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Product Info Summary
| SKU: | M14209-1 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Chicken, Cow, Dog, Human, Monkey, Mouse, Rabbit, Rat, Turtle |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-Cytokeratin, Acidic (Type I or LMW) (Epithelial Marker) Monoclonal Antibody
View all Cytokeratin, LMW Antibodies
SKU/Catalog Number
M14209-1
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-Cytokeratin, Acidic (Type I or LMW) (Epithelial Marker) Monoclonal Antibody (Catalog # M14209-1). Tested in Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human, Monkey, Cow, Dog, Rabbit, Mouse, Rat, Chicken, Turtle.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-Cytokeratin, Acidic (Type I or LMW) (Epithelial Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M14209-1)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: SPM115
Isotype
IgG1, kappa
Immunogen
Human epidermal keratin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M14209-1 is reactive to KRT77 in Chicken, Cow, Dog, Human, Monkey, Mouse, Rabbit, Rat, Turtle
Reconstitution
Reconstitute with distilled water.
Observed Molecular Weight
68 kDa
Calculated molecular weight
61901 MW
Background of Cytokeratin, LMW
This monoclonal antibody recognizes the 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50', 48, 46, 45, and 40kDa. MAb SPM116 recognizes the 65-67, 64, 59, 58, 56, and 52kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. SPM115/SPM116 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M14209-1 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Formalin-fixed, paraffin-embedded human Tonsil stained with Anti-Cytokeratin, LMW Monoclonal Antibody (SPM115)
Protein Target Info & Infographic
Gene/Protein Information For KRT77 (Source: Uniprot.org, NCBI)
Gene Name
KRT77
Full Name
Keratin, type II cytoskeletal 1b
Weight
61901 MW
Superfamily
intermediate filament family
Alternative Names
Keratin, type II cytoskeletal 1b;Cytokeratin-1B;CK-1B;Keratin-77;K77;Type-II keratin Kb39;KRT77;KRT1B; KRT77 K1B, KRT1B keratin 77 keratin, type II cytoskeletal 1b|CK-1B|K77|cytokeratin-1B|keratin 1B|keratin 77, type II|type-II keratin Kb39
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KRT77, check out the KRT77 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KRT77: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cytokeratin, Acidic (Type I or LMW) (Epithelial Marker) Monoclonal Antibody (M14209-1)
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