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Product Info Summary
| SKU: | A00656 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-MMP14 Antibody (N-term)
View all MMP-14/MT1-MMP Antibodies
SKU/Catalog Number
A00656
Size
80 µl
Form
Liquid
Description
Boster Bio Anti-MMP14 Antibody (N-term) (Catalog # A00656). Tested in WB, Flow Cytometry, IHC-P application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-MMP14 Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00656)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This MMP14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 145-174 amino acids from the N-terminal region of human MMP14.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00656 is reactive to MMP14 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
65894 Da
Background of MMP-14/MT1-MMP
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP14 seems to specifically activate progelatinase A, and may thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. Expression is significant in stromal cells of colon, breast, and head and neck.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00656 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC-P: 1:25
FC: 1:10-1:50
Validation Images & Assay Conditions
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All lanes : Anti-MMP14 Antibody (N-term) at 1:2000 dilution
Lane 1: A2058 whole cell lysate
Lane 2: HT-1080 whole cell lysate
Lane 3: MCF-7 whole cell lysate
Lane 4: NCI-H292 whole cell lysate
Lane 5: Mouse kidney lysate
Lane 5: Rat kidney lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 66 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Anti-MMP14 Antibody (N-term) at 1:2000 dilution + Mouse lung lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 66 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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A00656 staining MMP14 in human lung adenocarcinoma tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
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Flow cytometric analysis of MCF-7 cells using MMP14 Antibody (N-term) (bottom histogram) compared to a negative control (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Protein Target Info & Infographic
Gene/Protein Information For MMP14 (Source: Uniprot.org, NCBI)
Gene Name
MMP14
Full Name
Matrix metalloproteinase-14
Weight
65894 Da
Superfamily
peptidase M10A family
Alternative Names
Matrix metalloproteinase-14, MMP-14, MMP-X1, Membrane-type matrix metalloproteinase 1, MT-MMP 1, MTMMP1, Membrane-type-1 matrix metalloproteinase, MT1-MMP, MT1MMP, MMP14 MMP14 MMP-14, MMP-X1, MT-MMP, MT-MMP 1, MT1-MMP, MT1MMP, MTMMP1, WNCHRS matrix metallopeptidase 14 matrix metalloproteinase-14|matrix metallopeptidase 14 (membrane-inserted)|membrane type 1 metalloprotease|membrane-type-1 matrix metalloproteinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MMP14, check out the MMP14 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MMP14: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MMP14 Antibody (N-term) (A00656)
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