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Product Info Summary
| SKU: | PA2036-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-IKK beta/IKBKB Antibody Picoband®
SKU/Catalog Number
PA2036-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IKK beta/IKBKB Antibody catalog # PA2036-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IKK beta/IKBKB Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2036-1)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human IKK beta, identical to the related mouse sequence, and different from the related rat sequence by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2036-1 is reactive to IKBKB in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
87 kDa
Calculated molecular weight
86564 MW
Background of IKK beta
IKBKB (Inhibitor of Kappa Light Chain Gene Enhancer in B Cells, Kinase of, Beta), also known as IKKB or NFKBIKB , is a protein that in humans is encoded by the IKBKB gene. Shindo et al. (1998) mapped the IKBKB gene to chromosome 8p12-p11 by FISH. Mercurio et al. (1997) found that mutations in IKK2 had a more pronounced effect upon NFKB activation than did comparable mutations in IKK1. Yin et al. (1998) tested the activity of various antiinflammatory agents on the IKK complex. They demonstrated that aspirin and sodium salicylate specifically inhibit IKK-beta activity in vitro and in vivo by binding to IKK-beta to reduce ATP binding.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2036-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: Rat Cardiac Muscle Tissue, Rat Skeletal Muscle Tissue, PANC Whole Cell, MCF-7Whole Cell, HEPG2 Whole Cell, COLO320 Cell
Validation Images & Assay Conditions
Click image to see more details
Anti-IKBKB antibody, PA2036-1, Western blotting
All lanes: Anti IKBKB (PA2036-1) at 0.5ug/ml
Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 2: Rat Skeletal Muscle Tissue Lysate at 50ug
Lane 3: PANC Whole Cell Lysate at 40ug
Lane 4: MCF-7Whole Cell Lysate at 40ug
Lane 5: HEPG2 Whole Cell Lysate at 40ug
Lane 6: COLO320 Cell Lysate at 40ug
Predicted bind size: 87KD
Observed bind size: 87KD
Protein Target Info & Infographic
Gene/Protein Information For IKBKB (Source: Uniprot.org, NCBI)
Gene Name
IKBKB
Full Name
Inhibitor of nuclear factor kappa-B kinase subunit beta
Weight
86564 MW
Superfamily
protein kinase superfamily
Alternative Names
Inhibitor of nuclear factor kappa-B kinase subunit beta;I-kappa-B-kinase beta;IKK-B;IKK-beta;IkBKB;2.7.11.10;I-kappa-B kinase 2;IKK2;Nuclear factor NF-kappa-B inhibitor kinase beta;NFKBIKB;IKBKB;IKKB; IKBKB IKK-beta, IKK2, IKKB, IMD15, IMD15A, IMD15B, NFKBIKB inhibitor of nuclear factor kappa B kinase subunit beta inhibitor of nuclear factor kappa-B kinase subunit beta|I-kappa-B kinase 2|I-kappa-B-kinase beta|inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta|nuclear factor NF-kappa-B inhibitor kinase beta|serine/threonine protein kinase IKBKB
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IKBKB, check out the IKBKB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IKBKB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IKK beta/IKBKB Antibody Picoband® (PA2036-1)
Hello CJ!
PA2036-1 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Gastro-Protective Effects of Albizia anthelmintica Leaf Extract on Indomethacin-Induced Gastric Ulcer in Wistar Rats: In Silico and In Vivo Studies
Prolonged overnutrition with fructose or fat induces metabolic derangements in rats by disrupting the crosstalk between the hypothalamus and periphery: Possible amelioration with fenofibrate
Nabil M, El Raey MA, Abdo W, Abdelfattah MAO, El-Shazly AM, Sobeh M, Mahmoud MF. Gastro-Protective Effects of Albizia anthelmintica Leaf Extract on Indomethacin-Induced Gastric Ulcer in Wistar Rats: In Silico and In Vivo Studies. Antioxidants (Basel). 202
Species: Rat
PA2036-1 usage in article: APP:WB, SAMPLE:GASTRIC TISSUE, DILUTION:1:1000
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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7 Customer Q&As for Anti-IKK beta/IKBKB Antibody Picoband®
Question
I am interested in to test anti-IKK beta/IKBKB antibody PA2036-1 on mouse leukemic t-cell for research purposes, then I may be interested in using anti-IKK beta/IKBKB antibody PA2036-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
G. Thomas
Verified customer
Asked: 2020-04-21
Answer
The products we sell, including anti-IKK beta/IKBKB antibody PA2036-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-04-21
Question
Can you help my question with product PA2036-1, anti-IKK beta/IKBKB antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-07
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2036-1 anti-IKK beta/IKBKB antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-07
Question
Is there a BSA free version of anti-IKK beta/IKBKB antibody PA2036-1 available?
Verified Customer
Verified customer
Asked: 2019-12-04
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-IKK beta/IKBKB antibody PA2036-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-04
Question
I see that the anti-IKK beta/IKBKB antibody PA2036-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-01
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-08-01
Question
Would anti-IKK beta/IKBKB antibody PA2036-1 work for WB with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2019-06-24
Answer
According to the expression profile of leukemic t-cell, IKBKB is highly expressed in leukemic t-cell. So, it is likely that anti-IKK beta/IKBKB antibody PA2036-1 will work for WB with leukemic t-cell.
Boster Scientific Support
Answered: 2019-06-24
Question
Will PA2036-1 anti-IKK beta/IKBKB antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-11-29
Answer
You can see on the product datasheet, PA2036-1 anti-IKK beta/IKBKB antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-11-29
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-IKK beta/IKBKB antibody PA2036-1. Let me know if you need anything else.
C. Jha
Verified customer
Asked: 2016-05-24
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-05-24
