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Product Info Summary
| SKU: | EK0463 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin) , saliva and urine. |
Product info
Product Name
Human MMP-7/Matrilysin ELISA Kit PicoKine®
SKU/Catalog Number
EK0463
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human MMP-7 ELISA Kit PicoKine® (96 Tests). Quantitate Human MMP7 in cell culture supernatants, serum, plasma (heparin) , saliva and urine. Sensitivity: 6pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human MMP-7/Matrilysin ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0463)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: L18-K267
Sensitivity
<6 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0463 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0463 is reactive to MMP7 in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin) , saliva and urine.
Application Guarantee
EK0463 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of MMP-7
Matrix metalloproteinase-7 (MMP-7) previously called putative metalloproteinase I (PUMP1) or matrilysin. The PUMP1 gene has been identified through studies of collagenase-related connective-tissue-degrading metalloproteinases produced by human tumors. The PUMP I protein has 267 amino acids and is significantly shorter than stromelysin or collagenase (477 and 469 amino acids, respectively). Matrix metalloproteinases play a crucial role in tumor invasion and metastasis. Matrilysin, a member of the matrix metalloproteinase family, is structurally different from the other matrix metalloproteinases by virtue of the absence of a conserved COOH-terminal protein domain. In addition, matrilysin mRNA is regulated in a specific and distinct manner in normal and malignant tissues. Matrilysin has been shown to correlate with nodal or distant metastasis in colorectal carcinomas; however, its implication in early invasive colorectal carcinomas has not been determined.1 Matrilysin is also a mediator of pulmonary fibrosis and a potential therapeutic target. The standard product used in this kit is recombinant human MMP-7, consisting of 250 amino acids with the molecular mass of 28KDa. The detected MMP-7 includes zymogen and active enzyme.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0463-CAP | Anti-Human MMP7 Pre-coated 96-well strip microplate | 1 |
| EK0463-ST | Human MMP7 Standard | 2 vials, 10 ng/tube |
| EK0463-DA | Human MMP7 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.021 | 0.103 | 0.161 | 0.281 | 0.577 | 0.972 | 1.488 | 2.049 |
Data Example Images
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Human MMP-7 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect MMP-7, Dilution ratio of 1:1, concentration in serum and plasma is 0.85-5.28 ng/ml..
Some articles we found to cite concentrations of MMP-7 in samples: 24336111 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human MMP-7/Matrilysin ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 272 | 1042 | 5090 | 256 | 1008 | 5032 |
| Standard deviation | 12.51 | 43.76 | 336.64 | 15.36 | 47.37 | 412.62 |
| CV (%) | 4.6% | 4.2% | 6.6% | 6% | 4.7% | 8.2% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of MMP-7 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 272 | 280 | 250 | 279 | 270 | 12.09 | 4.4% |
| Sample 2 | 1042 | 1051 | 1050 | 1108 | 1062 | 26.35 | 2.4% |
| Sample 3 | 5090 | 5484 | 5287 | 4635 | 5124 | 314.81 | 6.1% |
Protein Target Info & Infographic
Gene/Protein Information For MMP7 (Source: Uniprot.Org, NCBI)
Gene Name
MMP7
Full Name
Matrilysin
Weight
29677 MW
Superfamily
peptidase M10A family
Alternative Names
Matrilysin;3.4.24.23;Matrin;Matrix metalloproteinase-7;MMP-7;Pump-1 protease;Uterine metalloproteinase;MMP7;MPSL1, PUMP1; MMP7 MMP-7, MPSL1, PUMP-1 matrix metallopeptidase 7 matrilysin|matrin|matrix metallopeptidase 7 (matrilysin, uterine)|matrix metalloproteinase 7 (matrilysin, uterine)|matrix metalloproteinase-7|pump-1 protease|uterine matrilysin|uterine metalloproteinase
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on MMP7, check out the MMP7 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MMP7: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human MMP-7/Matrilysin ELISA Kit PicoKine® (EK0463)
Hello CJ!
EK0463 has been cited in 6 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Increased levels of thymosin β4 in synovial fluid of patients with rheumatoid arthritis: association of thymosin β4 with other factors that are involved in inflammation and bone erosion in joints
CCR10 activation stimulates the invasion and migration of breast cancer cells through the ERK1/2/MMP-7 signaling pathway
Implication of MMP-9 and urokinase plasminogen activator (uPA) in the activation of pro-matrix metalloproteinase (MMP)-13
Zhu, G., Lin, C., Cheng, Z., Wang, Q., Hoffman, R. M., Singh, S. R., ... & Ye, J. (2020). TRAF6-Mediated Inflammatory Cytokines Secretion in LPS-induced Colorectal Cancer Cells Is Regulated by miR-140. Cancer Genomics-Proteomics, 17(1), 23-33.
Sun Y, Chen Y, Li S, Lei Y, Xu D, Jiang N, Zhang Y, Cao J, Ke Z. Int J Nanomedicine. 2017 Aug 31;12:6399-6412. doi: 10.2147/IJN.S144033. eCollection 2017. NanoVelcro-captured CTC number concomitant with enhanced serum levels of MMP7 and MMP9 enabl...
Kim Ks, Lee Ya, Choi Hm, Yoo Mc, Yang Hi. Rheumatol Int. 2012 Oct;32(10):3069-75. Epub 2011 Sep 13. Implication Of Mmp-9 And Urokinase Plasminogen Activator (Upa) In The Activation Of Pro-Matrix Metalloproteinase (Mmp)-13.
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
8 Customer Q&As for Human MMP-7/Matrilysin ELISA Kit PicoKine®
Question
Q: Can MMP-7 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
T. Garcia
Verified customer
Asked: 2020-07-24
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-07-24
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2020-06-14
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-06-14
Question
Q: how to analyze ELISA data? I have performed MMP-7 level in saliva.
C. Kaur
Verified customer
Asked: 2020-05-04
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-05-04
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification MMP-7 using the EK0463 Human MMP-7 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-01-20
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2020-01-20
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2018-11-27
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-11-27
Question
Q: if the enzyme conjugated MMP-7 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the MMP-7 antigen?
W. Varma
Verified customer
Asked: 2018-07-05
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-07-05
Question
Q: which procedure should I follow in order to thaw whole blood sample for MMP-7 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2018-06-07
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test MMP-7 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2018-06-07
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
H. Mehta
Verified customer
Asked: 2016-05-25
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2016-05-25
