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Product Info Summary
| SKU: | A00013-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-HIF-1 alpha/HIF1A Antibody Picoband®
View all HIF-1 alpha Antibodies
SKU/Catalog Number
A00013-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-HIF-1 alpha/HIF1A Antibody Picoband® catalog # A00013-1. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HIF-1 alpha/HIF1A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00013-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human HIF-1 alpha/HIF1A recombinant protein (Position: H197-R718).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00013-1 is reactive to HIF1A in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
123 kDa
Calculated molecular weight
137910 MW
Background of HIF-1 alpha
HIF-1α (Hypoxia-inducible factor 1α,HIF1A) is a transcription factor that mediates cellular and systemic homeostatic responses to reduced O2 availability in mammals, including angiogenesis, erythropoiesis and glycolysis. This gene was mapped to 14q21-q24. HIF-1α transactivate genes required for energy metabolism and tissue perfusion and is necessary for embryonic development and tumor explant growth. HIF-1alpha is over expressed during carcinogenesis, myocardial infarction and wound healing. It is crucial for the cellular response to hypoxia and is frequently over expressed in human cancers, resulting in the activation of genes essential for cell survival. HIF-1α regulates the survival and function in the inflammatory microenvironment directly. It is a transcription factor that plays a pivotal role in cellular adaptation to changes in oxygen availability.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00013-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human U-87MG whole cell, human U2OS whole cell, human PC-3 whole cell, human A549 whole cell, human HepG2 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of HIF1A using anti-HIF1A antibody (A00013-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human U-87MG whole cell lysates,
Lane 2: human U2OS whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: human HepG2 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIF1A antigen affinity purified polyclonal antibody (Catalog # A00013-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIF1A at approximately 123KD. The expected band size for HIF1A is at 93KD.
Protein Target Info & Infographic
Gene/Protein Information For HIF1A (Source: Uniprot.org, NCBI)
Gene Name
HIF1A
Full Name
Hypoxia-inducible factor 1-alpha
Weight
137910 MW
Alternative Names
Hypoxia-inducible factor 1-alpha; HIF-1-alpha; HIF1-alpha; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78; bHLHe78; PAS domain-containing protein 8; HIF1A; BHLHE78, MOP1 HIF1A HIF-1-alpha, HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78 hypoxia inducible factor 1 subunit alpha hypoxia-inducible factor 1-alpha|ARNT interacting protein|PAS domain-containing protein 8|basic-helix-loop-helix-PAS protein MOP1|class E basic helix-loop-helix protein 78|hypoxia inducible factor 1 alpha subunit|hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)|hypoxia-inducible factor1alpha|member of PAS protein 1|member of PAS superfamily 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HIF1A, check out the HIF1A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HIF1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HIF-1 alpha/HIF1A Antibody Picoband® (A00013-1)
Hello CJ!
A00013-1 has been cited in 11 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
p53 isoform Δ113p53 promotes zebrafish heart regeneration by maintaining redox homeostasis
Qu KS, Luo Y, Yan XN, Kuai L, Ru Y, Yue Luo, Song JK, Ji WL, Li B, Xing M. Qinzhuliangxue mixture alleviates psoriasis-like skin lesions via inhibiting the IL6/STAT3 axis. J Ethnopharmacol. 2021 Mar 20:114041. doi: 10.1016/j.jep.2021.114041. Epub ahead of print. PMID: 33757812.
Species: Mouse
A00013-1 usage in article: APP:IHC, SAMPLE:SKIN TISSUE, DILUTION:1:900
Ye S,Zhao T,Zhang W,Tang Z,Gao C,Ma Z,Xiong JW,Peng J,Tan WQ,Chen J.p53 isoform Δ113p53 promotes zebrafish heart regeneration by maintaining redox homeostasis.Cell Death Dis.2020 Jul 23;11(7):568.doi:10.1038/s41419-020-02781-7.PMID:32703938;PMCID:PMC73782
Species: Zebrafish
A00013-1 usage in article: APP:WB, SAMPLE:HEART TISSUE, DILUTION:NA
The zebrafish miR-125c is induced under hypoxic stress via hypoxia-inducible factor 1%u03B1 and functions in cellular adaptations and embryogenesis
Downregulating hypoxia%u2010inducible factor%u20102%u03B1 improves the efficacy of doxorubicin in the treatment of hepatocellular carcinoma
Antisense hypoxia%u2010inducible factor 1%u03B1 gene therapy enhances the therapeutic efficacy of doxorubicin to combat hepatocellular carcinoma
The Expression of FAP in Hepatocellular Carcinoma Cells is Induced by Hypoxia and Correlates with Poor Clinical Outcomes
2-Methoxyestradiol inhibits the proliferation and migration and reduces the radioresistance of nasopharyngeal carcinoma CNE-2 stem cells via NF-%u03BAB/HIF-1 %u2026
Effects of acute hypoxia and reoxygenation on physiological and immune responses and redox balance of Wuchang bream (Megalobrama amblycephala Yih %u2026
Targeting hexokinase 2 inhibition promotes radiosensitization in HPV16 E7-induced cervical cancer and suppresses tumor growth
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-HIF-1 alpha/HIF1A Antibody Picoband®
Question
Is a blocking peptide available for product anti-HIF-1 alpha/HIF1A antibody (A00013-1)?
