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Product Info Summary
| SKU: | M00167 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Cow, Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-p40 (deltaNp63) (Squamous, Basal & Myoepithelial Cell Marker) TP63 Antibody
SKU/Catalog Number
M00167
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-p40 (deltaNp63) (Squamous, Basal & Myoepithelial Cell Marker) TP63 Antibody (Catalog # M00167). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat, Cow.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-p40 (deltaNp63) (Squamous, Basal & Myoepithelial Cell Marker) TP63 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00167)
Host
Rabbit
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.
Clonality
Polyclonal
Isotype
IgG
Immunogen
A synthetic peptide (ENNAQTQFSEPQY) corresponding to aa5-17 of human p40
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00167 is reactive to TP63 in Cow, Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Calculated molecular weight
76785 MW
Background of p63/TP73L
p40 (p63 delta) is a marker recently determined to be highly specific for squamous basal cells in the immunohistochemistry (IHC) application. The current more routinely recommended marker, p63, appears to have less specificity compared to p40, especially on squamous cell tumors. The ability to differentiate between lung adenocarcinoma vs. squamous cell carcinoma is difficult and has bearing on the different therapeutic avenues for each subtype treatment. p63 antibody's ability to distinguish between the tumor types appears to be inferior when compared to p40. The ability to utilize an antibody probe for p40 as a squamous cell marker bolsters its use for future sub-classification of lung cancers, especially by immunohistochemical techniques.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00167 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Anti-p40 Rabbit Polyclonal Antibody.
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Formalin-fixed, paraffin-embedded Lung Squamous Cell Carcinoma stained with Anti-p40 Rabbit Polyclonal Antibody.
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Western Blot of HEK293 lysate (1) Absence and (2) Presence of immunizing peptide p40 Rabbit PAb.
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SDS-PAGE analysis purified Anti-p40 Rabbit Polyclonal Antibody.
Protein Target Info & Infographic
Gene/Protein Information For TP63 (Source: Uniprot.org, NCBI)
Gene Name
TP63
Full Name
Tumor protein 63
Weight
76785 MW
Superfamily
p53 family
Alternative Names
Tumor protein 63;p63;Chronic ulcerative stomatitis protein;CUSP;Keratinocyte transcription factor KET;Transformation-related protein 63;TP63;Tumor protein p73-like;p73L;p40;p51;TP63;KET, P63, P73H, P73L, TP73L; TP63 AIS, B(p51A), B(p51B), EEC3, KET, LMS, NBP, OFC8, RHS, SHFM4, TP53CP, TP53L, TP73L, p40, p51, p53CP, p63, p73H, p73L tumor protein p63 tumor protein 63|amplified in squamous cell carcinoma|chronic ulcerative stomatitis protein|keratinocyte transcription factor KET|transformation-related protein 63|tumor protein p53-competing protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TP63, check out the TP63 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TP63: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-p40 (deltaNp63) (Squamous, Basal & Myoepithelial Cell Marker) TP63 Antibody (M00167)
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No publications found for M00167
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Customer Q&As
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15 Customer Q&As for Anti-p40 (deltaNp63) (Squamous, Basal & Myoepithelial Cell Marker) TP63 Antibody
Question
Would anti-p40 (deltaNp63) (Squamous, Basal & Myoepithelial Cell Marker) antibody M00167 work on goat IHC with lymph?
Verified Customer
Verified customer
Asked: 2020-03-05
Answer
Our lab technicians have not validated anti-p40 (deltaNp63) (Squamous, Basal & Myoepithelial Cell Marker) antibody M00167 on goat. You can run a BLAST between goat and the immunogen sequence of anti-p40 (deltaNp63) (Squamous, Basal & Myoepithelial Cell Marker) antibody M00167 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat lymph in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-05
Question
We have been able to see staining in mouse lymph. Any tips? Is anti-p40 (deltaNp63) (Squamous, Basal & Myoepithelial Cell Marker) antibody supposed to stain lymph positively?
Verified Customer
Verified customer
Asked: 2020-02-11
Answer
According to literature lymph does express TP63. According to Uniprot.org, TP63 is expressed in skin of abdomen, keratinocyte skeletal muscle, skeletal muscle, lymph, prostate, placenta, among other tissues. Regarding which tissues have TP63 expression, here are a few articles citing expression in various tissues:
Keratinocyte, and Skeletal muscle, Pubmed ID: 9799841
Lymph, Pubmed ID: 15489334
Placenta, Pubmed ID: 11477076
Prostate, Pubmed ID: 9662346
Skeletal muscle, Pubmed ID: 9662378
Boster Scientific Support
Answered: 2020-02-11
Question
Is there a BSA free version of anti-p40 (deltaNp63) (Squamous, Basal & Myoepithelial Cell Marker) antibody M00167 available?
