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Product Info Summary
| SKU: | EK1692 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
Product info
Product Name
Human Pro-collagen Iα1/COL1A1 ELISA Kit PicoKine®
SKU/Catalog Number
EK1692
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Pro-collagen Iα1/COL1A1 ELISA Kit PicoKine® (96 Tests). Quantitate Human COL1A1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml . The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Pro-collagen Iα1/COL1A1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1692)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: CHO; Immunogen sequence: Q23-K277 + G1094-L1464
Sensitivity
<10 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1692 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1692 is reactive to COL1A1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
EK1692 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of COL1A1
Collagen, type I, alpha 1, also known as COL1A1, is a human gene that encodes the major component of type I collagen, the fibrillar collagen found in most connective tissues, including cartilage. This gene is mapped to 17q21.33. And this gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1692-CAP | Anti-Human COL1A1 Pre-coated 96-well strip microplate | 1 |
| EK1692-ST | Human COL1A1 Standard | 2 vials, 10 ng/tube |
| EK1692-DA | Human COL1A1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.026 | 0.09 | 0.119 | 0.215 | 0.374 | 0.716 | 1.249 | 2.052 |
Data Example Images
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Human COL1A1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect COL1A1, Dilution ratio of 1:2000, concentration in serum and plasma is 594-1000 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Pro-collagen Iα1/COL1A1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 105 | 360 | 1669 | 78 | 251 | 1684 |
| Standard deviation | 3.03 | 19.01 | 123.34 | 5.65 | 11.55 | 105.59 |
| CV(%) | 2.9% | 5.4% | 7.4% | 7.2% | 4.6% | 6.3% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of COL1A1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 95 | 94 | 96 | 98 | 95 | 1.47 | 1.5% |
| Sample 2 | 317 | 320 | 299 | 298 | 308 | 10.06 | 3.2% |
| Sample 3 | 1043 | 1012 | 1051 | 1042 | 1037 | 14.84 | 1.4% |
Protein Target Info & Infographic
Gene/Protein Information For COL1A1 (Source: Uniprot.Org, NCBI)
Gene Name
COL1A1
Full Name
Collagen alpha-1(I) chain
Weight
138.941kDa
Superfamily
fibrillar collagen family
Alternative Names
Collagen alpha-1 (I) chain; Alpha-1 type I collagen; COL1A1 COL1A1 CAFYD, EDSARTH1, EDSC, OI1, OI2, OI3, OI4 collagen type I alpha 1 chain collagen alpha-1(I) chain|alpha-1 type I collagen|alpha1(I) procollagen|collagen alpha 1 chain type I|collagen alpha-1(I) chain preproprotein|collagen of skin, tendon and bone, alpha-1 chain|collagen, type I, alpha 1|pro-alpha-1 collagen type 1|type I proalpha 1|type I procollagen alpha 1 chain
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on COL1A1, check out the COL1A1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for COL1A1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Pro-collagen Iα1/COL1A1 ELISA Kit PicoKine® (EK1692)
Hello CJ!
EK1692 has been cited in 8 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Protective Effects of Ginseng Proteins on Photoaging of Mouse Fibroblasts Induced by UVA
Surface Epitaxial Crystallization-Directed Nanotopography for Accelerating Preosteoblast Proliferation and Osteogenic Differentiation
The excitotoxity of NMDA receptor NR2D subtype mediates human fetal lung fibroblasts proliferation and collagen production
Uncarboxylated osteocalcin promotes osteogenic differentiation of mouse bone marrow–derived mesenchymal stem cells by activating the Erk‐Smad/β‐catenin signalling pathways
Yu WY,Cai W,Ying HZ, Zhang WY,Zhang HH,Yu CH.Exogenous Plant gma-miR-159a, Identified by miRNA Library Functional Screening, Ameliorated Hepatic Stellate Cell Activation and Inflammation via Inhibiting GSK-3β-Mediated Pathways.J Inflamm Res.2021;14:2157-2172https://doi.org/10.2147/JIR.S304828
Species: Human,Mouse
EK1692 usage in article: APP:ELISA, SAMPLE:HSCS, DILUTION:NA
MiR-200a ameliorates peritoneal fibrosis and functional deterioration in a rat model of peritoneal dialysis Xin Wei, et al. Int Urol Nephrol. 2019; 51(5): 889–896. Published online 2019 Mar 19. doi: 10.1007/s11255-019-02122-4
Murine and Chinese cobra venom%u2011derived nerve growth factor stimulate chondrogenic differentiation of BMSCs in vitro: A comparative study
Lv C, Wu C, Zhou Yh, Shao Y, Wang G, Wang Qy. Int J Endocrinol. 2014;2014:658589. Doi: 10.1155/2014/658589. Epub 2014 Oct 30. Alpha Lipoic Acid Modulated High Glucose-Induced Rat Mesangial Cell Dysfunction Via Mtor/P70S6K/4E-Bp1 Pathway.
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
8 Customer Q&As for Human Pro-collagen Iα1/COL1A1 ELISA Kit PicoKine®
Question
Q: how to proceed with the analysis of ELISA data? I measured COL1A1 level in plasma.
B. Walker
Verified customer
Asked: 2020-12-09
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-12-09
Question
Q: how to thaw whole blood sample for COL1A1 ELISA after freezing?
W. Davis
Verified customer
Asked: 2020-10-29
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test COL1A1 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2020-10-29
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2020-09-02
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-09-02
Question
Is there a specific cell number recommended per sample/volume for EK1692?
Verified customer
Asked: 2019-10-28
Answer
The cell concentration for the Human Pro-Collagen I Alpha 1/COL1A1 ELISA Kit PicoKine® (EK1692) is recommended to be 10 raised to the 6 cells/mL.
Boster Scientific Support
Answered: 2019-10-29
Question
Q: Can COL1A1 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
T. Zhang
Verified customer
Asked: 2019-06-23
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2019-06-23
Question
Could you please let us know current stock of below item? 1) cat # EK1692, 1EA
Verified Customer
Verified customer
Asked: 2018-12-26
Answer
We do not have any EK1692 in stock. It takes 3 business days to manufacture a new lot after we receive the order.
Boster Scientific Support
Answered: 2018-12-26
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
M. Harris
Verified customer
Asked: 2016-10-31
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2016-10-31
Question
Q: What is the optimal O.D. value for COL1A1 ELISA kit? I used your COL1A1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain COL1A1 even though the O.D. values are not very high?
H. Varma
Verified customer
Asked: 2016-02-19
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this COL1A1 ELISA kit to have sensitivity of 10pg/ml , that means the minimum amount of COL1A1 that can be declared/interpreted as positive by the above standard is 10pg/ml .
Boster Scientific Support
Answered: 2016-02-19
