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Product Info Summary
| SKU: | A00963 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-IL-22 Antibody
SKU/Catalog Number
A00963
Size
100μl
Form
Liquid
Description
Boster Bio Anti-IL-22 Antibody catalog # A00963. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL-22 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00963)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from the C-terminal region of human IL22. AA range:121-170
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00963 is reactive to IL22 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
20011 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00963 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
Click image to see more details
Western Blot (WB) analysis of Mouse Kidney lysis using IL22 antibody.
Protein Target Info & Infographic
Gene/Protein Information For IL22 (Source: Uniprot.org, NCBI)
Gene Name
IL22
Full Name
Interleukin-22
Weight
20011 MW
Superfamily
IL-10 family
Alternative Names
Interleukin-22;IL-22;Cytokine Zcyto18;IL-10-related T-cell-derived-inducible factor;IL-TIF;IL22;ILTIF, ZCYTO18;UNQ3099/PRO10096; IL22 IL-21, IL-22, IL-D110, IL-TIF, ILTIF, TIFIL-23, TIFa, zcyto18 interleukin 22 interleukin-22|IL-10-related T-cell-derived inducible factor|cytokine Zcyto18
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IL22, check out the IL22 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IL22: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IL-22 Antibody (A00963)
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No publications found for A00963
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Recommended Resources
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-IL-22 Antibody
Question
My question regards to test anti-IL-22 antibody A00963 on rat zone of skin for research purposes, then I may be interested in using anti-IL-22 antibody A00963 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-04-02
Answer
The products we sell, including anti-IL-22 antibody A00963, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-04-02
Question
We are currently using anti-IL-22 antibody A00963 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-17
Answer
The anti-IL-22 antibody (A00963) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-17
Question
We have seen staining in mouse zone of skin. Any tips? Is anti-IL-22 antibody supposed to stain zone of skin positively?
Verified Customer
Verified customer
Asked: 2020-02-05
Answer
From literature zone of skin does express IL22. From Uniprot.org, IL22 is expressed in zone of skin, blood, among other tissues. Regarding which tissues have IL22 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-02-05
Question
My question regards using your anti-IL-22 antibody for interleukin-20 family signaling studies. Has this antibody been tested with western blotting on mouse kidney? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-11-12
Answer
We appreciate your inquiry. This A00963 anti-IL-22 antibody is validated on mouse kidney. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-11-12
Question
Would anti-IL-22 antibody A00963 work for WB with zone of skin?
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
According to the expression profile of zone of skin, IL22 is highly expressed in zone of skin. So, it is likely that anti-IL-22 antibody A00963 will work for WB with zone of skin.
Boster Scientific Support
Answered: 2019-06-26
Question
Do you have a BSA free version of anti-IL-22 antibody A00963 available?
Verified Customer
Verified customer
Asked: 2019-01-21
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-IL-22 antibody A00963 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-01-21
Question
Here is the WB image, lot number and protocol we used for zone of skin using anti-IL-22 antibody A00963. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-11-08
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-08
Question
I was wanting to use your anti-IL-22 antibody for WB for rat zone of skin on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat zone of skin identification?
Verified Customer
Verified customer
Asked: 2018-11-07
Answer
As indicated on the product datasheet, A00963 anti-IL-22 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat zone of skin in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-11-07
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for zone of skin using anti-IL-22 antibody A00963. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-10-01
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-10-01
Question
Would A00963 anti-IL-22 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-08-07
Answer
It shows on the product datasheet, A00963 anti-IL-22 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-08-07
Question
I have a question about product A00963, anti-IL-22 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
R. Li
Verified customer
Asked: 2018-04-23
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00963 anti-IL-22 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-04-23
Question
I see that the anti-IL-22 antibody A00963 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-06-19
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-06-19
Question
Is a blocking peptide available for product anti-IL-22 antibody (A00963)?
R. Krishna
Verified customer
Asked: 2016-09-05
Answer
We do provide the blocking peptide for product anti-IL-22 antibody (A00963). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-09-05
Question
Our lab were happy with the WB result of your anti-IL-22 antibody. However we have seen positive staining in blood secreted. using this antibody. Is that expected? Could you tell me where is IL22 supposed to be expressed?
L. Anderson
Verified customer
Asked: 2016-02-23
Answer
According to literature, blood does express IL22. Generally IL22 expresses in secreted. Regarding which tissues have IL22 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2016-02-23
Question
Is this A00963 anti-IL-22 antibody reactive to the isotypes of IL22?
R. Johnson
Verified customer
Asked: 2013-08-23
Answer
The immunogen of A00963 anti-IL-22 antibody is Synthesized peptide derived from the C-terminal region of human IL-22. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-08-23