Verified Customer
Verified customer
Asked: 2019-12-26
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-p40 (deltaNp63) (Squamous, Basal & Myoepithelial Cell Marker) antibody M00167 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-26
Question
Here is the WB image, lot number and protocol we used for prostate using anti-p40 (deltaNp63) (Squamous, Basal & Myoepithelial Cell Marker) antibody M00167. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-28
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-28
Question
Is this M00167 anti-p40 (deltaNp63) (Squamous, Basal & Myoepithelial Cell Marker) antibody reactive to the isotypes of TP63?
Verified Customer
Verified customer
Asked: 2019-09-26
Answer
The immunogen of M00167 anti-p40 (deltaNp63) (Squamous, Basal & Myoepithelial Cell Marker) antibody is A synthetic peptide (ENNAQTQFSEPQY) corresponding to aa5-17 of human p40 . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-26
Question
I see that the anti-p40 (deltaNp63) (Squamous, Basal & Myoepithelial Cell Marker) antibody M00167 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-09-09
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-09-09
Question
My question regarding product M00167, anti-p40 (deltaNp63) (Squamous, Basal & Myoepithelial Cell Marker) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-26
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00167 anti-p40 (deltaNp63) (Squamous, Basal & Myoepithelial Cell Marker) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-26
Question
I was wanting to use your anti-p40 (deltaNp63) (Squamous, Basal & Myoepithelial Cell Marker) antibody for IHC for mouse prostate on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse prostate identification?
Verified Customer
Verified customer
Asked: 2019-07-08
Answer
You can see on the product datasheet, M00167 anti-p40 (deltaNp63) (Squamous, Basal & Myoepithelial Cell Marker) antibody has been validated for IHC on bovine, human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse prostate in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-08
Question
My question regards to test anti-p40 (deltaNp63) (Squamous, Basal & Myoepithelial Cell Marker) antibody M00167 on mouse prostate for research purposes, then I may be interested in using anti-p40 (deltaNp63) (Squamous, Basal & Myoepithelial Cell Marker) antibody M00167 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-03-07
Answer
The products we sell, including anti-p40 (deltaNp63) (Squamous, Basal & Myoepithelial Cell Marker) antibody M00167, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-03-07
Question
Would M00167 anti-p40 (deltaNp63) (Squamous, Basal & Myoepithelial Cell Marker) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-11-02
Answer
It shows on the product datasheet, M00167 anti-p40 (deltaNp63) (Squamous, Basal & Myoepithelial Cell Marker) antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-11-02
Question
Is a blocking peptide available for product anti-p40 (deltaNp63) (Squamous, Basal & Myoepithelial Cell Marker) antibody (M00167)?
A. Miller
Verified customer
Asked: 2018-10-02
Answer
We do provide the blocking peptide for product anti-p40 (deltaNp63) (Squamous, Basal & Myoepithelial Cell Marker) antibody (M00167). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-10-02
Question
Would anti-p40 (deltaNp63) (Squamous, Basal & Myoepithelial Cell Marker) antibody M00167 work for IHC with prostate?
Verified Customer
Verified customer
Asked: 2018-08-22
Answer
According to the expression profile of prostate, TP63 is highly expressed in prostate. So, it is likely that anti-p40 (deltaNp63) (Squamous, Basal & Myoepithelial Cell Marker) antibody M00167 will work for IHC with prostate.
Boster Scientific Support
Answered: 2018-08-22
Question
We are currently using anti-p40 (deltaNp63) (Squamous, Basal & Myoepithelial Cell Marker) antibody M00167 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says bovine, human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2018-03-07
Answer
The anti-p40 (deltaNp63) (Squamous, Basal & Myoepithelial Cell Marker) antibody (M00167) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-03-07
Question
Our lab were content with the WB result of your anti-p40 (deltaNp63) (Squamous, Basal & Myoepithelial Cell Marker) antibody. However we have observed positive staining in prostate nucleus using this antibody. Is that expected? Could you tell me where is TP63 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-10-06
Answer
According to literature, prostate does express TP63. Generally TP63 expresses in nucleus. Regarding which tissues have TP63 expression, here are a few articles citing expression in various tissues:
Keratinocyte, and Skeletal muscle, Pubmed ID: 9799841
Lymph, Pubmed ID: 15489334
Placenta, Pubmed ID: 11477076
Prostate, Pubmed ID: 9662346
Skeletal muscle, Pubmed ID: 9662378
Boster Scientific Support
Answered: 2017-10-06
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for prostate using anti-p40 (deltaNp63) (Squamous, Basal & Myoepithelial Cell Marker) antibody M00167. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-07-05
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-07-05