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
We do provide the blocking peptide for product anti-HIF-1 alpha/HIF1A antibody (A00013-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-04-23
Question
We purchased anti-HIF-1 alpha/HIF1A antibody for ELISA on hepatoma in the past. I am using human, and I plan to use the antibody for WB next. I am looking for examining hepatoma as well as visceral pleura in our next experiment. Could give a recommendation on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2020-02-19
Answer
I looked at the website and datasheets of our anti-HIF-1 alpha/HIF1A antibody and it appears that A00013-1 has been tested on human in both ELISA and WB. Thus A00013-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-02-19
Question
Would anti-HIF-1 alpha/HIF1A antibody A00013-1 work for WB with glial tumor?
Verified Customer
Verified customer
Asked: 2020-02-14
Answer
According to the expression profile of glial tumor, HIF1A is highly expressed in glial tumor. So, it is likely that anti-HIF-1 alpha/HIF1A antibody A00013-1 will work for WB with glial tumor.
Boster Scientific Support
Answered: 2020-02-14
Question
We were content with the WB result of your anti-HIF-1 alpha/HIF1A antibody. However we have seen positive staining in visceral pleura cytoplasm. using this antibody. Is that expected? Could you tell me where is HIF1A supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-12-12
Answer
Based on literature, visceral pleura does express HIF1A. Generally HIF1A expresses in cytoplasm. Regarding which tissues have HIF1A expression, here are a few articles citing expression in various tissues:
Choriocarcinoma, and Placenta, Pubmed ID: 15489334
Hepatoma, Pubmed ID: 9079689
Liver, Pubmed ID: 12508121
Boster Scientific Support
Answered: 2019-12-12
Question
I was wanting to use your anti-HIF-1 alpha/HIF1A antibody for WB for human glial tumor on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human glial tumor identification?
Verified Customer
Verified customer
Asked: 2019-12-05
Answer
It shows on the product datasheet, A00013-1 anti-HIF-1 alpha/HIF1A antibody has been validated for ELISA, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human glial tumor in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-05
Question
Will A00013-1 anti-HIF-1 alpha/HIF1A antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-09
Answer
You can see on the product datasheet, A00013-1 anti-HIF-1 alpha/HIF1A antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-09
Question
Is this A00013-1 anti-HIF-1 alpha/HIF1A antibody reactive to the isotypes of HIF1A?
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
The immunogen of A00013-1 anti-HIF-1 alpha/HIF1A antibody is E.coli-derived human HIF-1 alpha/HIF1A recombinant protein (Position: H197-R718). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-29
Question
We are currently using anti-HIF-1 alpha/HIF1A antibody A00013-1 for human tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2018-11-27
Answer
The anti-HIF-1 alpha/HIF1A antibody (A00013-1) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-11-27
Question
I was wanting to use to test anti-HIF-1 alpha/HIF1A antibody A00013-1 on human glial tumor for research purposes, then I may be interested in using anti-HIF-1 alpha/HIF1A antibody A00013-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-09-14
Answer
The products we sell, including anti-HIF-1 alpha/HIF1A antibody A00013-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-09-14
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for glial tumor using anti-HIF-1 alpha/HIF1A antibody A00013-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-06-29
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-06-29
Question
We need using your anti-HIF-1 alpha/HIF1A antibody for positive regulation of erythrocyte differentiation studies. Has this antibody been tested with western blotting on a549 whole cell lysates? We would like to see some validation images before ordering.
D. Jha
Verified customer
Asked: 2017-12-26
Answer
Thanks for your inquiry. This A00013-1 anti-HIF-1 alpha/HIF1A antibody is validated on human a549, u2os whole cell lysates, a549 whole cell lysates, hepg2 whole cell lysates. It is guaranteed to work for ELISA, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-12-26
Question
Here is the WB image, lot number and protocol we used for glial tumor using anti-HIF-1 alpha/HIF1A antibody A00013-1. Please let me know if you require anything else.
N. Krishna
Verified customer
Asked: 2017-09-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-09-27
Question
We have seen staining in human liver. What should we do? Is anti-HIF-1 alpha/HIF1A antibody supposed to stain liver positively?
J. Jha
Verified customer
Asked: 2014-12-19
Answer
From what I have seen in literature liver does express HIF1A. From what I have seen in Uniprot.org, HIF1A is expressed in visceral pleura, hepatoma, glial tumor, liver, choriocarcinoma placenta, among other tissues. Regarding which tissues have HIF1A expression, here are a few articles citing expression in various tissues:
Choriocarcinoma, and Placenta, Pubmed ID: 15489334
Hepatoma, Pubmed ID: 9079689
Liver, Pubmed ID: 12508121
Boster Scientific Support
Answered: 2014-12-19
Question
I see that the anti-HIF-1 alpha/HIF1A antibody A00013-1 works with WB, what is the protocol used to produce the result images on the product page?
J. Zhao
Verified customer
Asked: 2014-03-13
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2014-03-13
Question
Can you help my question with product A00013-1, anti-HIF-1 alpha/HIF1A antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
B. Williams
Verified customer
Asked: 2013-03-18
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00013-1 anti-HIF-1 alpha/HIF1A antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-03-18
Question
Is there a BSA free version of anti-HIF-1 alpha/HIF1A antibody A00013-1 available?
J. Anderson
Verified customer
Asked: 2013-02-22
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-HIF-1 alpha/HIF1A antibody A00013-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-02-22
