Product Info Summary
SKU: | PB9129 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-HIF1 beta/ARNT Antibody Picoband®
View all ARNT/HIF-1 beta Antibodies
SKU/Catalog Number
PB9129
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-HIF1 beta/ARNT Antibody Picoband® catalog # PB9129. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HIF1 beta/ARNT Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9129)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human HIF1 beta recombinant protein (Position: V416-E789). Human HIF1 beta shares 86% and 83% amino acid (aa) sequences identity with mouse and rat HIF1 beta, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9129 is reactive to HIF1A in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
87 kDa
Calculated molecular weight
86636 MW
Background of ARNT/HIF-1 beta
ARNT is also known as HIF1-beta or HIF1B. This gene encodes a protein containing a basic helix-loop-helix domain and two characteristic PAS domains along with a PAC domain. It is mapped to 1q21.3. The encoded protein binds to ligand-bound aryl hydrocarbon receptor and aids in the movement of this complex to the nucleus, where it promotes the expression of genes involved in xenobiotic metabolism. This protein is also a co-factor for transcriptional regulation by hypoxia-inducible factor 1. ARNT is a structural component of the XRE-binding form of the Ah receptor. It also functions in concert with RelB in a CD30-induced negative feedback mechanism.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9129 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human Hela whole cell, human 293T whole cell, human Jurkat whole cell, human U87 whole cell, human COLO320 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of HIF1 using anti-HIF1 antibody (PB9129).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant human HIF1 beta protein 0.5 ng.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIF1 antigen affinity purified polyclonal antibody (Catalog # PB9129) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIF1 at approximately 49 kDa. The expected band size for HIF1 is at 49 kDa.
Click image to see more details
Figure 2. Western blot analysis of HIF1 using anti-HIF1 antibody (PB9129).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human U87 whole cell lysates,
Lane 5: human COLO320 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIF1 antigen affinity purified polyclonal antibody (Catalog # PB9129) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIF1 at approximately 87 kDa. The expected band size for HIF1 is at 87 kDa.
Protein Target Info & Infographic
Gene/Protein Information For HIF1A (Source: Uniprot.org, NCBI)
Gene Name
HIF1A
Full Name
Aryl hydrocarbon receptor nuclear translocator
Weight
86636 MW
Alternative Names
Aryl hydrocarbon receptor nuclear translocator;ARNT protein;Class E basic helix-loop-helix protein 2;bHLHe2;Dioxin receptor, nuclear translocator;Hypoxia-inducible factor 1-beta;HIF-1-beta;HIF1-beta;ARNT;BHLHE2; ARNT HIF-1-beta, HIF-1beta, HIF1-beta, HIF1B, HIF1BETA, TANGO, bHLHe2 aryl hydrocarbon receptor nuclear translocator aryl hydrocarbon receptor nuclear translocator|class E basic helix-loop-helix protein 2|dioxin receptor, nuclear translocator|hypoxia-inducible factor 1, beta subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HIF1A, check out the HIF1A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HIF1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HIF1 beta/ARNT Antibody Picoband® (PB9129)
Hello CJ!
PB9129 has been cited in 28 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
TLR4 promotes the expression of HIF-1? by triggering reactive oxygen species in cervical cancer cells in vitro-implications for therapeutic intervention
Targeting hexokinase 2 inhibition promotes radiosensitization in HPV16 E7-induced cervical cancer and suppresses tumor growth
Suppression of the expression of hypoxia-inducible factor-1? by RNA interference alleviates hypoxia-induced pulmonary hypertension in adult rats
Effect of rapamycin on endometriosis in mice
Hypoxic preconditioning increases the protective effect of bone marrow mesenchymal stem cells on spinal cord ischemia/reperfusion injury
Local recurrence of small cell lung cancer following radiofrequency ablation is induced by HIF-1? expression in the transition zone
Effect of Hyperbaric Oxygen on the Growth of Intracranial Glioma in Rats
Increased hypoxia-inducible factor 1alpha expression in rat brain tissues in response to aging
Semaphorin 4D and hypoxia-inducible factor-1? overexpression is related to prognosis in colorectal carcinoma
Evidence for a therapeutic effect of Braintone on ischemic brain damage
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Customer Q&As
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1 Customer Q&As for Anti-HIF1 beta/ARNT Antibody Picoband®
Question
We are currently using anti-HIF1 beta/ARNT antibody PB9129 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on goat tissues as well?
R. Evans
Verified customer
Asked: 2019-12-18
Answer
The anti-HIF1 beta/ARNT antibody (PB9129) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-18