Product Info Summary
SKU: | M03295-1 |
---|---|
Size: | 50 µl |
Reactive Species: | Human |
Host: | Mouse |
Application: | IHC-P, WB |
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Product info
Product Name
Anti-PDCD1LG2 Antibody (N-term)
View all PD-L2/B7-DC/PDCD1LG2 Antibodies
SKU/Catalog Number
M03295-1
Size
50 µl
Description
Boster Bio Anti-PDCD1LG2 Antibody (N-term) (Catalog # M03295-1). Tested in WB, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-PDCD1LG2 Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03295-1)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
2011CT241.19.54
Isotype
IgG1,κ
Immunogen
This PDCD1LG2 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 40-75 amino acids from the N-terminal region of human PDCD1LG2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03295-1 is reactive to PDCD1LG2 in Human
Reconstitution
Calculated molecular weight
30957 Da
Background of PD-L2/B7-DC/PDCD1LG2
Involved in the costimulatory signal, essential for T- cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production (By similarity).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03295-1 is guaranteed for IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:4000
IHC-P: 1:25
Validation Images & Assay Conditions
Click image to see more details
All lanes : Anti-PDCD1LG2 Antibody (N-term) at 1:4000 dilution
Lane 1: Non-transfected 293T whole cell lysate
Lane 2: Transfected PD-L2-transfected 293T whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 31 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Click image to see more details
Immunohistochemical analysis of PDL-2 in untransfected(left) or transfected(right) with 293T cell sections. Cell was fixed with formaldehyde; antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody (1:25) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Protein Target Info & Infographic
Gene/Protein Information For PDCD1LG2 (Source: Uniprot.org, NCBI)
Gene Name
PDCD1LG2
Full Name
Programmed cell death 1 ligand 2
Weight
30957 Da
Superfamily
immunoglobulin superfamily
Alternative Names
Programmed cell death 1 ligand 2, PD-1 ligand 2, PD-L2, PDCD1 ligand 2, Programmed death ligand 2, Butyrophilin B7-DC, B7-DC, CD273, PDCD1LG2, B7DC, CD273, PDCD1L2, PDL2 Pdcd1lg2|B7-DC, Btdc, F730015O22Rik, PD-, PD-L2|programmed cell death 1 ligand 2|programmed cell death 1 ligand 2|PD-1 ligand 2|PDCD1 ligand 2|butyrophilin B7-DC|butyrophilin-like protein|programmed death ligand 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PDCD1LG2, check out the PDCD1LG2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PDCD1LG2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PDCD1LG2 Antibody (N-term) (M03295-1)
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