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Product Info Summary
| SKU: | M00785 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IP, WB |
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Product info
Product Name
Anti-MAD2L1/Mad2 Monoclonal Antibody
SKU/Catalog Number
M00785
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-MAD2L1/Mad2 Monoclonal Antibody (Catalog # M00785). Tested in IP, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-MAD2L1/Mad2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00785)
Host
Mouse
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Monoclonal
Clone Number
Clone: 17D10 IgG1
Isotype
IgG1
Immunogen
This protein A purified monoclonal antibody was produced by repeated immunizations with full-length recombinant human MAD2L1 protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00785 is reactive to MAD2L1 in Human
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
23510 MW
Background of MAD2L1
MAD2L1 (also called mitotic spindle assembly checkpoint protein, MAD2A, MAD2-like 1 and HsMAD2) is a component of the mitotic spindle assembly checkpoint monitors the process of kinetochore-spindle attachment and delays the onset of anaphase when this process is not complete. MAD2L1 inhibits the activity of the anaphase-promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. This protein has a nuclear localization. A MAD2 pseudogene has been mapped to chromosome 14.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00785 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:5,000 - 1:20,000
IP: 1:100
WB: 1:200 - 1:2,000
Validation Images & Assay Conditions
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Western blot analysis of MAD2L1 expression in HeLa whole cell lysate. MAD2L1 at 24KD was detected using mouse anti-MAD2L1 monoclonal antibody (Catalog # M00785) at 1:200. IRDye00 fluorescence image was captured using the Odyssey
Protein Target Info & Infographic
Gene/Protein Information For MAD2L1 (Source: Uniprot.org, NCBI)
Gene Name
MAD2L1
Full Name
Mitotic spindle assembly checkpoint protein MAD2A
Weight
23510 MW
Superfamily
MAD2 family
Alternative Names
MAD2L1, Mitotic arrest deficient yeast homolog like 1, Mitotic spindle assembly checkpoint protein MAD2L1 HSMAD2, MAD2 mitotic arrest deficient 2 like 1 mitotic spindle assembly checkpoint protein MAD2A|MAD2 (mitotic arrest deficient, yeast, homolog)-like 1|MAD2 mitotic arrest deficient-like 1|MAD2-like protein 1|mitotic arrest deficient 2-like protein 1|mitotic arrest deficient, yeast, homolog-like 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAD2L1, check out the MAD2L1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAD2L1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MAD2L1/Mad2 Monoclonal Antibody (M00785)
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