Anti-COMT Antibody Picoband® (monoclonal, 15C10)

COMT antibody

Boster Bio Anti-COMT Antibody Picoband® (monoclonal, 15C10) catalog # M00464. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: M00464
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Mouse
Application: Flow Cytometry, IF, IHC, ICC, WB

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Product Name

Anti-COMT Antibody Picoband® (monoclonal, 15C10)

View all COMT Antibodies

SKU/Catalog Number

M00464

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-COMT Antibody Picoband® (monoclonal, 15C10) catalog # M00464. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-COMT Antibody Picoband® (monoclonal, 15C10) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00464)

Host

Mouse

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Monoclonal

Clone Number

15C10

Isotype

Mouse IgG2b

Immunogen

E.coli-derived human COMT recombinant protein (Position: G52-P271). Human COMT shares 81.9% and 81% amino acid (aa) sequence identity with mouse and rat COMT, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

M00464 is reactive to COMT in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Observed Molecular Weight

29 kDa

Calculated molecular weight

30.037kDa

Background of COMT

Catechol O-methyltransferase, also called COMT, is one of the major mammalian enzymes involved in the metabolic degradation of catecholamines. This gene is mapped to 22q11.21. Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00464 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human,Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human

Positive Control

WB: human HepG2 whole cell, human K562 whole cell, human THP-1 whole cell, human HEK293 whole cell, human A549 whole cell, human Caco-2 whole cell, rat RH35 whole cell, mouse Neuro-2a whole cell,
IHC: human intestinal cancer tissue, human mammary cancer tissue, human mammary cancer tissue, human placenta tissue, human lung cancer tissue, mouse testis tissue, rat testis tissue
ICC/IF: A431 cell, MCF7 cell
FCM: K562 cell, U87 cell

Validation Images & Assay Conditions

Gene/Protein Information For COMT (Source: Uniprot.org, NCBI)

Gene Name

COMT

Full Name

Catechol O-methyltransferase

Weight

30.037kDa

Superfamily

class I-like SAM-binding methyltransferase superfamily

Alternative Names

Catechol O-methyltransferase; COMT COMT HEL-S-98n catechol-O-methyltransferase catechol O-methyltransferase|epididymis secretory sperm binding protein Li 98n|testicular tissue protein Li 42

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on COMT, check out the COMT Infographic

COMT infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for COMT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
M00464

100μg

$370
M00464-10ug

10μg sample (liquid)

$99
M00464-Biotin

100 μg Biotin conjugated

$570
M00464-Cy3

100 μg Cy3 conjugated

$570
M00464-Dylight488

100 μg Dylight488 conjugated

$570
M00464-Dylight550

100 μg Dylight550 conjugated

$570
M00464-Dylight594

100 μg Dylight594 conjugated

$570
M00464-FITC

100 μg FITC conjugated

$570
M00464-HRP

100 μg HRP conjugated

$570
M00464-APC

100 μg APC conjugated

$670
M00464-PE

100 μg PE conjugated

$670
M00464-iFluor647

100 μg iFluor647 conjugated

$670
M00464-carrier-free

Carrier Free

$370

More conjugates/formats

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M00464
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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