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Product Info Summary
| SKU: | M00003-1 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-mTOR Rabbit Monoclonal Antibody, Clone#RM274
SKU/Catalog Number
M00003-1
Size
100uL
Form
Liquid
Description
Boster Bio Anti-mTOR Rabbit Monoclonal Antibody, Clone#RM274 (Catalog # M00003-1). Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-mTOR Rabbit Monoclonal Antibody, Clone#RM274 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00003-1)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM274
Isotype
Rabbit IgG
Immunogen
A peptide corresponding to human serine/threonine-protein kinase mTOR.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts to human serine/threonine-protein kinase mTOR. This antibody may also react to mouse or rat mTOR, as predicted by immunogen homology.
Reactive Species
M00003-1 is reactive to MTOR in Human
Reconstitution
Calculated molecular weight
288892 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00003-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:500-1:1000 dilution
WB: 1:1000-1:2000 dilution.
Validation Images & Assay Conditions
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Figure 1. Western Blotting result
Western Blot of HeLa cells lysates using Anti-mTOR Rabbit Monoclonal Antibody (Clone RM274) at a 1:1500 dilution.
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Figure 2. IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-mTOR Rabbit Monoclonal Antibody (Clone RM274) at a 1:1000 dilution.
Protein Target Info & Infographic
Gene/Protein Information For MTOR (Source: Uniprot.org, NCBI)
Gene Name
MTOR
Full Name
Serine/threonine-protein kinase mTOR
Weight
288892 MW
Superfamily
PI3/PI4-kinase family
Alternative Names
Serine/threonine-protein kinase mTOR;2.7.11.1;FK506-binding protein 12-rapamycin complex-associated protein 1;FKBP12-rapamycin complex-associated protein;Mammalian target of rapamycin;mTOR;Mechanistic target of rapamycin;Rapamycin and FKBP12 target 1;Rapamycin target protein 1;MTOR;FRAP, FRAP1, FRAP2, RAFT1, RAPT1; MTOR FRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS mechanistic target of rapamycin kinase serine/threonine-protein kinase mTOR|FK506 binding protein 12-rapamycin associated protein 2|FK506-binding protein 12-rapamycin complex-associated protein 1|FKBP-rapamycin associated protein|FKBP12-rapamycin complex-associated protein 1|mammalian target of rapamycin|mechanistic target of rapamycin (serine/threonine kinase)|rapamycin and FKBP12 target 1|rapamycin associated protein FRAP2|rapamycin target protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MTOR, check out the MTOR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MTOR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-mTOR Rabbit Monoclonal Antibody, Clone#RM274 (M00003-1)
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-mTOR Rabbit Monoclonal Antibody, Clone#RM274
Question
We are currently using anti-mTOR Rabbit Monoclonal antibody, Clone#RM274 M00003-1 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?
E. Wu
Verified customer
Asked: 2020-04-20
Answer
The anti-mTOR Rabbit Monoclonal antibody, Clone#RM274 (M00003-1) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-20
Question
Is a blocking peptide available for product anti-mTOR Rabbit Monoclonal antibody, Clone#RM274 (M00003-1)?
Verified Customer
Verified customer
Asked: 2020-03-18
Answer
We do provide the blocking peptide for product anti-mTOR Rabbit Monoclonal antibody, Clone#RM274 (M00003-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-03-18
Question
Would anti-mTOR Rabbit Monoclonal antibody, Clone#RM274 M00003-1 work for IHC with b-cell?
Verified Customer
Verified customer
Asked: 2020-02-19
Answer
According to the expression profile of b-cell, MTOR is highly expressed in b-cell. So, it is likely that anti-mTOR Rabbit Monoclonal antibody, Clone#RM274 M00003-1 will work for IHC with b-cell.
