Click image to see more details
Product Info Summary
| SKU: | A04624-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IHC |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-LAMA5/Laminin Alpha 5 Antibody
View all Laminin alpha 5 Antibodies
SKU/Catalog Number
A04624-1
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-LAMA5/Laminin Alpha 5 Antibody (Catalog # A04624-1). Tested in IHC applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-LAMA5/Laminin Alpha 5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04624-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human LAMA5.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04624-1 is reactive to LAMA5 in Human, Mouse
Reconstitution
Calculated molecular weight
399737 MW
Background of Laminin alpha 5
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Deloukas P., ure 414:865-871(2001).
Nagase T., DNA Res. 8:179-187(2001).
Nagase T., DNA Res. 5:31-39(1998)
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04624-1 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using LAMA5 antibody A04624-1.
Protein Target Info & Infographic
Gene/Protein Information For LAMA5 (Source: Uniprot.org, NCBI)
Gene Name
LAMA5
Full Name
Laminin subunit alpha-5
Weight
399737 MW
Alternative Names
Laminin subunit alpha-5;Laminin-10 subunit alpha;Laminin-11 subunit alpha;Laminin-15 subunit alpha;LAMA5;KIAA0533, KIAA1907; LAMA5 laminin subunit alpha 5 laminin subunit alpha-5|laminin alpha-5 chain|laminin-10 subunit alpha|laminin-11 subunit alpha|laminin-15 subunit alpha
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LAMA5, check out the LAMA5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LAMA5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LAMA5/Laminin Alpha 5 Antibody (A04624-1)
Hello CJ!
No publications found for A04624-1
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-LAMA5/Laminin Alpha 5 Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-LAMA5/Laminin Alpha 5 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
13 Customer Q&As for Anti-LAMA5/Laminin Alpha 5 Antibody
Question
I see that the anti-LAMA5/Laminin Alpha 5 antibody A04624-1 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-04-24
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-04-24
Question
Would anti-LAMA5/Laminin Alpha 5 antibody A04624-1 work on primate IHC with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2020-03-23
Answer
Our lab technicians have not tested anti-LAMA5/Laminin Alpha 5 antibody A04624-1 on primate. You can run a BLAST between primate and the immunogen sequence of anti-LAMA5/Laminin Alpha 5 antibody A04624-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate leukemic t-cell in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-23
Question
Will A04624-1 anti-LAMA5/Laminin Alpha 5 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-12
Answer
It shows on the product datasheet, A04624-1 anti-LAMA5/Laminin Alpha 5 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-12
Question
I have attached the WB image, lot number and protocol we used for leukemic t-cell using anti-LAMA5/Laminin Alpha 5 antibody A04624-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-26
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-26
Question
Is a blocking peptide available for product anti-LAMA5/Laminin Alpha 5 antibody (A04624-1)?
Verified Customer
Verified customer
Asked: 2020-02-03
Answer
We do provide the blocking peptide for product anti-LAMA5/Laminin Alpha 5 antibody (A04624-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-02-03
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-LAMA5/Laminin Alpha 5 antibody A04624-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-04
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-04
Question
My question regarding product A04624-1, anti-LAMA5/Laminin Alpha 5 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-18
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04624-1 anti-LAMA5/Laminin Alpha 5 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-18
Question
We are currently using anti-LAMA5/Laminin Alpha 5 antibody A04624-1 for mouse tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-09
Answer
The anti-LAMA5/Laminin Alpha 5 antibody (A04624-1) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-09
Question
Do you have a BSA free version of anti-LAMA5/Laminin Alpha 5 antibody A04624-1 available?
Verified Customer
Verified customer
Asked: 2018-05-16
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-LAMA5/Laminin Alpha 5 antibody A04624-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-05-16
Question
I was wanting to use your anti-LAMA5/Laminin Alpha 5 antibody for IHC for human leukemic t-cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human leukemic t-cell identification?
Verified Customer
Verified customer
Asked: 2017-12-20
Answer
You can see on the product datasheet, A04624-1 anti-LAMA5/Laminin Alpha 5 antibody has been tested for IHC on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-12-20
Question
Will anti-LAMA5/Laminin Alpha 5 antibody A04624-1 work for IHC with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2017-10-13
Answer
According to the expression profile of leukemic t-cell, LAMA5 is highly expressed in leukemic t-cell. So, it is likely that anti-LAMA5/Laminin Alpha 5 antibody A04624-1 will work for IHC with leukemic t-cell.
Boster Scientific Support
Answered: 2017-10-13
Question
Our lab want to know about to test anti-LAMA5/Laminin Alpha 5 antibody A04624-1 on human leukemic t-cell for research purposes, then I may be interested in using anti-LAMA5/Laminin Alpha 5 antibody A04624-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Banerjee
Verified customer
Asked: 2016-10-17
Answer
The products we sell, including anti-LAMA5/Laminin Alpha 5 antibody A04624-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-10-17
Question
Is this A04624-1 anti-LAMA5/Laminin Alpha 5 antibody reactive to the isotypes of LAMA5?
J. Collins
Verified customer
Asked: 2016-09-27
Answer
The immunogen of A04624-1 anti-LAMA5/Laminin Alpha 5 antibody is Synthesized peptide derived from internal of human LAMA5. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-09-27
