Anti-p63/TP63 Antibody Picoband®

p63/TP73L antibody

Boster Bio Anti-p63/TP63 Antibody catalog # PA2056. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Cited in 3 publication(s).

Product Info Summary

SKU: PA2056
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

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Product Name

Anti-p63/TP63 Antibody Picoband®

View all p63/TP73L Antibodies

SKU/Catalog Number

PA2056

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-p63/TP63 Antibody catalog # PA2056. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-p63/TP63 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2056)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human p63, different from the related rat and mouse sequences by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2056 is reactive to TP63 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

75 kDa

Calculated molecular weight

76785 MW

Background of p63/TP73L

TP63 (Tumor Protein p63), also known as KET, is a protein that in humans is encoded by the TP63 gene. Yang et al. (1998) described the cloning of tumor protein p63, which shows strong homology to the tumor suppressor p53 and the p53-related protein p73. By fluorescence in situ hybridization, Yang et al. (1998) localized the human TP63 gene to chromosome 3q27-q29. Hibi et al. (2000) stated that p53 homologs known variously as p40, p51, p63, and p73L (Trink et al., 1998, Yang et al., 1998, Osada et al., 1998, Senoo et al., 1998) are isoforms of the same gene, which Hibi et al. (2000) referred to as AIS for amplified in squamous cell carcinoma.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA2056 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunocytochemistry , 0.5-1μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human

Positive Control

WB: human A431 whole cell
IHC: human esophageal squamous cell carcinoma tissue, human esophageal squamous cell carcinoma tissue, human lung cancer tissue
ICC/IF: A431 cell
ICC: A549 cell
FCM: A431 cell

Validation Images & Assay Conditions

Gene/Protein Information For TP63 (Source: Uniprot.org, NCBI)

Gene Name

TP63

Full Name

Tumor protein 63

Weight

76785 MW

Superfamily

p53 family

Alternative Names

Tumor protein 63;p63;Chronic ulcerative stomatitis protein;CUSP;Keratinocyte transcription factor KET;Transformation-related protein 63;TP63;Tumor protein p73-like;p73L;p40;p51;TP63;KET, P63, P73H, P73L, TP73L; TP63 AIS, B(p51A), B(p51B), EEC3, KET, LMS, NBP, OFC8, RHS, SHFM4, TP53CP, TP53L, TP73L, p40, p51, p53CP, p63, p73H, p73L tumor protein p63 tumor protein 63|amplified in squamous cell carcinoma|chronic ulcerative stomatitis protein|keratinocyte transcription factor KET|transformation-related protein 63|tumor protein p53-competing protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on TP63, check out the TP63 Infographic

TP63 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TP63: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PA2056 has been cited in 3 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Ozone therapy promotes the differentiation of basal keratinocytes via increasing Tp63‐mediated transcription of KRT10 to improve psoriasis

Zhong H,Ren Z,Wang X,Miao K,Ni W,Meng Y,Lu L,Wang C,Liu W,Deng CX,Xu RH,Chen G.Stagewise keratinocyte differentiation from human embryonic stem cells by defined signal transduction modulators.Int J Biol Sci.2020 Feb 21;16(8):1450-1462.doi:10.7150/ijbs.44414.PMID:32210732;PMCID:PMC7085224.
Species: Human,Mouse
PA2056 usage in article: APP:IHC, SAMPLE:PSCS, DILUTION:NA

Hu L,Sun Y,Luo J,He X,Ye M,Li G,Zhang Y,Bai J,Zhang D,Chang C.Targeting TR4 nuclear receptor with antagonist bexarotene increases docetaxel sensitivity to better suppress the metastatic castration-resistant prostate cancer progression.Oncogene.2020 Feb;39
Species: Human
PA2056 usage in article: APP:IHC, SAMPLE:PCA TISSUE, DILUTION:NA

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6 Customer Q&As for Anti-p63/TP63 Antibody Picoband®

Question

We are interested in using your anti-p63/TP63 antibody for skin morphogenesis studies. Has this antibody been tested with western blotting on a549 cell lysate? We would like to see some validation images before ordering.

D. Thomas

Verified customer

Asked: 2020-04-03

Answer

Thank you for your inquiry. This PA2056 anti-p63/TP63 antibody is validated on hela cell lysate, cell carcinoma tissue, smmc cell lysate, colo320 cell lysate, a549 cell lysate, lung cancer tissue. It is guaranteed to work for IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-04-03

Question

We bought anti-p63/TP63 antibody for IHC on skeletal muscle in a previous experiment. I am using human, and We are going to use the antibody for WB next. My question regards examining skeletal muscle as well as skin of abdomen in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2019-08-22

Answer

I have checked the website and datasheets of our anti-p63/TP63 antibody and it seems that PA2056 has been tested on human in both IHC and WB. Thus PA2056 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-08-22

Question

Our team were content with the WB result of your anti-p63/TP63 antibody. However we have been able to see positive staining in lymph nucleus using this antibody. Is that expected? Could you tell me where is TP63 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-06-06

Answer

According to literature, lymph does express TP63. Generally TP63 expresses in nucleus. Regarding which tissues have TP63 expression, here are a few articles citing expression in various tissues:
Keratinocyte, and Skeletal muscle, Pubmed ID: 9799841
Lymph, Pubmed ID: 15489334
Placenta, Pubmed ID: 11477076
Prostate, Pubmed ID: 9662346
Skeletal muscle, Pubmed ID: 9662378

Boster Scientific Support

Answered: 2019-06-06

Question

We are currently using anti-p63/TP63 antibody PA2056 for rat tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2018-01-04

Answer

The anti-p63/TP63 antibody (PA2056) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-01-04

Question

Does PA2056 have more affinity towards TAp3 or towards DeltaNp3 in pathological immunohistochemistry?

Verified Customer

Verified customer

Asked: 2014-02-06

Answer

PA2056 has more affinity towards DeltaNp3 in pathological immunohistochemistry.

Boster Scientific Support

Answered: 2014-02-06

Question

We have observed staining in mouse skin of abdomen. Are there any suggestions? Is anti-p63/TP63 antibody supposed to stain skin of abdomen positively?

S. Bhatt

Verified customer

Asked: 2013-09-25

Answer

According to literature skin of abdomen does express TP63. According to Uniprot.org, TP63 is expressed in skin of abdomen, keratinocyte skeletal muscle, skeletal muscle, lymph, prostate, placenta, among other tissues. Regarding which tissues have TP63 expression, here are a few articles citing expression in various tissues:
Keratinocyte, and Skeletal muscle, Pubmed ID: 9799841
Lymph, Pubmed ID: 15489334
Placenta, Pubmed ID: 11477076
Prostate, Pubmed ID: 9662346
Skeletal muscle, Pubmed ID: 9662378

Boster Scientific Support

Answered: 2013-09-25

Order DetailsPrice
PA2056

100μg

$370
PA2056-10ug

10μg sample (liquid)

$99
PA2056-Biotin

100 μg Biotin conjugated

$570
PA2056-Cy3

100 μg Cy3 conjugated

$570
PA2056-Dylight488

100 μg Dylight488 conjugated

$570
PA2056-Dylight550

100 μg Dylight550 conjugated

$570
PA2056-Dylight594

100 μg Dylight594 conjugated

$570
PA2056-FITC

100 μg FITC conjugated

$570
PA2056-HRP

100 μg HRP conjugated

$570
PA2056-APC

100 μg APC conjugated

$670
PA2056-PE

100 μg PE conjugated

$670
PA2056-iFluor647

100 μg iFluor647 conjugated

$670
PA2056-carrier-free

Carrier Free

$370

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