Anti-KRT77 Antibody (Monoclonal, ABM5G22)

Product Name

Anti-KRT77 Antibody (Monoclonal, ABM5G22)

View all Cytokeratin, LMW Antibodies

SKU/Catalog Number

M14209

Size

100 µg

Form

Liquid

Description

Boster Bio Anti-KRT77 Antibody (Monoclonal, ABM5G22) (Catalog# M14209). Tested in WB application(s). This antibody reacts with Human.

Storage & Handling

Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

Cite This Product

Anti-KRT77 Antibody (Monoclonal, ABM5G22) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M14209)

Host

Mouse

Contents

100 μg in 200 μl PBS containing 0.05% BSA and 0.05% sodium azide.

Clonality

Monoclonal

Clone Number

ABM5G22

Isotype

IgG2b Kappa

Immunogen

A partial length recombinant protein of KRT77 (amino acid 133-346)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M14209 is reactive to KRT77 in Human

Applications

M14209 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

61901 MW

Background of Cytokeratin, LMW

This monoclonal antibody recognizes basic (Type II or HMW) cytokeratins, which include 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50', 48, 46, 45, and 40kDa. This monoclonal antibody recognizes the 65-67, 64, 59, 58, 56, and 52kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. SPM115/SPM116 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot: 2-4 µg/ml

Validation Images & Assay Conditions

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Expression analysis of KRT77. Anti-KRT77 antibody (Clone: ABM5G22) was tested at 2 &mu

Gene/Protein Information For KRT77 (Source: Uniprot.org, NCBI)

Alternative Names

Cytokeratin LMW; cytokeratin-1B; keratin 77; keratin, type II cytoskeletal 1b; KRT77; type-II keratin Kb39 KRT77 K1B, KRT1B keratin 77 keratin, type II cytoskeletal 1b|CK-1B|K77|cytokeratin-1B|keratin 1B|keratin 77, type II|type-II keratin Kb39

For more info on KRT77, check out the KRT77 Infographic

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In this infographic, you will see the following information for KRT77: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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