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Product Info Summary
| SKU: | PB9152 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-p63/TP63 Antibody Picoband®
SKU/Catalog Number
PB9152
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-p63/TP63 Antibody Picoband® catalog # PB9152. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-p63/TP63 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9152)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human p63 recombinant protein (Position: S311-E680). Human p63 shares 98% amino acid (aa) sequence identity with both mouse and rat p63.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9152 is reactive to TP63 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
75 kDa
Calculated molecular weight
76785 MW
Background of p63/TP73L
Tumor protein p63 (TP63), also known as the p63 or TP73L is a protein that in humans is encoded by the TP63 gene. It is mapped to 3q28. TP63 is a member of the p53 family of transcription factors. This gene encodes for two main isoforms by alternative promoters (TAp63 and ΔNp63). TP63 has been mostly restricted to its apoptotic function and more recently as the guardian of oocyte integrity. It has been found that the combined loss of TP63 and p73 results in the failure of cells containing functional p53 to undergo apoptosis in response to DNA damage. TP63 is an essential regulator of stem cell maintenance in stratified epithelial tissues. Tp63 is also critical for maintaining the progenitor-cell populations that are necessary to sustain epithelial development and morphogenesis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9152 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: human A431 whole cell
IHC: human oesophagus squama cancer tissue
ICC/IF: A431 cell
FCM: A431 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of p63 using anti-p63 antibody (PB9152).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates.
red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-p63 antigen affinity purified polyclonal antibody (Catalog # PB9152) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for p63 at approximately 75 kDa. The expected band size for p63 is at 75 kDa.
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Figure 2. IHC analysis of p63 using anti-p63 antibody (PB9152).
p63 was detected in a paraffin-embedded section of human oesophagus squama cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-p63 Antibody (PB9152) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IF analysis of p63 using anti-p63 antibody (PB9152).
p63 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-p63 Antibody (PB9152) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 4. Flow Cytometry analysis of A431 cells using anti-p63 antibody (PB9152).
Overlay histogram showing A431 cells stained with PB9152 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-p63 Antibody (PB9152, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For TP63 (Source: Uniprot.org, NCBI)
Gene Name
TP63
Full Name
Tumor protein 63
Weight
76785 MW
Superfamily
p53 family
Alternative Names
Tumor protein 63;p63;Chronic ulcerative stomatitis protein;CUSP;Keratinocyte transcription factor KET;Transformation-related protein 63;TP63;Tumor protein p73-like;p73L;p40;p51;TP63;KET, P63, P73H, P73L, TP73L; TP63 AIS, B(p51A), B(p51B), EEC3, KET, LMS, NBP, OFC8, RHS, SHFM4, TP53CP, TP53L, TP73L, p40, p51, p53CP, p63, p73H, p73L tumor protein p63 tumor protein 63|amplified in squamous cell carcinoma|chronic ulcerative stomatitis protein|keratinocyte transcription factor KET|transformation-related protein 63|tumor protein p53-competing protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TP63, check out the TP63 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TP63: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-p63/TP63 Antibody Picoband® (PB9152)
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Customer Q&As
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17 Customer Q&As for Anti-p63/TP63 Antibody Picoband®
Question
We ordered your anti-p63/TP63 antibody for WB on skeletal muscle a few years ago. I am using human, and I plan to use the antibody for IHC next. you antibody examining skeletal muscle as well as keratinocyte skeletal muscle in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2020-05-08
Answer
I have checked the website and datasheets of our anti-p63/TP63 antibody and I see that PB9152 has been tested on human in both WB and IHC. Thus PB9152 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-05-08
Question
My team were well pleased with the WB result of your anti-p63/TP63 antibody. However we have been able to see positive staining in skin of abdomen nucleus using this antibody. Is that expected? Could you tell me where is TP63 supposed to be expressed?
B. Parker
Verified customer
Asked: 2020-04-21
Answer
Based on literature, skin of abdomen does express TP63. Generally TP63 expresses in nucleus. Regarding which tissues have TP63 expression, here are a few articles citing expression in various tissues:
Keratinocyte, and Skeletal muscle, Pubmed ID: 9799841
Lymph, Pubmed ID: 15489334
Placenta, Pubmed ID: 11477076
Prostate, Pubmed ID: 9662346
Skeletal muscle, Pubmed ID: 9662378
Boster Scientific Support
Answered: 2020-04-21
Question
Does PB9152 anti-p63/TP63 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-04-09
Answer
It shows on the product datasheet, PB9152 anti-p63/TP63 antibody as been tested on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-09
Question
I have a question about product PB9152, anti-p63/TP63 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-06
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9152 anti-p63/TP63 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-06
Question
Does anti-p63/TP63 antibody PB9152 work for ICC with skeletal muscle?
