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Product Info Summary
| SKU: | A00776-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-Bub1 Antibody Picoband®
SKU/Catalog Number
A00776-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Bub1 Antibody Picoband® catalog # A00776-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Bub1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00776-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Bub1 recombinant protein (Position: D40-K257).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00776-2 is reactive to Bub1 in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
122 kDa
Calculated molecular weight
122.375kDa
Background of BUB1
BUB1, also known as mitotic checkpoint serine/threonine kinase, is an enzyme that in humans is encoded by the BUB1 gene. It is mapped to 2q13. BUB1 is first identified in genetic screens of Saccharomyces cerevisiae. The protein is bound to kinetochores and plays a key role in the establishment of the mitotic spindle checkpoint and chromosome congression. The mitotic checkpoint kinase is evolutionary conserved in organisms as diverse as Saccharomyces cerevisiae and humans. Loss-of-function mutations or absence of BUB1 has been reported to result in aneuploidy, chromosomal instability (CIN) and premature senescence. The protein kinase BUB1 possesses versatile and distinct functions during the cell cycle, mainly in the SAC and chromosome alignment during metaphase.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00776-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Mouse
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: mouse testis tissue, rat heart tissue, rat PC-12 wholel cell, rat RH35 whole cell
FCM: HEPA1-6 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Bub1 using anti-Bub1 antibody (A00776-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse testis tissue lysates,
Lane 2: rat heart tissue lysates,
Lane 3: rat PC-12 wholel cell lysates,
Lane 4: rat RH35 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bub1 antigen affinity purified polyclonal antibody (Catalog # A00776-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bub1 at approximately 122KD. The expected band size for Bub1 is at 122KD.
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Figure 2. Flow Cytometry analysis of HEPA1-6 cells using anti-Bub1 antibody (A00776-2).
Overlay histogram showing HEPA1-6 cells stained with A00776-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Bub1 Antibody (A00776-2,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For Bub1 (Source: Uniprot.org, NCBI)
Gene Name
Bub1
Full Name
Mitotic checkpoint serine/threonine-protein kinase BUB1
Weight
122.375kDa
Superfamily
protein kinase superfamily
Alternative Names
RNA-binding protein FUS; 75 kDa DNA-pairing protein; Oncogene FUS; Oncogene TLS; POMp75; Translocated in liposarcoma protein; FUS; TLS; BUB1 BUB1AL, hBUB1, BUB1 BUB1 mitotic checkpoint serine/threonine kinase mitotic checkpoint serine/threonine-protein kinase BUB1|BUB1 budding uninhibited by benzimidazoles 1 homolog|budding uninhibited by benzimidazoles 1 homolog|mitotic spindle checkpoint kinase|putative serine/threonine-protein kinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Bub1, check out the Bub1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Bub1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Bub1 Antibody Picoband® (A00776-2)
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