Boster Scientific Support
Answered: 2020-02-19
Question
My team were content with the WB result of your anti-mTOR Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2019-10-03
Answer
Based on literature, b-cell does express MTOR. Generally MTOR expresses in endoplasmic reticulum membrane. Regarding which tissues have MTOR expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 7809080
Brain, Pubmed ID: 8008069
Cerebellum, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2019-10-03
Question
We ordered your anti-mTOR Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2019-07-02
Answer
I took a look at the website and datasheets of our anti-mTOR Rabbit Monoclonal antibody, Clone
Boster Scientific Support
Answered: 2019-07-02
Question
Is this M00003-1 anti-mTOR Rabbit Monoclonal antibody, Clone#RM274 reactive to the isotypes of MTOR?
D. Walker
Verified customer
Asked: 2019-03-27
Answer
The immunogen of M00003-1 anti-mTOR Rabbit Monoclonal antibody, Clone#RM274 is A peptide corresponding to human serine/threonine-protein kinase mTOR. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-03-27
Question
See below the WB image, lot number and protocol we used for b-cell using anti-mTOR Rabbit Monoclonal antibody, Clone#RM274 M00003-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-03-26
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-03-26
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for b-cell using anti-mTOR Rabbit Monoclonal antibody, Clone#RM274 M00003-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-12-11
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-12-11
Question
We have observed staining in mouse testis. Any tips? Is anti-mTOR Rabbit Monoclonal antibody, Clone#RM274 supposed to stain testis positively?
N. Carter
Verified customer
Asked: 2018-06-06
Answer
From what I have seen in literature testis does express MTOR. From what I have seen in Uniprot.org, MTOR is expressed in testis, brain, cerebellum, b-cell, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have MTOR expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 7809080
Brain, Pubmed ID: 8008069
Cerebellum, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2018-06-06
Question
I was wanting to use your anti-mTOR Rabbit Monoclonal antibody, Clone#RM274 for IHC for human b-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human b-cell identification?
Verified Customer
Verified customer
Asked: 2018-04-02
Answer
It shows on the product datasheet, M00003-1 anti-mTOR Rabbit Monoclonal antibody, Clone#RM274 has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human b-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-04-02
Question
We want using your anti-mTOR Rabbit Monoclonal antibody, Clone#RM274 for germ cell development studies. Has this antibody been tested with western blotting on hela cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2017-10-31
Answer
We appreciate your inquiry. This M00003-1 anti-mTOR Rabbit Monoclonal antibody, Clone#RM274 is tested on hela cells, breast cancer tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-10-31
Question
My question regarding product M00003-1, anti-mTOR Rabbit Monoclonal antibody, Clone#RM274. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
K. Carter
Verified customer
Asked: 2017-10-10
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00003-1 anti-mTOR Rabbit Monoclonal antibody, Clone#RM274, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-10-10
Question
My lab would like to test anti-mTOR Rabbit Monoclonal antibody, Clone#RM274 M00003-1 on human b-cell for research purposes, then I may be interested in using anti-mTOR Rabbit Monoclonal antibody, Clone#RM274 M00003-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
H. Parker
Verified customer
Asked: 2017-09-12
Answer
The products we sell, including anti-mTOR Rabbit Monoclonal antibody, Clone#RM274 M00003-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-09-12
Question
I see that the anti-mTOR Rabbit Monoclonal antibody, Clone#RM274 M00003-1 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-06-08
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-06-08
Question
Is there a BSA free version of anti-mTOR Rabbit Monoclonal antibody, Clone#RM274 M00003-1 available?
B. Lewis
Verified customer
Asked: 2017-01-17
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-mTOR Rabbit Monoclonal antibody, Clone#RM274 M00003-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-01-17
Question
Does M00003-1 anti-mTOR Rabbit Monoclonal antibody, Clone#RM274 work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
C. Walker
Verified customer
Asked: 2015-11-05
Answer
It shows on the product datasheet, M00003-1 anti-mTOR Rabbit Monoclonal antibody, Clone#RM274 as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-11-05
