Anti-PDL2 PDCD1LG2 Monoclonal Antibody [4E10]

Product Name

Anti-PDL2 PDCD1LG2 Monoclonal Antibody [4E10]

View all PD-L2/B7-DC/PDCD1LG2 Antibodies

SKU/Catalog Number

M03295

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-PDL2 PDCD1LG2 Monoclonal Antibody [4E10] (Catalog # M03295). Tested in ELISA, WB, IHC-P, ICC, IF, Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

PD-L2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-PDL2 PDCD1LG2 Monoclonal Antibody [4E10] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03295)

Host

Mouse

Contents

PD-L2 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.

Clonality

Monoclonal

Clone Number

Clone: 4E10

Isotype

IgG1

Immunogen

PD-L2 antibody was raised against the extracellular domain of human PD-L2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

beta-Actin antibody is human, mouse, rat, rabbit, chicken, zebrafish and drosophila reactive.

Reactive Species

M03295 is reactive to PDCD1LG2 in Human

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

57449 MW

Background of PD-L2/B7-DC/PDCD1LG2

Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by co gnate peptides bound to MHC molecules on antigen-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PD-L1 and PD-L2, both of which are thought act as a negative regulator of T cell activation. However, it has been suggested that PD-L2 can act to stimulate an immunogenic response through and alternative receptor from PD-1.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

M03295 is guaranteed for ELISA, Flow Cytometry, IF, IHC-P, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

PD-L2 antibody can be used for detection of PD-L2 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For PDCD1LG2 (Source: Uniprot.org, NCBI)

Alternative Names

B7DC, Btdc, PDL2, CD273, PD-L2, PDCD1L2, bA574F11.2, B7DC, Programmed cell death 1 ligand 2, Butyrophilin B7-DC, PD-1 ligand 2 Pdcd1lg2|B7-DC, Btdc, F730015O22Rik, PD-, PD-L2|programmed cell death 1 ligand 2|programmed cell death 1 ligand 2|PD-1 ligand 2|PDCD1 ligand 2|butyrophilin B7-DC|butyrophilin-like protein|programmed death ligand 2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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