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Product Info Summary
| SKU: | M00377-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-CD46 Antibody Picoband® (monoclonal, 9E9)
SKU/Catalog Number
M00377-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD46 Antibody Picoband® (monoclonal, 9E9) catalog # M00377-2. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD46 Antibody Picoband® (monoclonal, 9E9) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00377-2)
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Monoclonal
Clone Number
9E9
Isotype
Mouse IgG1
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human CD46.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M00377-2 is reactive to CD46 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Observed Molecular Weight
50-70 kDa
Calculated molecular weight
43.747kDa
Background of CD46
CD46 complement regulatory protein also known as CD46 (cluster of differentiation 46) and Membrane Cofactor Protein is a protein which in humans is encoded by the CD46 gene. The protein encoded by this gene is a type I membrane protein and is a regulatory part of the complement system. And the encoded protein has cofactor activity for inactivation of complement components C3b and C4b by serum factor I, which protects the host cell from damage by complement. In addition, the encoded protein can act as a receptor for the Edmonston strain of measles virus, human herpesvirus-6, and type IV pili of pathogenic Neisseria. Finally, the protein encoded by this gene may be involved in the fusion of the spermatozoa with the oocyte during fertilization. Mutations at this locus have been associated with susceptibility to hemolytic uremic syndrome. Alternatively spliced transcript variants encoding different isoforms have been described.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00377-2 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: human HepG2 tissue,
IHC: human rectum cancer tissue, human liver cancer tissue
FCM: PBMC cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CD46 using anti ZO-1 antibody (M00377-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HepG2 tissue lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CD46 antigen affinity purified polyclonal antibody (Catalog # M00377-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for CD46 at approximately 50-70KD. The expected band size for CD46 is at 44KD.
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Figure 2. IHC analysis of CD46 using anti-CD46 antibody (M00377-2).
DCK was detected in paraffin-embedded section of human rectum cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CD46 Antibody (M00377-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
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Figure 3. IHC analysis of CD46 using anti-CD46 antibody (M00377-2).
DCK was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CD46 Antibody (M00377-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
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Figure 4. Flow Cytometry analysis of PBMC cells using anti-CD46 antibody (M00377-2).
Overlay histogram showing PBMC cells stained with M00377-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-CD46 Antibody (M00377-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For CD46 (Source: Uniprot.org, NCBI)
Gene Name
CD46
Full Name
Membrane cofactor protein
Weight
43.747kDa
Alternative Names
AHUS2 antibody; Antigen defined by monoclonal antibody; TRA 2 10 antibody; Antigen identified by monoclonal antibody; TRA 2 10 antibody; CD46 antibody; CD46 antigen antibody; CD46 antigen complement regulatory protein antibody; CD46 molecule antibody; CD46 molecule complement regulatory protein antibody; Complement membrane cofactor protein antibody; MCP antibody; MCP_HUMAN antibody; Measles virus receptor antibody; membrane cofactor protein (CD46, trophoblast-lymphocyte cross-reactive antigen) antibody; Membrane cofactor protein antibody; MGC26544 antibody;MIC10 antibody; TLX antibody; TRA2.10 antibody; Trophoblast leucocyte common antigen antibody; Trophoblast leukocyte common antigen antibody; Trophoblast lymphocyte cross reactive antigen antibody CD46 AHUS2, MCP, MIC10, TLX, TRA2.10 CD46 molecule membrane cofactor protein|CD46 , complement regulatory protein|CD46 molecule, complement regulatory protein| by TRA-2-10|complement membrane cofactor protein|measles virus receptor|membrane cofactor protein (CD46, trophoblast-lymphocyte cross-reactive )|trophoblast leucocyte common |trophoblast leukocyte common |trophoblast-lymphocyte cross-reactive
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD46, check out the CD46 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD46: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD46 Antibody Picoband® (monoclonal, 9E9) (M00377-2)
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Customer Q&As
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5 Customer Q&As for Anti-CD46 Antibody Picoband® (monoclonal, 9E9)
Question
We are currently using anti-CD46 antibody (monoclonal, 9E9) M00377-2 for human tissue, and we are happy with the IHC-P results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-03-01
Answer
The anti-CD46 antibody (monoclonal, 9E9) (M00377-2) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-03-01
Question
Our lab were content with the WB result of your anti-CD46 antibody (monoclonal, 9E9). However we have observed positive staining in liver cytoplasmic vesicle using this antibody. Is that expected? Could you tell me where is CD46 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-05-24
Answer
According to literature, liver does express CD46. Generally CD46 expresses in cytoplasmic vesicle, secretory vesicle,. Regarding which tissues have CD46 expression, here are a few articles citing expression in various tissues:
Fetal kidney, Liver, and Salivary gland, Pubmed ID: 17974005
Liver, Pubmed ID: 16710414, 24275569
Sperm, Pubmed ID: 1479546
Teratocarcinoma, Pubmed ID: 14702039
Testis, Pubmed ID: 8418811, 9659228, 15489334
Boster Scientific Support
Answered: 2018-05-24
Question
We have observed staining in human liver salivary gland. What should we do? Is anti-CD46 antibody (monoclonal, 9E9) supposed to stain liver salivary gland positively?
Verified Customer
Verified customer
Asked: 2017-12-25
Answer
Based on literature liver salivary gland does express CD46. Based on Uniprot.org, CD46 is expressed in palpebral conjunctiva, testis, teratocarcinoma, fetal kidney, liver salivary gland, liver, sperm, among other tissues. Regarding which tissues have CD46 expression, here are a few articles citing expression in various tissues:
Fetal kidney, Liver, and Salivary gland, Pubmed ID: 17974005
Liver, Pubmed ID: 16710414, 24275569
Sperm, Pubmed ID: 1479546
Teratocarcinoma, Pubmed ID: 14702039
Testis, Pubmed ID: 8418811, 9659228, 15489334
Boster Scientific Support
Answered: 2017-12-25
Question
My lab would like using your anti-CD46 antibody (monoclonal, 9E9) for positive regulation of memory t cell differentiation studies. Has this antibody been tested with western blotting on k562 whole cell lysates? We would like to see some validation images before ordering.
B. Thomas
Verified customer
Asked: 2017-09-13
Answer
I appreciate your inquiry. This M00377-2 anti-CD46 antibody (monoclonal, 9E9) is validated on human placenta tissue, hepg2 whole cell lysates, k562 whole cell lysates, mouse liver tissue, rat heart tissue, kidney tissue, u20s cells, siha cells. It is guaranteed to work for Flow Cytometry, IHC-P, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-09-13
Question
Our lab used your anti-CD46 antibody (monoclonal, 9E9) for WB on testis a few years ago. I am using human, and We are going to use the antibody for Flow Cytometry next. My lab would like examining testis as well as fetal kidney in our next experiment. Could give a recommendation on which antibody would work the best for Flow Cytometry?
L. Li
Verified customer
Asked: 2014-07-31
Answer
I took a look at the website and datasheets of our anti-CD46 antibody (monoclonal, 9E9) and it seems that M00377-2 has been validated on human in both WB and Flow Cytometry. Thus M00377-2 should work for your application. Our Boster satisfaction guarantee will cover this product for Flow Cytometry in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for Flow Cytometry detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2014-07-31
