Anti-Ms CD49e Purified Itga5 Monoclonal Antibody

Product Name

Anti-Ms CD49e Purified Itga5 Monoclonal Antibody

View all ITGA5 Antibodies

SKU/Catalog Number

M01911-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Ms CD49e Purified Itga5 Monoclonal Antibody (Catalog# M01911-1). Tested in ICC, Flow Cytometry, IHC-F application(s). This antibody reacts with Mouse.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-Ms CD49e Purified Itga5 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01911-1)

Host

Rat

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

5H10-27 (MFR5)

Isotype

Rat IgG2a kappa

Immunogen

C57BL/6 x A/J)F1 murine mast cell line. The rat monoclonal antibody 5H10-27 (MFR5) recognizes an extracellular epitope of murine CD49e, a 135 kDa protein serving as VLA-5 alpha chain, expressed on thymocytes, activated T cells, splenic B cells, and mast cells.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01911-1 is reactive to Itga5 in Mouse

Reconstitution

Observed Molecular Weight

19 kDa

Calculated molecular weight

115043 MW

Background of ITGA5

CD49e, the alpha 5 integrin, noncovalently associates with the beta 1integrin (CD29) to form VLA-5 integrin complex. CD49e itself is composed of two disulfide-linked chains of 135 kDa and 25 kDa. VLA-5 binds to RGD sequence of fibronectin, and to neural adhesion molecule L1. It is important in maintaining the integrity of endothelial monolayers, in monocyte migration into the extracellular tissues, and it also provides a co-stimulatory signal to T cells and enhances receptor and complement receptor-mediated phagocytosis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M01911-1 is guaranteed for Flow Cytometry, IHC-F, ICC Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow cytometry: 1-4 μg/ml.

Validation Images & Assay Conditions

There are currently no images for this product. We are working on uploading them please check back shortly or contact us to expedite our upload process for this antibody.

Gene/Protein Information For Itga5 (Source: Uniprot.org, NCBI)

Alternative Names

ITGA5, VLA-5 alpha, FNRA ITGA5 CD49e, FNRA, VLA-5, VLA5A integrin subunit alpha 5 integrin alpha-5|CD49 -like family member E|ITGA5/SLC9B1 fusion|fibronectin receptor subunit alpha|fibronectin receptor, alpha polypeptide|fibronectin receptor, alpha subunit|integrin alpha-F|integrin, alpha 5 (fibronectin receptor, alpha polypeptide)|very late activation protein 5, alpha subunit

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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