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Product Info Summary
| SKU: | EK1888 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse CPE/Carboxypeptidase E ELISA Kit PicoKine®
View all Carboxypeptidase E/CPE ELISA kits
SKU/Catalog Number
EK1888
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse CPE ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Cpe in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse CPE/Carboxypeptidase E ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1888)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: L43-F476
Sensitivity
<50 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1888 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1888 is reactive to Cpe in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK1888 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Carboxypeptidase E/CPE
CPE encodes carboxypeptidase E, a prohormone-processing exopeptidase found in secretory granules of endocrine and neuroendocrine cells. It is mapped to 8 B3.1; 8 32.3 cM. The encoded preproprotein undergoes proteolytic processing to generate a mature, functional enzyme that cleaves the C-terminal basic residues of protein substrates. A missense mutation in this gene is responsible for the obesity phenotype in a mouse model known as the "fat mouse." Mice lacking the functional product of this gene exhibit impaired processing of multiple peptide hormones such as proinsulin, prodynorphin, proneurotensin, promelanin-concentrating hormone and pro-opiomelanocortin.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1888-CAP | Anti-Mouse Cpe Pre-coated 96-well strip microplate | 1 |
| EK1888-ST | Mouse Cpe Standard | 2 vials, 10 ng/tube |
| EK1888-DA | Mouse Cpe Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.013 | 0.068 | 0.119 | 0.202 | 0.409 | 0.787 | 1.459 | 2.189 |
Data Example Images
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Mouse CPE PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Carboxypeptidase E/CPE, Dilution ratio of 1:1, concentration in serum and plasma is 400-1000 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse CPE/Carboxypeptidase E ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 91 | 252 | 873 | 95 | 258 | 907 |
| Standard deviation | 5.92 | 12.85 | 55.00 | 5.23 | 17.54 | 68.03 |
| CV (%) | 6.5% | 5.1% | 6.3% | 5.5% | 6.8% | 7.5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Carboxypeptidase E/CPE in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 91 | 94 | 82 | 89 | 89 | 4.41 | 4.9% |
| Sample 2 | 252 | 268 | 294 | 252 | 266 | 17.16 | 6.4% |
| Sample 3 | 873 | 901 | 1007 | 933 | 928 | 50.04 | 5.3% |
Protein Target Info & Infographic
Gene/Protein Information For Cpe (Source: Uniprot.Org, NCBI)
Gene Name
Cpe
Full Name
Carboxypeptidase E
Weight
53.151kDa
Superfamily
peptidase M14 family
Alternative Names
Carboxypeptidase E; CPE; Carboxypeptidase H; CPH; Enkephalin convertase; Prohormone-processing carboxypeptidase CPE CPH carboxypeptidase E carboxypeptidase E|carbocypeptidase E|carboxypeptidase H|cobalt-stimulated chromaffin granule carboxypeptidase|enkephalin convertase|epididymis secretory sperm binding protein|insulin granule-associated carboxypeptidase|prohormone-processing carboxypeptidase
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Cpe, check out the Cpe Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Cpe: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse CPE/Carboxypeptidase E ELISA Kit PicoKine® (EK1888)
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Customer Q&As
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8 Customer Q&As for Mouse CPE/Carboxypeptidase E ELISA Kit PicoKine®
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-07-24
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-07-24
Question
Q: if the enzyme conjugated Carboxypeptidase E antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Carboxypeptidase E antigen?
Verified Customer
Verified customer
Asked: 2020-03-14
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-03-14
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2019-04-12
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-04-12
Question
Q: What is the optimal O.D. value for Carboxypeptidase E ELISA kit? I performed your Carboxypeptidase E ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Carboxypeptidase E even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2018-08-20
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Carboxypeptidase E ELISA kit to have sensitivity of 50pg/ml, that means the minimum amount of Carboxypeptidase E that can be declared/interpreted as positive by the above standard is 50pg/ml.
Boster Scientific Support
Answered: 2018-08-20
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2018-07-20
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-07-20
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring Carboxypeptidase E using the EK1888 Mouse Carboxypeptidase E Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-03-08
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2018-03-08
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
M. Singh
Verified customer
Asked: 2017-08-15
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2017-08-15
Question
Q: how to thaw whole blood sample for Carboxypeptidase E ELISA after freezing?
Verified Customer
Verified customer
Asked: 2014-05-05
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Carboxypeptidase E for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2014-05-05
