Anti-PHD2 / prolyl hydroxylase Monoclonal Antibody

Product Name

Anti-PHD2 / prolyl hydroxylase Monoclonal Antibody

View all EGLN1/PHD2 Antibodies

SKU/Catalog Number

M00415

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-PHD2 / prolyl hydroxylase Monoclonal Antibody catalog # M00415. Tested in WB, IP applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PHD2 / prolyl hydroxylase Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00415)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

ACEF-5

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human PHD2 / prolyl hydroxylase Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00415 is reactive to EGLN1 in Human

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Observed Molecular Weight

52 kDa

Calculated molecular weight

10835 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00415 is guaranteed for IP, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IP 1:50

Validation Images & Assay Conditions

Gene/Protein Information For EGLN1 (Source: Uniprot.org, NCBI)

Alternative Names

Protein S100-A8;Calgranulin-A;Calprotectin L1L subunit;Cystic fibrosis antigen;CFAG;Leukocyte L1 complex light chain;Migration inhibitory factor-related protein 8;MRP-8;p8;S100 calcium-binding protein A8;Urinary stone protein band A;Protein S100-A8, N-terminally processed;S100A8;CAGA, CFAG, MRP8; EGLN1 C1orf12, ECYT3, HALAH, HIF-PH2, HIFPH2, HPH-2, HPH2, PHD2, SM20, ZMYND6 egl-9 family hypoxia inducible factor 1 egl nine homolog 1|HIF-prolyl hydroxylase 2|egl nine-like protein 1|hypoxia-inducible factor prolyl hydroxylase 2|prolyl hydroxylase domain-containing protein 2|zinc finger MYND domain-containing protein 6

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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