Click image to see more details
Product Info Summary
| SKU: | M00377-Dyl488 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Human CD46 DyLight® 488 conjugated Antibody(monoclonal, 9E9)
SKU/Catalog Number
M00377-Dyl488
Size
100 μg/vial
Form
Liquid
Description
Boster Bio Anti-Human CD46 DyLight® 488 conjugated Antibody (monoclonal, 9E9) catalog # M00377-Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Cite This Product
Anti-Human CD46 DyLight® 488 conjugated Antibody(monoclonal, 9E9) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00377-Dyl488)
Host
Mouse
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN5.
Clonality
Monoclonal
Clone Number
9E9
Isotype
Mouse IgG1
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human CD46.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M00377-Dyl488 is reactive to CD46 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Observed Molecular Weight
39 kDa
Calculated molecular weight
43.747kDa
Background of CD46
CD46 complement regulatory protein also known as CD46 (cluster of differentiation 46) and Membrane Cofactor Protein is a protein which in humans is encoded by the CD46 gene. The protein encoded by this gene is a type I membrane protein and is a regulatory part of the complement system. And the encoded protein has cofactor activity for inactivation of complement components C3b and C4b by serum factor I, which protects the host cell from damage by complement. In addition, the encoded protein can act as a receptor for the Edmonston strain of measles virus, human herpesvirus-6, and type IV pili of pathogenic Neisseria. Finally, the protein encoded by this gene may be involved in the fusion of the spermatozoa with the oocyte during fertilization. Mutations at this locus have been associated with susceptibility to hemolytic uremic syndrome. Alternatively spliced transcript variants encoding different isoforms have been described.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00377-Dyl488 is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (Fixed), 1-3μg/1x106 cells, human
Validation Images & Assay Conditions
Click image to see more details
Boster Kit Box
Protein Target Info & Infographic
Gene/Protein Information For CD46 (Source: Uniprot.org, NCBI)
Gene Name
CD46
Full Name
Membrane cofactor protein
Weight
43.747kDa
Alternative Names
AHUS2 antibody; Antigen defined by monoclonal antibody; TRA 2 10 antibody; Antigen identified by monoclonal antibody; TRA 2 10 antibody; CD46 antibody; CD46 antigen antibody; CD46 antigen complement regulatory protein antibody; CD46 molecule antibody; CD46 molecule complement regulatory protein antibody; Complement membrane cofactor protein antibody; MCP antibody; MCP_HUMAN antibody; Measles virus receptor antibody; membrane cofactor protein (CD46, trophoblast-lymphocyte cross-reactive antigen) antibody; Membrane cofactor protein antibody; MGC26544 antibody;MIC10 antibody; TLX antibody; TRA2.10 antibody; Trophoblast leucocyte common antigen antibody; Trophoblast leukocyte common antigen antibody; Trophoblast lymphocyte cross reactive antigen antibody CD46 AHUS2, MCP, MIC10, TLX, TRA2.10 CD46 molecule membrane cofactor protein|CD46 , complement regulatory protein|CD46 molecule, complement regulatory protein| by TRA-2-10|complement membrane cofactor protein|measles virus receptor|membrane cofactor protein (CD46, trophoblast-lymphocyte cross-reactive )|trophoblast leucocyte common |trophoblast leukocyte common |trophoblast-lymphocyte cross-reactive
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD46, check out the CD46 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD46: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human CD46 DyLight® 488 conjugated Antibody(monoclonal, 9E9) (M00377-Dyl488)
Hello CJ!
No publications found for M00377-Dyl488
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Human CD46 DyLight® 488 conjugated Antibody(monoclonal, 9E9)?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Human CD46 DyLight® 488 conjugated Antibody(monoclonal, 9E9)
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
3 Customer Q&As for Anti-Human CD46 DyLight® 488 conjugated Antibody(monoclonal, 9E9)
Question
We are currently using anti-Human CD46 DyLight® 488 conjugated antibody(monoclonal, 9E9) M00377-Dyl488 for human tissue, and we are content with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2017-09-27
Answer
The anti-Human CD46 DyLight® 488 conjugated antibody(monoclonal, 9E9) (M00377-Dyl488) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-09-27
Question
We have been able to see staining in human sperm. Any tips? Is anti-Human CD46 DyLight® 488 conjugated antibody(monoclonal, 9E9) supposed to stain sperm positively?
Verified Customer
Verified customer
Asked: 2017-06-07
Answer
According to literature sperm does express CD46. According to Uniprot.org, CD46 is expressed in palpebral conjunctiva, testis, teratocarcinoma, fetal kidney, liver salivary gland, liver, sperm, among other tissues. Regarding which tissues have CD46 expression, here are a few articles citing expression in various tissues:
Fetal kidney, Liver, and Salivary gland, Pubmed ID: 17974005
Liver, Pubmed ID: 16710414, 24275569
Sperm, Pubmed ID: 1479546
Teratocarcinoma, Pubmed ID: 14702039
Testis, Pubmed ID: 8418811, 9659228, 15489334
Boster Scientific Support
Answered: 2017-06-07
Question
My team were content with the WB result of your anti-Human CD46 DyLight® 488 conjugated antibody(monoclonal, 9E9). However we have seen positive staining in teratocarcinoma secretory vesicle, using this antibody. Is that expected? Could you tell me where is CD46 supposed to be expressed?
T. Singh
Verified customer
Asked: 2013-03-04
Answer
Based on literature, teratocarcinoma does express CD46. Generally CD46 expresses in cytoplasmic vesicle, secretory vesicle,. Regarding which tissues have CD46 expression, here are a few articles citing expression in various tissues:
Fetal kidney, Liver, and Salivary gland, Pubmed ID: 17974005
Liver, Pubmed ID: 16710414, 24275569
Sperm, Pubmed ID: 1479546
Teratocarcinoma, Pubmed ID: 14702039
Testis, Pubmed ID: 8418811, 9659228, 15489334
Boster Scientific Support
Answered: 2013-03-04
