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Product Info Summary
| SKU: | A03295 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-PD-L2 PDCD1LG2 Antibody
View all PD-L2/B7-DC/PDCD1LG2 Antibodies
SKU/Catalog Number
A03295
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-PD-L2 PDCD1LG2 Antibody (Catalog # A03295). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
PD-L2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-PD-L2 PDCD1LG2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03295)
Host
Rabbit
Contents
PD-L2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-PD-L2 antibody was raised against a peptide corresponding to 16 amino acids near the center of human PD-L2. The immunogen is located within amino acids 140-190 of PD-L2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody detects all isoforms of IL-32.
Reactive Species
A03295 is reactive to PDCD1LG2 in Human, Mouse
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
30957 MW
Background of PD-L2/B7-DC/PDCD1LG2
Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by co gnate peptides bound to MHC molecules on antigen-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PD-L1 and PD-L2, both of which are thought act as a negative regulator of T cell activation. However, it has been suggested that PD-L2 can act to stimulate an immunogenic response through and alternative receptor from PD-1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03295 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 0.5-4 μg/mL; IHC: 2.5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in mouse samples; Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western Blot Validation in Human Raji Cell Lysate
Loading: 15 μg of lysates per lane.Antibodies: PD-L2 A03295 (A: 0.5 μg/mL and B: 1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Independent Antibody Validation (IAV) via Protein Expression Profile in Human and Mouse Cell Lines
Loading: 15 μg of lysates per lane.Antibodies: PD-L2, A03295 (4 μg/mL), competitor antibody (4 μg/mL), and beta-actin (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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KO Validation in HeLa Cells
Loading: 15 μg of HeLa WT cell lysates or PD-L2 KO cell lysates. Antibodies: PD-L2, A03295 (4 μg/mL) and beta-actin (1 μg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
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KO Validation in HeLa Cells
Loading: 15 μg of HeLa WT cell lysates or PD-L2 KO cell lysates. Antibodies: PD-L2
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Immunohistochemistry Validation of PD-L2 in Mouse Brain Tissue
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PD-L2 antibody (A03295) at 2.5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunofluorescence Validation of PD-L2 in Mouse Brain Tissue
Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain cells labeling PD-L2 with A03295 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
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Immunohistochemistry Validation of PD-L2 in Mouse Brain Tissue
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PD-L2 antibody (A03295) at 2.5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunofluorescence Validation of PD-L2 in Mouse Brain Tissue
Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain tissue labeling PD-L2 with A03295 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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Immunohistochemistry Validation of PD-L2 in Lung Tumor of Mice (Kao et al.
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Regulated Expression Validation of PD-L2 in Mice with Melanoma Tumor (Knox et al.
Protein Target Info & Infographic
Gene/Protein Information For PDCD1LG2 (Source: Uniprot.org, NCBI)
Gene Name
PDCD1LG2
Full Name
Programmed cell death 1 ligand 2
Weight
30957 MW
Superfamily
immunoglobulin superfamily
Alternative Names
B7DC, Btdc, PDL2, CD273, PD-L2, PDCD1L2, bA574F11.2, B7DC, Programmed cell death 1 ligand 2, Butyrophilin B7-DC, PD-1 ligand 2 Pdcd1lg2|B7-DC, Btdc, F730015O22Rik, PD-, PD-L2|programmed cell death 1 ligand 2|programmed cell death 1 ligand 2|PD-1 ligand 2|PDCD1 ligand 2|butyrophilin B7-DC|butyrophilin-like protein|programmed death ligand 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PDCD1LG2, check out the PDCD1LG2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PDCD1LG2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PD-L2 PDCD1LG2 Antibody (A03295)
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Customer Q&As
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1 Customer Q&As for Anti-PD-L2 PDCD1LG2 Antibody
Question
Is 100ug enough for 100 patients for use in IHC-P?
Verified customer
Asked: 2020-12-28
Answer
For the Anti-PD-L2 PDCD1LG2 Antibody (A03295), our starting concentration is 2.5 ug/ml for use in IHC-P.
Boster Scientific Support
Answered: 2020-12-29
