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Product Info Summary
| SKU: | PA2147 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Matrix metalloproteinase-14 MMP14 Antibody Picoband®
View all MMP-14/MT1-MMP Antibodies
SKU/Catalog Number
PA2147
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Matrix metalloproteinase-14 MMP14 Antibody catalog # PA2147. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Matrix metalloproteinase-14 MMP14 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2147)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human MMP14, different from the related mouse and rat sequences by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2147 is reactive to MMP14 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
150 kDa
Calculated molecular weight
65894 MW
Background of MMP-14/MT1-MMP
Matrix metalloproteinase-14, also called MT-MMP 1, is an enzyme that in humans is encoded by the MMP14 gene. The protein encoded by this gene is a member of the membrane-type MMP subfamily. This gene is mapped to 14q11.2. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. This gene may be involved in actin cytoskeleton reorganization by cleaving PTK7. It acts as a positive regulator of cell growth and migration via activation of MMP15.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2147 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: Rat Spleen Tissue, Human Placenta Tissue, HELA Whole Cell, A549 Whole Cell
IHC: Human Intestinal Cancer Tissue, Human Placenta Tissue, Rat Cardiac Muscle Tissue
Validation Images & Assay Conditions
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Anti-MMP14 antibody, PA2147, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
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Anti-MMP14 antibody, PA2147, IHC(P)
IHC(P): Human Placenta Tissue
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Anti-MMP14 antibody, PA2147, IHC(P)
IHC(P): Rat Cardiac Muscle Tissue
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Anti-MMP14 antibody, PA2147, Western blotting
All lanes: Anti MMP14 (PA2147) at 0.5ug/ml
Lane 1: Rat Spleen Tissue Lysate at 50ug
Lane 2: Human Placenta Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: A549 Whole Cell Lysate at 40ug
Predicted bind size: 66KD
Observed bind size: 150KD
Protein Target Info & Infographic
Gene/Protein Information For MMP14 (Source: Uniprot.org, NCBI)
Gene Name
MMP14
Full Name
Matrix metalloproteinase-14
Weight
65894 MW
Superfamily
peptidase M10A family
Alternative Names
Matrix metalloproteinase-14;MMP-14;3.4.24.80;MMP-X1;Membrane-type matrix metalloproteinase 1;MT-MMP 1;MTMMP1;Membrane-type-1 matrix metalloproteinase;MT1-MMP;MT1MMP;MMP14; MMP14 MMP-14, MMP-X1, MT-MMP, MT-MMP 1, MT1-MMP, MT1MMP, MTMMP1, WNCHRS matrix metallopeptidase 14 matrix metalloproteinase-14|matrix metallopeptidase 14 (membrane-inserted)|membrane type 1 metalloprotease|membrane-type-1 matrix metalloproteinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MMP14, check out the MMP14 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MMP14: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Matrix metalloproteinase-14 MMP14 Antibody Picoband® (PA2147)
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Customer Q&As
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16 Customer Q&As for Anti-Matrix metalloproteinase-14 MMP14 Antibody Picoband®
Question
We have seen staining in human placenta. What should we do? Is anti-MMP14 antibody supposed to stain placenta positively?
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
According to literature placenta does express MMP14. According to Uniprot.org, MMP14 is expressed in endocervix, placenta, lung, tongue, skin, melanoma, among other tissues. Regarding which tissues have MMP14 expression, here are a few articles citing expression in various tissues:
Lung, Pubmed ID: 7649159
Melanoma, Pubmed ID: 17081065
Placenta, Pubmed ID: 7721107, 8015608
Skin, Pubmed ID: 15489334
Tongue, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-02-13
Question
I have attached the WB image, lot number and protocol we used for endocervix using anti-MMP14 antibody PA2147. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-01-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-22
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for endocervix using anti-MMP14 antibody PA2147. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-26
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-26
Question
Our lab were well pleased with the WB result of your anti-MMP14 antibody. However we have seen positive staining in endocervix membrane using this antibody. Is that expected? Could you tell me where is MMP14 supposed to be expressed?
B. Thomas
Verified customer
Asked: 2019-12-23
Answer
Based on literature, endocervix does express MMP14. Generally MMP14 expresses in membrane. Regarding which tissues have MMP14 expression, here are a few articles citing expression in various tissues:
Lung, Pubmed ID: 7649159
Melanoma, Pubmed ID: 17081065
Placenta, Pubmed ID: 7721107, 8015608
Skin, Pubmed ID: 15489334
Tongue, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-12-23
Question
Do you have a BSA free version of anti-MMP14 antibody PA2147 available?
Verified Customer
Verified customer
Asked: 2019-10-31
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-MMP14 antibody PA2147 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-31
Question
Our lab want to know about to test anti-MMP14 antibody PA2147 on human endocervix for research purposes, then I may be interested in using anti-MMP14 antibody PA2147 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
The products we sell, including anti-MMP14 antibody PA2147, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-25
Question
Our lab want to know about using your anti-MMP14 antibody for embryonic cranial skeleton morphogenesis studies. Has this antibody been tested with western blotting on hela whole cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-07-16
Answer
Thank you for your inquiry. This PA2147 anti-MMP14 antibody is validated on human placenta tissue, intestinal cancer tissue, tissue lysate, rat spleen tissue, cardiac muscle tissue, hela whole cell lysate, a549 whole cell lysate. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-07-16
Question
Will anti-MMP14 antibody PA2147 work for WB with endocervix?
Verified Customer
Verified customer
Asked: 2018-12-14
Answer
According to the expression profile of endocervix, MMP14 is highly expressed in endocervix. So, it is likely that anti-MMP14 antibody PA2147 will work for WB with endocervix.
Boster Scientific Support
Answered: 2018-12-14
Question
We are currently using anti-MMP14 antibody PA2147 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2018-11-20
Answer
The anti-MMP14 antibody (PA2147) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-11-20
Question
Does PA2147 anti-MMP14 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-10-24
Answer
As indicated on the product datasheet, PA2147 anti-MMP14 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-10-24
Question
We have tried in the past anti-MMP14 antibody for IHC on endocervix last year. I am using rat, and We are going to use the antibody for WB next. I was wanting to use examining endocervix as well as melanoma in our next experiment. Could give a recommendation on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2018-04-09
Answer
I have checked the website and datasheets of our anti-MMP14 antibody and I see that PA2147 has been validated on rat in both IHC and WB. Thus PA2147 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-04-09
Question
I see that the anti-MMP14 antibody PA2147 works with WB, what is the protocol used to produce the result images on the product page?
G. Lewis
Verified customer
Asked: 2018-03-01
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-03-01
Question
My question regarding product PA2147, anti-MMP14 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
O. Brown
Verified customer
Asked: 2018-01-31
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2147 anti-MMP14 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-01-31
Question
Is a blocking peptide available for product anti-MMP14 antibody (PA2147)?
Verified Customer
Verified customer
Asked: 2017-11-03
Answer
We do provide the blocking peptide for product anti-MMP14 antibody (PA2147). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-11-03
Question
Is this PA2147 anti-MMP14 antibody reactive to the isotypes of MMP14?
S. Thomas
Verified customer
Asked: 2015-04-16
Answer
The immunogen of PA2147 anti-MMP14 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human MMP14(567-582aa TPRRLLYCQRSLLDKV), different from the related mouse and rat sequences by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-04-16
Question
I was wanting to use your anti-MMP14 antibody for WB for human endocervix on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human endocervix identification?
M. Roberts
Verified customer
Asked: 2014-06-30
Answer
As indicated on the product datasheet, PA2147 anti-MMP14 antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human endocervix in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-06-30
