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Product Info Summary
| SKU: | A07570-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-RTCD1/RTCA Antibody Picoband®
SKU/Catalog Number
A07570-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-RTCD1/RTCA Antibody Picoband® catalog # A07570-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RTCD1/RTCA Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07570-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human RTCD1/RTCA recombinant protein (Position: Q18-L366).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A07570-1 is reactive to RTCA in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
39 kDa
Calculated molecular weight
157578 MW
Background of RTCD1
This gene encodes a member of the RNA 3'-phosphate cyclase family. The encoded protein plays a role in RNA metabolism by catalyzing the ATP-dependent conversion of the 3'-phosphate of RNA substrates to a 2',3'-cyclic phosphodiester. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07570-1 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human U-87MG whole cell, human THP-1 whole cell, human A431 whole cell
ICC/IF: PC-3 cell
FCM: CACO-2 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of RTCD1/RTCA using anti-RTCD1/RTCA antibody (A07570-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human U-87MG whole cell lysates,
Lane 2: human THP-1 whole cell lysates,
Lane 3: human A431 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RTCD1/RTCA antigen affinity purified polyclonal antibody (Catalog # A07570-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RTCD1/RTCA at approximately 39KD. The expected band size for RTCD1/RTCA is at 39KD.
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Figure 2. IF analysis of RTCD1/RTCA using anti-RTCD1/RTCA antibody (A07570-1).
RTCD1/RTCA was detected in immunocytochemical section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-RTCD1/RTCA Antibody (A07570-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. Flow Cytometry analysis of CACO-2 cells using anti-RTCD1/RTCA antibody (A07570-1).
Overlay histogram showing CACO-2 cells stained with A07570-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RTCD1/RTCA Antibody (A07570-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For RTCA (Source: Uniprot.org, NCBI)
Gene Name
RTCA
Full Name
RNA 3'-terminal phosphate cyclase
Weight
157578 MW
Superfamily
RNA 3'-terminal cyclase family
Alternative Names
C-C motif chemokine 16; Chemokine CC-4; HCC-4; Chemokine LEC; IL-10-inducible chemokine; LCC-1; Liver-expressed chemokine; Lymphocyte and monocyte chemoattractant; LMC; Monotactin-1; MTN-1; NCC-4; Small-inducible cytokine A16; CCL16; ILINCK; NCC4; SCYA16 RTCA RPC, RTC1, RTCD1 RNA 3-terminal phosphate cyclase RNA 3-terminal phosphate cyclase|RNA terminal phosphate cyclase domain 1|RNA terminal phosphate cyclase domain-containing protein 1|RTC domain-containing protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RTCA, check out the RTCA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RTCA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RTCD1/RTCA Antibody Picoband® (A07570-1)
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