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Product Info Summary
| SKU: | PB9282 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-Mad2L1 Antibody Picoband®
SKU/Catalog Number
PB9282
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Mad2L1 Antibody Picoband® catalog # PB9282. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Mad2L1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9282)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Mad2L1 recombinant protein (Position: A2-D205). Human Mad2L1 shares 94% amino acid (aa) sequence identity with mouse Mad2L1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9282 is reactive to MAD2L1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
23 kDa
Calculated molecular weight
23510 MW
Background of MAD2L1
Mitotic spindle assembly checkpoint protein MAD2A is a protein that in humans is encoded by the MAD2L1 gene. This gene belongs to the MAD2 family. It is mapped to 4q27. MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. The protein has two highly different native conformations, an inactive open conformation that cannot bind CDC20 and that predominates in cytosolic monomers, and an active closed conformation. It is required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9282 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: 293T Whole Cell, COLO320 Whole Cell
ICC/IF: A431 cell
FCM: K562 cell
Validation Images & Assay Conditions
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Anti-Mad2L1 Picoband antibody, PB9282, Western blotting
All lanes: Anti Mad2L1 (PB9282) at 0.5ug/ml
Lane 1: 293T Whole Cell Lysate at 40ug
Lane 2: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 23KD
Observed bind size: 23KD
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Figure 2. IF analysis of Mad2L1 using anti-Mad2L1 antibody (PB9282).
Mad2L1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Mad2L1 Antibody (PB9282) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. Flow Cytometry analysis of K562 cells using anti-Mad2L1 antibody (PB9282).
Overlay histogram showing K562 cells stained with PB9282 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Mad2L1 Antibody (PB9282,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For MAD2L1 (Source: Uniprot.org, NCBI)
Gene Name
MAD2L1
Full Name
Mitotic spindle assembly checkpoint protein MAD2A
Weight
23510 MW
Superfamily
MAD2 family
Alternative Names
Mitotic spindle assembly checkpoint protein MAD2A;HsMAD2;Mitotic arrest deficient 2-like protein 1;MAD2-like protein 1;MAD2L1;MAD2; MAD2L1 HSMAD2, MAD2 mitotic arrest deficient 2 like 1 mitotic spindle assembly checkpoint protein MAD2A|MAD2 (mitotic arrest deficient, yeast, homolog)-like 1|MAD2 mitotic arrest deficient-like 1|MAD2-like protein 1|mitotic arrest deficient 2-like protein 1|mitotic arrest deficient, yeast, homolog-like 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAD2L1, check out the MAD2L1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAD2L1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Mad2L1 Antibody Picoband® (PB9282)
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Customer Q&As
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5 Customer Q&As for Anti-Mad2L1 Antibody Picoband®
Question
Please see the WB image, lot number and protocol we used for lung using anti-Mad2L1 antibody PB9282. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-21
Question
Would PB9282 anti-Mad2L1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
As indicated on the product datasheet, PB9282 anti-Mad2L1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-11
Question
We are currently using anti-Mad2L1 antibody PB9282 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2018-11-12
Answer
The anti-Mad2L1 antibody (PB9282) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-11-12
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-Mad2L1 antibody PB9282. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-04-05
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-04-05
Question
Do you have a BSA free version of anti-Mad2L1 antibody PB9282 available?
W. Jones
Verified customer
Asked: 2018-01-18
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Mad2L1 antibody PB9282 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-01-18