M. Lewis
Verified customer
Asked: 2020-03-04
Answer
According to the expression profile of skeletal muscle, TP63 is highly expressed in skeletal muscle. So, it is likely that anti-p63/TP63 antibody PB9152 will work for ICC with skeletal muscle.
Boster Scientific Support
Answered: 2020-03-04
Question
We have been able to see staining in human placenta. Do you have any suggestions? Is anti-p63/TP63 antibody supposed to stain placenta positively?
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
From literature placenta does express TP63. From Uniprot.org, TP63 is expressed in skin of abdomen, keratinocyte skeletal muscle, skeletal muscle, lymph, prostate, placenta, among other tissues. Regarding which tissues have TP63 expression, here are a few articles citing expression in various tissues:
Keratinocyte, and Skeletal muscle, Pubmed ID: 9799841
Lymph, Pubmed ID: 15489334
Placenta, Pubmed ID: 11477076
Prostate, Pubmed ID: 9662346
Skeletal muscle, Pubmed ID: 9662378
Boster Scientific Support
Answered: 2020-02-10
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for skeletal muscle using anti-p63/TP63 antibody PB9152. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-20
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-20
Question
We want to test anti-p63/TP63 antibody PB9152 on human skeletal muscle for research purposes, then I may be interested in using anti-p63/TP63 antibody PB9152 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-20
Answer
The products we sell, including anti-p63/TP63 antibody PB9152, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-20
Question
I have attached the WB image, lot number and protocol we used for skeletal muscle using anti-p63/TP63 antibody PB9152. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-12
Question
We are currently using anti-p63/TP63 antibody PB9152 for human tissue, and we are well pleased with the ICC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on canine tissues as well?
F. Patel
Verified customer
Asked: 2019-01-22
Answer
The anti-p63/TP63 antibody (PB9152) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-01-22
Question
Does PB9152 have more affinity towards TAp3 or towards DeltaNp3 in pathological immunohistochemistry?
Verified Customer
Verified customer
Asked: 2018-02-08
Answer
PB9152 has more affinity towards DeltaNp3 in pathological immunohistochemistry.
Boster Scientific Support
Answered: 2018-02-08
Question
We are interested in using your anti-p63/TP63 antibody for protein homotetramerization studies. Has this antibody been tested with western blotting on squama cancer tissue? We would like to see some validation images before ordering.
H. Jones
Verified customer
Asked: 2017-06-21
Answer
Thank you for your inquiry. This PB9152 anti-p63/TP63 antibody is validated on squama cancer tissue. It is guaranteed to work for IHC, ICC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-06-21
Question
Is this PB9152 anti-p63/TP63 antibody reactive to the isotypes of TP63?
Verified Customer
Verified customer
Asked: 2017-06-19
Answer
The immunogen of PB9152 anti-p63/TP63 antibody is E.coli-derived human p63 recombinant protein (Position: S311-E680). Human p63 shares 98% amino acid (aa) sequence identity with both mouse and rat p63. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-06-19
Question
Is there a BSA free version of anti-p63/TP63 antibody PB9152 available?
P. Banerjee
Verified customer
Asked: 2016-06-08
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-p63/TP63 antibody PB9152 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-06-08
Question
I see that the anti-p63/TP63 antibody PB9152 works with ICC, what is the protocol used to produce the result images on the product page?
S. Singh
Verified customer
Asked: 2014-11-27
Answer
You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2014-11-27
Question
I was wanting to use your anti-p63/TP63 antibody for ICC for human skeletal muscle on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human skeletal muscle identification?
J. Yang
Verified customer
Asked: 2014-08-27
Answer
As indicated on the product datasheet, PB9152 anti-p63/TP63 antibody has been validated for IHC, ICC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human skeletal muscle in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-08-27
Question
Is a blocking peptide available for product anti-p63/TP63 antibody (PB9152)?
L. Collins
Verified customer
Asked: 2013-10-31
Answer
We do provide the blocking peptide for product anti-p63/TP63 antibody (PB9152). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-10-31
