Anti-Integrin beta 3/ITGB3 Antibody Picoband®

Integrin beta 3/CD61 antibody

Boster Bio Anti-Integrin beta 3/ITGB3 Antibody catalog # PA1627. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Cited in 4 publication(s).

Product Info Summary

SKU: PA1627
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

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Product Name

Anti-Integrin beta 3/ITGB3 Antibody Picoband®

View all Integrin beta 3/CD61 Antibodies

SKU/Catalog Number

PA1627

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Integrin beta 3/ITGB3 Antibody catalog # PA1627. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Integrin beta 3/ITGB3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1627)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Integrin beta 3, identical to the related rat and mouse sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1627 is reactive to ITGB3 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

29 kDa

Calculated molecular weight

87058 MW

Background of Integrin beta 3/CD61

ITGB3 (INTEGRIN, BETA-3), also called GP3A, GPIIIa, CD61, is a protein that in humans is encoded by the ITGB3 gene. GP3A is a cluster of differentiation found on thrombocytes. The ITGB3 complex belongs to the integrin class of cell adhesion molecule receptors that share a common heterodimeric structure with alpha and beta subunits. The GP3A gene is mapped to 17q21.32. And the GP3A gene has 14 exons. The 3-prime exon is larger than 1,700 nucleotides and contains the 3-prime untranslated region. The ITGB3 complex mediates platelet aggregation by acting as a receptor for fibrinogen. Although the ITGB3 is expressed on the cell surface at normal levels and is capable of function following extracellular stimulation, it could not be activated via the "inside-out" signaling pathways.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA1627 is guaranteed for IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat

Positive Control

WB: human U87 whole cell, human HEL whole cell, human Hela whole cell, rat spleen tissue, mouse spleen tissue
IHC: human laryngeal squamous cell carcinoma tissue, human liver cancer tissue, human thyroid cancer tissue, rat lung tissue

Validation Images & Assay Conditions

Gene/Protein Information For ITGB3 (Source: Uniprot.org, NCBI)

Gene Name

ITGB3

Full Name

Integrin beta-3

Weight

87058 MW

Superfamily

integrin beta chain family

Alternative Names

Integrin beta-3;Platelet membrane glycoprotein IIIa;GPIIIa;CD61;ITGB3;GP3A; ITGB3 BDPLT16, BDPLT2, CD61, GP3A, GPIIIa, GT integrin subunit beta 3 integrin beta-3| CD61|integrin beta 3|integrin beta chain, beta 3|integrin, beta 3 (platelet glycoprotein IIIa, CD61)|platelet membrane glycoprotein IIIa

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ITGB3, check out the ITGB3 Infographic

ITGB3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ITGB3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PA1627 has been cited in 4 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Zhou L,Li C,Liu X,Zhang T.Effect of Irisin on LIF and integrin αvβ3 in rats of implantation failure.Reprod Biol Endocrinol.2021 Feb 3;19(1):18.doi:10.1186/s12958-021-00700-9.PMID:33536035;PMCID:PMC7856750.
Species: Rat
PA1627 usage in article: APP:IHC, SAMPLE:UTERINE TISSUE, DILUTION:NA

Correlation of integrin %u03B23 mRNA and vascular endothelial growth factor protein expression profiles with the clinicopathological features and prognosis of %u2026

Study on the Expression and Clinical Significances of Lewis y Antigen and Integrin ?v, ?3 in Epithelial Ovarian Tumors

TGF?3-mediated induction of Periostin facilitates head and neck cancer growth and is associated with metastasis

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3 Customer Q&As for Anti-Integrin beta 3/ITGB3 Antibody Picoband®

Question

Will anti-Integrin beta 3/ITGB3 antibody PA1627 work on bovine WB with left coronary artery?

G. Carter

Verified customer

Asked: 2020-03-09

Answer

Our lab technicians have not tested anti-Integrin beta 3/ITGB3 antibody PA1627 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-Integrin beta 3/ITGB3 antibody PA1627 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine left coronary artery in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-03-09

Question

We are currently using anti-Integrin beta 3/ITGB3 antibody PA1627 for rat tissue, and we are satisfied with the IHC-F results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2019-05-21

Answer

The anti-Integrin beta 3/ITGB3 antibody (PA1627) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-05-21

Question

Does PA1627 anti-Integrin beta 3/ITGB3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-09-06

Answer

As indicated on the product datasheet, PA1627 anti-Integrin beta 3/ITGB3 antibody as been tested on IHC-F. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-09-06

Order DetailsPrice
PA1627

100μg

$370
PA1627-10ug

10μg sample (liquid)

$99
PA1627-Biotin

100 μg Biotin conjugated

$570
PA1627-Cy3

100 μg Cy3 conjugated

$570
PA1627-Dylight488

100 μg Dylight488 conjugated

$570
PA1627-Dylight550

100 μg Dylight550 conjugated

$570
PA1627-Dylight594

100 μg Dylight594 conjugated

$570
PA1627-FITC

100 μg FITC conjugated

$570
PA1627-HRP

100 μg HRP conjugated

$570
PA1627-APC

100 μg APC conjugated

$670
PA1627-PE

100 μg PE conjugated

$670
PA1627-iFluor647

100 μg iFluor647 conjugated

$670
PA1627-carrier-free

Carrier Free

$370

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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