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Product Info Summary
| SKU: | A00657-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-TIM 3/HAVCR2 Antibody Picoband®
SKU/Catalog Number
A00657-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TIM 3/HAVCR2 Antibody Picoband® catalog # A00657-1. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TIM 3/HAVCR2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00657-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human TIM 3 recombinant protein (Position: S22-G202).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00657-1 is reactive to HAVCR2 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
45-70 kDa
Calculated molecular weight
74585 MW
Background of TIM-3
Hepatitis A virus cellular receptor 2 (HAVCR2), also known as T-cell immunoglobulin and mucin-domain containing-3 (TIM-3), is a protein that in humans is encoded by the HAVCR2 gene. The protein encoded by this gene belongs to the immunoglobulin superfamily, and TIM family of proteins. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas, Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. This protein is a Th1-specific cell surface protein that regulates macrophage activation, and inhibits Th1-mediated auto- and alloimmune responses, and promotes immunological tolerance.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00657-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human Raji whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of TIM 3 using anti-TIM 3 antibody (A00657-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIM 3 antigen affinity purified polyclonal antibody (Catalog # A00657-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TIM 3 at approximately 45-70KD. The expected band size for TIM 3 is at 33KD.
Protein Target Info & Infographic
Gene/Protein Information For HAVCR2 (Source: Uniprot.org, NCBI)
Gene Name
HAVCR2
Full Name
Hepatitis A virus cellular receptor 2
Weight
74585 MW
Superfamily
immunoglobulin superfamily
Alternative Names
Hepatitis A virus cellular receptor 2; HAVcr-2; T-cell immunoglobulin and mucin domain-containing protein 3; TIMD-3; T-cell immunoglobulin mucin receptor 3; TIM-3; T-cell membrane protein 3; HAVCR2; TIM3; TIMD3 HAVCR2 CD366, HAVcr-2, KIM-3, SPTCL, TIM3, TIMD-3, TIMD3, Tim-3 hepatitis A virus cellular receptor 2 hepatitis A virus cellular receptor 2|T cell immunoglobulin mucin 3|T-cell immunoglobulin and mucin domain-containing protein 3|T-cell immunoglobulin mucin family member 3|T-cell immunoglobulin mucin receptor 3|T-cell membrane protein 3|kidney injury molecule-3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HAVCR2, check out the HAVCR2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HAVCR2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TIM 3/HAVCR2 Antibody Picoband® (A00657-1)
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Customer Q&As
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16 Customer Q&As for Anti-TIM 3/HAVCR2 Antibody Picoband®
Question
We are currently using anti-TIM 3/HAVCR2 antibody A00657-1 for human tissue, and we are well pleased with the ELISA results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
The anti-TIM 3/HAVCR2 antibody (A00657-1) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-05-01
Question
I have a question about product A00657-1, anti-TIM 3/HAVCR2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-24
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00657-1 anti-TIM 3/HAVCR2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-24
Question
Do you have a BSA free version of anti-TIM 3/HAVCR2 antibody A00657-1 available?
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TIM 3/HAVCR2 antibody A00657-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-20
Question
Please see the WB image, lot number and protocol we used for embryo umbilical cord blood using anti-TIM 3/HAVCR2 antibody A00657-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-10
Question
We want to test anti-TIM 3/HAVCR2 antibody A00657-1 on human embryo umbilical cord blood for research purposes, then I may be interested in using anti-TIM 3/HAVCR2 antibody A00657-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-16
Answer
The products we sell, including anti-TIM 3/HAVCR2 antibody A00657-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-16
Question
We were well pleased with the WB result of your anti-TIM 3/HAVCR2 antibody. However we have been able to see positive staining in hepatoma membrane using this antibody. Is that expected? Could you tell me where is HAVCR2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-08-30
Answer
According to literature, hepatoma does express HAVCR2. Generally HAVCR2 expresses in membrane. Regarding which tissues have HAVCR2 expression, here are a few articles citing expression in various tissues:
Embryo, and Umbilical cord blood, Pubmed ID: 14702039
Lung, and Pancreas, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-08-30
Question
Is this A00657-1 anti-TIM 3/HAVCR2 antibody reactive to the isotypes of HAVCR2?
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
The immunogen of A00657-1 anti-TIM 3/HAVCR2 antibody is E. coli-derived human TIM 3 recombinant protein (Position: S22-G202). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-29
Question
Will anti-TIM 3/HAVCR2 antibody A00657-1 work for WB with embryo umbilical cord blood?
Verified Customer
Verified customer
Asked: 2018-10-10
Answer
According to the expression profile of embryo umbilical cord blood, HAVCR2 is highly expressed in embryo umbilical cord blood. So, it is likely that anti-TIM 3/HAVCR2 antibody A00657-1 will work for WB with embryo umbilical cord blood.
Boster Scientific Support
Answered: 2018-10-10
Question
Does A00657-1 anti-TIM 3/HAVCR2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-06-12
Answer
As indicated on the product datasheet, A00657-1 anti-TIM 3/HAVCR2 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-06-12
Question
I see that the anti-TIM 3/HAVCR2 antibody A00657-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-05-31
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-05-31
Question
We have observed staining in human lung pancreas. What should we do? Is anti-TIM 3/HAVCR2 antibody supposed to stain lung pancreas positively?
T. Krishna
Verified customer
Asked: 2018-03-13
Answer
From literature lung pancreas does express HAVCR2. From Uniprot.org, HAVCR2 is expressed in layer of synovial tissue, hepatoma, embryo umbilical cord blood, lung pancreas, among other tissues. Regarding which tissues have HAVCR2 expression, here are a few articles citing expression in various tissues:
Embryo, and Umbilical cord blood, Pubmed ID: 14702039
Lung, and Pancreas, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-03-13
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for embryo umbilical cord blood using anti-TIM 3/HAVCR2 antibody A00657-1. Let me know if you need anything else.
G. Zhao
Verified customer
Asked: 2018-01-22
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-01-22
Question
Is a blocking peptide available for product anti-TIM 3/HAVCR2 antibody (A00657-1)?
M. Carter
Verified customer
Asked: 2017-08-28
Answer
We do provide the blocking peptide for product anti-TIM 3/HAVCR2 antibody (A00657-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-08-28
Question
I was wanting to use using your anti-TIM 3/HAVCR2 antibody for negative regulation of t-helper 1 type immune response studies. Has this antibody been tested with western blotting on human raji whole cell lysates? We would like to see some validation images before ordering.
D. Moore
Verified customer
Asked: 2017-08-08
Answer
Thanks for your inquiry. This A00657-1 anti-TIM 3/HAVCR2 antibody is validated on human raji whole cell lysates. It is guaranteed to work for ELISA, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-08-08
Question
I was wanting to use your anti-TIM 3/HAVCR2 antibody for WB for human embryo umbilical cord blood on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human embryo umbilical cord blood identification?
G. Anderson
Verified customer
Asked: 2016-06-02
Answer
You can see on the product datasheet, A00657-1 anti-TIM 3/HAVCR2 antibody has been tested for ELISA, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human embryo umbilical cord blood in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-06-02
Question
We ordered your anti-TIM 3/HAVCR2 antibody for WB on lung pancreas in the past. I am using human, and We want to use the antibody for ELISA next. We want examining lung pancreas as well as hepatoma in our next experiment. Do you have any suggestion on which antibody would work the best for ELISA?
R. Li
Verified customer
Asked: 2014-03-24
Answer
I looked at the website and datasheets of our anti-TIM 3/HAVCR2 antibody and it appears that A00657-1 has been tested on human in both WB and ELISA. Thus A00657-1 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2014-03-24
