptor C4|caspase recruitment domain family, member 12|caspase recruitment domain-containing protein 12|nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 4

For more info on NLRC4, check out the NLRC4 Infographic

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In this infographic, you will see the following information for NLRC4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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