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Product Info Summary
| SKU: | A00776 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-Bub1 Antibody
SKU/Catalog Number
A00776
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Bub1 Antibody (Catalog # A00776). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
Bub1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Bub1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00776)
Host
Rabbit
Contents
Bub1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Bub1 antibody was raised against a 19 amino acid synthetic peptide from near the amino terminus of human Bub1. The immunogen is located within amino acids 40 - 90 of Bub1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
SOX10 Antibody is predicted to not cross-react with other SOX familiy members.
Reactive Species
A00776 is reactive to BUB1 in Human, Mouse
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
122375 MW
Background of BUB1
Bub1 was initially identified as the mammalian homolog to the yeast protein through a cDNA screen. Bub1 encodes a kinase involved in the formation of the spindle checkpoint, an important mechanism that ensures high fidelity mitotic chromosome segregation. It is thought to be required for assembly of a functional inner centromere, sister chromatid cohesion via targeting of the Shugoshim protein and metaphase congression. Bub1 functions by phosphorylating cdc20, a member of the mitotic checkpoint complex and activating the spindle checkpoint. A related protein kinase Bub3 interacts with Bub1 and targets it to kinetochores prior to chromosome alignment. Mutations in bub1 have been associated with aneuploidy and several forms of cancer.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00776 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Bub1 antibody can be used for detection of bub1 by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of bub1 in A-20 lysate with bub1 antibody at (A) 2 and (B) 4 μg/mL.
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Immunocytochemistry of Bub1 in A20 cells with Bub1 antibody at 10 μg/mL.
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Immunofluorescence of Bub1 in A20 cells with Bub1 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For BUB1 (Source: Uniprot.org, NCBI)
Gene Name
BUB1
Full Name
Mitotic checkpoint serine/threonine-protein kinase BUB1
Weight
122375 MW
Superfamily
protein kinase superfamily
Alternative Names
BUB1A, BUB1L, hBUB1, BUB1A BUB1 BUB1AL, hBUB1, BUB1 BUB1 mitotic checkpoint serine/threonine kinase mitotic checkpoint serine/threonine-protein kinase BUB1|BUB1 budding uninhibited by benzimidazoles 1 homolog|budding uninhibited by benzimidazoles 1 homolog|mitotic spindle checkpoint kinase|putative serine/threonine-protein kinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BUB1, check out the BUB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BUB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Bub1 Antibody (A00776)
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