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Product Info Summary
| SKU: | M00377-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CD46 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00377-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CD46 Rabbit Monoclonal Antibody catalog # M00377-1. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD46 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00377-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AEI-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CD46
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00377-1 is reactive to CD46 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
125 kDa
Calculated molecular weight
43747 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00377-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
IHC: human colon tissue
Validation Images & Assay Conditions
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Western blot analysis of CD46 in K562 cell lysate.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human colon, using CD46 Antibody.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For CD46 (Source: Uniprot.org, NCBI)
Gene Name
CD46
Full Name
Membrane cofactor protein
Weight
43747 MW
Alternative Names
Membrane cofactor protein;TLX;Trophoblast leukocyte common antigen;CD46;CD46;MCP, MIC10; CD46 AHUS2, MCP, MIC10, TLX, TRA2.10 CD46 molecule membrane cofactor protein|CD46 , complement regulatory protein|CD46 molecule, complement regulatory protein| by TRA-2-10|complement membrane cofactor protein|measles virus receptor|membrane cofactor protein (CD46, trophoblast-lymphocyte cross-reactive )|trophoblast leucocyte common |trophoblast leukocyte common |trophoblast-lymphocyte cross-reactive
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD46, check out the CD46 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD46: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD46 Rabbit Monoclonal Antibody (M00377-1)
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No publications found for M00377-1
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-CD46 Rabbit Monoclonal Antibody
Question
Is this M00377-1 anti-CD46 Rabbit Monoclonal antibody reactive to the isotypes of CD46?
G. Krishna
Verified customer
Asked: 2020-02-11
Answer
The immunogen of M00377-1 anti-CD46 Rabbit Monoclonal antibody is A synthesized peptide derived from human CD46. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-02-11
Question
See below the WB image, lot number and protocol we used for fetal kidney using anti-CD46 Rabbit Monoclonal antibody M00377-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-01-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-27
Question
We are currently using anti-CD46 Rabbit Monoclonal antibody M00377-1 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
The anti-CD46 Rabbit Monoclonal antibody (M00377-1) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-16
Question
We have been able to see staining in human palpebral conjunctiva. Are there any suggestions? Is anti-CD46 Rabbit Monoclonal antibody supposed to stain palpebral conjunctiva positively?
N. Krishna
Verified customer
Asked: 2019-12-03
Answer
From what I have seen in literature palpebral conjunctiva does express CD46. From what I have seen in Uniprot.org, CD46 is expressed in palpebral conjunctiva, testis, teratocarcinoma, fetal kidney, liver salivary gland, liver, sperm, among other tissues. Regarding which tissues have CD46 expression, here are a few articles citing expression in various tissues:
Fetal kidney, Liver, and Salivary gland, Pubmed ID: 17974005
Liver, Pubmed ID: 16710414, 24275569
Sperm, Pubmed ID: 1479546
Teratocarcinoma, Pubmed ID: 14702039
Testis, Pubmed ID: 8418811, 9659228, 15489334
Boster Scientific Support
Answered: 2019-12-03
Question
I see that the anti-CD46 Rabbit Monoclonal antibody M00377-1 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-11
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-11
Question
Does M00377-1 anti-CD46 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-02
Answer
As indicated on the product datasheet, M00377-1 anti-CD46 Rabbit Monoclonal antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-02
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for fetal kidney using anti-CD46 Rabbit Monoclonal antibody M00377-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-08
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-08
Question
My question regards to test anti-CD46 Rabbit Monoclonal antibody M00377-1 on human fetal kidney for research purposes, then I may be interested in using anti-CD46 Rabbit Monoclonal antibody M00377-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Williams
Verified customer
Asked: 2019-06-25
Answer
The products we sell, including anti-CD46 Rabbit Monoclonal antibody M00377-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-25
Question
Do you have a BSA free version of anti-CD46 Rabbit Monoclonal antibody M00377-1 available?
Verified Customer
Verified customer
Asked: 2019-06-12
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-CD46 Rabbit Monoclonal antibody M00377-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-12
Question
Can you help my question with product M00377-1, anti-CD46 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-14
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00377-1 anti-CD46 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-14
Question
My colleagues were content with the WB result of your anti-CD46 Rabbit Monoclonal antibody. However we have seen positive staining in teratocarcinoma secretory vesicle, using this antibody. Is that expected? Could you tell me where is CD46 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-05-14
Answer
From literature, teratocarcinoma does express CD46. Generally CD46 expresses in cytoplasmic vesicle, secretory vesicle,. Regarding which tissues have CD46 expression, here are a few articles citing expression in various tissues:
Fetal kidney, Liver, and Salivary gland, Pubmed ID: 17974005
Liver, Pubmed ID: 16710414, 24275569
Sperm, Pubmed ID: 1479546
Teratocarcinoma, Pubmed ID: 14702039
Testis, Pubmed ID: 8418811, 9659228, 15489334
Boster Scientific Support
Answered: 2019-05-14
Question
I was wanting to use your anti-CD46 Rabbit Monoclonal antibody for IHC for human fetal kidney on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human fetal kidney identification?
Verified Customer
Verified customer
Asked: 2017-08-15
Answer
You can see on the product datasheet, M00377-1 anti-CD46 Rabbit Monoclonal antibody has been validated for IF, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human fetal kidney in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-08-15
Question
We purchased anti-CD46 Rabbit Monoclonal antibody for IF on liver in a previous project. I am using human, and We are going to use the antibody for IHC next. I was wanting to use examining liver as well as testis in our next experiment. Do you have any suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2017-07-13
Answer
I looked at the website and datasheets of our anti-CD46 Rabbit Monoclonal antibody and it appears that M00377-1 has been tested on human in both IF and IHC. Thus M00377-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-07-13
Question
I am interested in using your anti-CD46 Rabbit Monoclonal antibody for positive regulation of t cell proliferation studies. Has this antibody been tested with western blotting on k562 cell lysate? We would like to see some validation images before ordering.
A. Evans
Verified customer
Asked: 2016-07-15
Answer
Thank you for your inquiry. This M00377-1 anti-CD46 Rabbit Monoclonal antibody is validated on k562 cell lysate. It is guaranteed to work for IF, IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2016-07-15
Question
Is a blocking peptide available for product anti-CD46 Rabbit Monoclonal antibody (M00377-1)?
E. Gonzalez
Verified customer
Asked: 2016-07-07
Answer
We do provide the blocking peptide for product anti-CD46 Rabbit Monoclonal antibody (M00377-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-07-07
Question
Would anti-CD46 Rabbit Monoclonal antibody M00377-1 work for IHC with fetal kidney?
F. Parker
Verified customer
Asked: 2015-07-27
Answer
According to the expression profile of fetal kidney, CD46 is highly expressed in fetal kidney. So, it is likely that anti-CD46 Rabbit Monoclonal antibody M00377-1 will work for IHC with fetal kidney.
Boster Scientific Support
Answered: 2015-07-27
