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Product Info Summary
| SKU: | A00464 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-COMT Antibody Picoband®
SKU/Catalog Number
A00464
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-COMT Antibody Picoband® catalog # A00464. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-COMT Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00464)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse COMT recombinant protein (Position: R20-S265).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00464 is reactive to Comt in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
25 kDa, 29 kDa
Calculated molecular weight
30.037kDa
Background of COMT
Catechol O-methyltransferase, also called COMT, is one of the major mammalian enzymes involved in the metabolic degradation of catecholamines. This gene is mapped to 22q11.21. Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00464 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: mouse liver tissue, mouse stomach tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of COMT using anti-COMT antibody (A00464).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse liver tissue lysates,
Lane 2: mouse stomach tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COMT antigen affinity purified polyclonal antibody (Catalog # A00464) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for COMT at approximately 25 and 29KD. The expected band size for COMT is at 29KD.
Protein Target Info & Infographic
Gene/Protein Information For Comt (Source: Uniprot.org, NCBI)
Gene Name
Comt
Full Name
Catechol O-methyltransferase
Weight
30.037kDa
Superfamily
class I-like SAM-binding methyltransferase superfamily
Alternative Names
Catechol O-methyltransferase; Comt; Comt1 COMT HEL-S-98n catechol-O-methyltransferase catechol O-methyltransferase|epididymis secretory sperm binding protein Li 98n|testicular tissue protein Li 42
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Comt, check out the Comt Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Comt: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-COMT Antibody Picoband® (A00464)
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No publications found for A00464
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Customer Q&As
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5 Customer Q&As for Anti-COMT Antibody Picoband®
Question
I see that the anti-COMT antibody A00464 works with ELISA, what is the protocol used to produce the result images on the product page?
A. Mangal
Verified customer
Asked: 2020-04-23
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-04-23
Question
I have a question about product A00464, anti-COMT antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-18
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00464 anti-COMT antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-18
Question
Is a blocking peptide available for product anti-COMT antibody (A00464)?
Verified Customer
Verified customer
Asked: 2019-10-17
Answer
We do provide the blocking peptide for product anti-COMT antibody (A00464). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-10-17
Question
Will A00464 anti-COMT antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
J. Li
Verified customer
Asked: 2018-05-15
Answer
It shows on the product datasheet, A00464 anti-COMT antibody as been validated on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-05-15
Question
Does anti-COMT antibody A00464 work for ELISA with placenta?
R. Kulkarni
Verified customer
Asked: 2016-02-11
Answer
According to the expression profile of placenta, COMT is highly expressed in placenta. So, it is likely that anti-COMT antibody A00464 will work for ELISA with placenta.
Boster Scientific Support
Answered: 2016-02-11
