Click image to see more details
Product Info Summary
| SKU: | M00144 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-CD11b ITGAM Rabbit Monoclonal Antibody
SKU/Catalog Number
M00144
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CD11b ITGAM Rabbit Monoclonal Antibody catalog # M00144. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD11b ITGAM Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00144)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
BCG-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CD11b
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00144 is reactive to ITGAM in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
95 kDa
Calculated molecular weight
127179 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00144 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Positive Control
WB: TF1 cell
IHC: human colon carcinoma tissue
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of CD11b expression in TF1 cell lysate.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using CD11b Antibody.
Protein Target Info & Infographic
Gene/Protein Information For ITGAM (Source: Uniprot.org, NCBI)
Gene Name
ITGAM
Full Name
Integrin alpha-M
Weight
127179 MW
Superfamily
integrin alpha chain family
Alternative Names
Integrin alpha-M;CD11 antigen-like family member B;CR-3 alpha chain;Cell surface glycoprotein MAC-1 subunit alpha;Leukocyte adhesion receptor MO1;Neutrophil adherence receptor;CD11b;ITGAM;CD11B, CR3A; ITGAM CD11B, CR3A, MAC-1, MAC1A, MO1A, SLEB6 integrin subunit alpha M integrin alpha-M|CD11 -like family member B|CR-3 alpha chain| CD11b (p170)|cell surface glycoprotein MAC-1 subunit alpha|complement component 3 receptor 3 subunit|integrin, alpha M (complement component 3 receptor 3 subunit)|leukocyte adhesion receptor MO1|macrophage alpha polypeptide|neutrophil adherence receptor alpha-M subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ITGAM, check out the ITGAM Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ITGAM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD11b ITGAM Rabbit Monoclonal Antibody (M00144)
Hello CJ!
No publications found for M00144
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-CD11b ITGAM Rabbit Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-CD11b ITGAM Rabbit Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
4 Customer Q&As for Anti-CD11b ITGAM Rabbit Monoclonal Antibody
Question
Our team were well pleased with the WB result of your anti-CD11b Rabbit Monoclonal antibody. However we have seen positive staining in blood cell membrane using this antibody. Is that expected? Could you tell me where is ITGAM supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
According to literature, blood does express ITGAM. Generally ITGAM expresses in cell membrane. Regarding which tissues have ITGAM expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 1346576, 3539202
Liver, Pubmed ID: 19159218
Boster Scientific Support
Answered: 2019-06-28
Question
Our lab used your anti-CD11b Rabbit Monoclonal antibody for WB on bone marrow cell in the past. I am using human, and We intend to use the antibody for ICC next. My question regards examining bone marrow cell as well as liver in our next experiment. Do you have any suggestion on which antibody would work the best for ICC?
Verified Customer
Verified customer
Asked: 2017-11-20
Answer
I took a look at the website and datasheets of our anti-CD11b Rabbit Monoclonal antibody and it seems that M00144 has been tested on human in both WB and ICC. Thus M00144 should work for your application. Our Boster satisfaction guarantee will cover this product for ICC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ICC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-11-20
Question
We are currently using anti-CD11b Rabbit Monoclonal antibody M00144 for human tissue, and we are content with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on horse tissues as well?
L. Johnson
Verified customer
Asked: 2017-06-20
Answer
The anti-CD11b Rabbit Monoclonal antibody (M00144) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-06-20
Question
We have seen staining in human blood. Do you have any suggestions? Is anti-CD11b Rabbit Monoclonal antibody supposed to stain blood positively?
M. Zhao
Verified customer
Asked: 2014-01-31
Answer
From what I have seen in literature blood does express ITGAM. From what I have seen in Uniprot.org, ITGAM is expressed in bone marrow cell, blood, liver, among other tissues. Regarding which tissues have ITGAM expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 1346576, 3539202
Liver, Pubmed ID: 19159218
Boster Scientific Support
Answered: 2014-01-31
