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Product Info Summary
| SKU: | M00421-2 |
|---|---|
| Size: | 100μg/vial |
| Reactive Species: | Mouse |
| Host: | Mouse |
| Application: | IHC-P |
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Product info
Product Name
Anti- IL 17A Monoclonal Antibody
View all IL-17/IL-17A Antibodies
SKU/Catalog Number
M00421-2
Size
100μg/vial
Form
Lyophilized
Description
Boster Bio Anti- IL 17A Monoclonal Antibody catalog # M00421-2. Tested in IHC-P applications. This antibody reacts with Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti- IL 17A Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00421-2)
Host
Mouse
Contents
PBS, pH 7.0. Contains no stabilizers or preservatives
Clonality
Monoclonal
Clone Number
AFIF-9
Isotype
Mouse IgG1, κ
Immunogen
Mouse IL-17A cross-linked to OVA
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
M00421-2 is reactive to Il17a in Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Observed Molecular Weight
42 kDa
Calculated molecular weight
46663 MW
Background of IL-17/IL-17A
Interleukin-17A is a protein that in humans is encoded by the IL17A gene. The protein encoded by this gene is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00421-2 is guaranteed for IHC-P Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse
Positive Control
IHC: mouse spleen tissue
Validation Images & Assay Conditions
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Figure 1. IHC analysis of IL17A using anti-IL17A antibody (M00421-2).
IL17A was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/ml mouse anti-IL17A Antibody (M00421-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Il17a (Source: Uniprot.org, NCBI)
Gene Name
Il17a
Full Name
Interleukin-17A
Weight
46663 MW
Superfamily
IL-17 family
Alternative Names
Slit homolog 1 protein; Slit-1; Multiple epidermal growth factor-like domains protein 4; Multiple EGF-like domains protein 4; SLIT1; KIAA0813; MEGF4; SLIL1 IL17A CTLA-8, CTLA8, IL-17, IL-17A, IL17 interleukin 17A interleukin-17A|cytotoxic T-lymphocyte-associated 8|cytotoxic T-lymphocyte-associated protein 8|interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Il17a, check out the Il17a Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Il17a: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti- IL 17A Monoclonal Antibody (M00421-2)
Hello CJ!
M00421-2 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Pirfenidone ameliorates silica-induced lung inflammation and fibrosis in mice by inhibiting the secretion of interleukin-17A
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