Anti-Integrin alpha 5/ITGA5 Antibody Picoband™

Product Name

Anti-Integrin alpha 5/ITGA5 Antibody Picoband™

View all ITGA5 Antibodies

SKU/Catalog Number

PB9254

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Integrin alpha 5/ITGA5 Antibody Picoband™ catalog # PB9254. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Integrin alpha 5/ITGA5 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9254)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Integrin alpha 5 recombinant protein (Position: E787-E992). Human Integrin alpha 5 shares 86% amino acid (aa) sequence identity with mouse Integrin alpha 5.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9254 is reactive to ITGA5 in Human, Mouse, Rat

Applications

PB9254 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

130 kDa

Calculated molecular weight

114536 MW

Background of ITGA5

Integrin alpha-5, also known as FNRA or VLA5A, is a protein that in humans is encoded by the ITGA5 gene. The product of this gene belongs to the integrin alpha chain family. Integrins are integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of ITGA5 using anti-ITGA5 antibody (PB9254).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: rat liver tissue lysates,
Lane 3: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITGA5 antigen affinity purified polyclonal antibody (Catalog # PB9254) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ITGA5 at approximately 130 kDa. The expected band size for ITGA5 is at 115 kDa.

Gene/Protein Information For ITGA5 (Source: Uniprot.org, NCBI)

Alternative Names

Integrin alpha 5 beta 1; VLA-5 ITGA5 CD49e, FNRA, VLA-5, VLA5A integrin subunit alpha 5 integrin alpha-5|CD49 antigen-like family member E|ITGA5/SLC9B1 fusion|fibronectin receptor subunit alpha|fibronectin receptor, alpha polypeptide|fibronectin receptor, alpha subunit|integrin alpha-F|integrin, alpha 5 (fibronectin receptor, alpha polypeptide)|very late activation protein 5, alpha subunit

For more info on ITGA5, check out the ITGA5 Infographic

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In this infographic, you will see the following information for ITGA5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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