Anti-CKAP4 Antibody Picoband®

CKAP4/p63 antibody

Boster Bio Anti-CKAP4 Antibody Picoband® catalog # A02154-1. Tested in WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: A02154-1
Size: 100 μg/vial
Reactive Species: Mouse
Host: Rabbit
Application: WB

Product Name

Anti-CKAP4 Antibody Picoband®

View all CKAP4/p63 Antibodies

SKU/Catalog Number

A02154-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CKAP4 Antibody Picoband® catalog # A02154-1. Tested in WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CKAP4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02154-1)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of mouse CKAP4, different from the related human sequence by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A02154-1 is reactive to Ckap4 in Mouse

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

66 kDa

Calculated molecular weight

63692 MW

Background of CKAP4/p63

Cytoskeleton-associated protein 4, also known as CLIMP63 and p63, is a protein that in humans is encoded by the CKAP4 gene. It is mapped to 12q23.3. CKAP4 is found on the plasma membrane of select cells such as vascular smooth muscle and Type II Greater alveolar lung cells. It has a bimodal distribution. First, CKAP4 is found on the plasma membrane and serves as a receptor for SP-A/surfactant protein-A (in lung) and tPA (in vessels). Second, it is embedded in the membrane of a compartment that links the ER with the Golgi apparatus. This localization is dependent on its ability to form homooligomers, and its presence serves to anchor microtubules and direct the formation of tubular ER.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A02154-1 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Mouse

Positive Control

WB: mouse brain tissue

Validation Images & Assay Conditions

Gene/Protein Information For Ckap4 (Source: Uniprot.org, NCBI)

Gene Name

Ckap4

Full Name

Cytoskeleton-associated protein 4

Weight

63692 MW

Alternative Names

Cytoskeleton-associated protein 4;63-kDa cytoskeleton-linking membrane protein;Climp-63;p63;Ckap4; CKAP4 CLIMP-63, CLIMP63, ERGIC-63, p63 cytoskeleton associated protein 4 cytoskeleton-associated protein 4|63 kDa membrane protein|63-kDa cytoskeleton-linking membrane protein|transmembrane protein (63kD), endoplasmic reticulum/Golgi intermediate compartment|type-II transmembrane protein p63

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Ckap4, check out the Ckap4 Infographic

Ckap4 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Ckap4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

No publications found for A02154-1

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-CKAP4 Antibody Picoband®?

Submit a review and receive an Amazon gift card.

  • $30 for a review with an image

0 Reviews For Anti-CKAP4 Antibody Picoband®

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

7 Customer Q&As for Anti-CKAP4 Antibody Picoband®

Question

We are currently using anti-CKAP4 antibody A02154-1 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says mouse. Is it true that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2019-08-06

Answer

The anti-CKAP4 antibody (A02154-1) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-08-06

Question

I see that the anti-CKAP4 antibody A02154-1 works with WB, what is the protocol used to produce the result images on the product page?

B. Jones

Verified customer

Asked: 2019-07-08

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-07-08

Question

Do you have a BSA free version of anti-CKAP4 antibody A02154-1 available?

Verified Customer

Verified customer

Asked: 2019-03-20

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-CKAP4 antibody A02154-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-03-20

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-CKAP4 antibody A02154-1. Let me know if you need anything else.

G. Mangal

Verified customer

Asked: 2016-08-15

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2016-08-15

Question

Does anti-CKAP4 antibody A02154-1 work for WB with liver?

S. Miller

Verified customer

Asked: 2016-05-02

Answer

According to the expression profile of liver, CKAP4 is highly expressed in liver. So, it is likely that anti-CKAP4 antibody A02154-1 will work for WB with liver.

Boster Scientific Support

Answered: 2016-05-02

Question

We need to test anti-CKAP4 antibody A02154-1 on mouse liver for research purposes, then I may be interested in using anti-CKAP4 antibody A02154-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

B. Li

Verified customer

Asked: 2014-07-23

Answer

The products we sell, including anti-CKAP4 antibody A02154-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2014-07-23

Question

Can you help my question with product A02154-1, anti-CKAP4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

P. Williams

Verified customer

Asked: 2013-08-06

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02154-1 anti-CKAP4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2013-08-06

Order DetailsPrice
A02154-1

100μg

$370
A02154-1-10ug

10μg sample (liquid)

$99
A02154-1-Biotin

100 μg Biotin conjugated

$570
A02154-1-Cy3

100 μg Cy3 conjugated

$570
A02154-1-Dylight488

100 μg Dylight488 conjugated

$570
A02154-1-Dylight550

100 μg Dylight550 conjugated

$570
A02154-1-Dylight594

100 μg Dylight594 conjugated

$570
A02154-1-FITC

100 μg FITC conjugated

$570
A02154-1-HRP

100 μg HRP conjugated

$570
A02154-1-APC

100 μg APC conjugated

$670
A02154-1-PE

100 μg PE conjugated

$670
A02154-1-iFluor647

100 μg iFluor647 conjugated

$670
A02154-1-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
In stock
Order Product
A02154-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.