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Product Info Summary
| SKU: | M00377 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, Functional Assay |
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Product info
Product Name
Anti-CD46 (Membrane Cofactor Protein) Monoclonal Antibody
SKU/Catalog Number
M00377
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-CD46 (Membrane Cofactor Protein) Monoclonal Antibody (Catalog # M00377). Tested in Functional Assay, Flow Cytometry, IF applications. This antibody reacts with Human.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-CD46 (Membrane Cofactor Protein) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00377)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: 122.2
Isotype
IgG1, kappa
Immunogen
Recombinant human CD46 protein
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00377 is reactive to CD46 in Human
Applications
M00377 is guaranteed for Flow Cytometry, IF, Functional Assay Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
43747 MW
Background of CD46
CD46 acts as a cofactor for complement factor I, a serine protease, which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. It may be involved in the fusion of the spermatozoa with the oocyte during fertilization. CD46 acts as a co-stimulatory factor for T-cells, which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity. A number of viral and bacterial pathogens seem to exploit this property and directly induce an immunosuppressive phenotype in T-cells by binding to CD46.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Reconstitute with 1ml water (15 minutes at room temperature).
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Functional Studies (Order Ab without Azide)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
Click image to see more details
FCM staining of human PBMCs using Anti-CD46 Mouse Monoclonal Antibody (122.2).
Protein Target Info & Infographic
Gene/Protein Information For CD46 (Source: Uniprot.org, NCBI)
Gene Name
CD46
Full Name
Membrane cofactor protein
Weight
43747 MW
Alternative Names
AHUS2; CD46 antigen; CD46 molecule, complement regulatory protein; CD46; MCP; membrane cofactor protein (CD46, trophoblast-lymphocyte cross-reactive antigen); MIC10; TLXcomplement regulatory protein; trophoblast leucocyte common antigen; Trophoblast leukocyte common antigen; trophoblast-lymphocyte cross-reactive antigen CD46 AHUS2, MCP, MIC10, TLX, TRA2.10 CD46 molecule membrane cofactor protein|CD46 antigen, complement regulatory protein|CD46 molecule, complement regulatory protein|antigen identified by monoclonal antibody TRA-2-10|complement membrane cofactor protein|measles virus receptor|membrane cofactor protein (CD46, trophoblast-lymphocyte cross-reactive antigen)|trophoblast leucocyte common antigen|trophoblast leukocyte common antigen|trophoblast-lymphocyte cross-reactive antigen
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD46, check out the CD46 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD46: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD46 (Membrane Cofactor Protein) Monoclonal Antibody (M00377)
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No publications found for M00377
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Customer Q&As
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14 Customer Q&As for Anti-CD46 (Membrane Cofactor Protein) Monoclonal Antibody
Question
Do you have a BSA free version of anti-CD46 (Membrane Cofactor Protein) Monoclonal antibody M00377 available?
Verified Customer
Verified customer
Asked: 2020-05-06
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-CD46 (Membrane Cofactor Protein) Monoclonal antibody M00377 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-05-06
Question
We are currently using anti-CD46 (Membrane Cofactor Protein) Monoclonal antibody M00377 for human tissue, and we are happy with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-21
Answer
The anti-CD46 (Membrane Cofactor Protein) Monoclonal antibody (M00377) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-21
Question
Would M00377 anti-CD46 (Membrane Cofactor Protein) Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-04-07
Answer
You can see on the product datasheet, M00377 anti-CD46 (Membrane Cofactor Protein) Monoclonal antibody as been validated on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-07
Question
I am interested in to test anti-CD46 (Membrane Cofactor Protein) Monoclonal antibody M00377 on human liver for research purposes, then I may be interested in using anti-CD46 (Membrane Cofactor Protein) Monoclonal antibody M00377 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
The products we sell, including anti-CD46 (Membrane Cofactor Protein) Monoclonal antibody M00377, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-13
Question
I have a question about product M00377, anti-CD46 (Membrane Cofactor Protein) Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00377 anti-CD46 (Membrane Cofactor Protein) Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-03
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-CD46 (Membrane Cofactor Protein) Monoclonal antibody M00377. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-26
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-26
Question
Is a blocking peptide available for product anti-CD46 (Membrane Cofactor Protein) Monoclonal antibody (M00377)?
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
We do provide the blocking peptide for product anti-CD46 (Membrane Cofactor Protein) Monoclonal antibody (M00377). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-09-19
Question
I see that the anti-CD46 (Membrane Cofactor Protein) Monoclonal antibody M00377 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-12
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-12
Question
Our lab were content with the WB result of your anti-CD46 (Membrane Cofactor Protein) Monoclonal antibody. However we have been able to see positive staining in palpebral conjunctiva cytoplasmic vesicle using this antibody. Is that expected? Could you tell me where is CD46 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-06-19
Answer
According to literature, palpebral conjunctiva does express CD46. Generally CD46 expresses in cytoplasmic vesicle, secretory vesicle,. Regarding which tissues have CD46 expression, here are a few articles citing expression in various tissues:
Fetal kidney, Liver, and Salivary gland, Pubmed ID: 17974005
Liver, Pubmed ID: 16710414, 24275569
Sperm, Pubmed ID: 1479546
Teratocarcinoma, Pubmed ID: 14702039
Testis, Pubmed ID: 8418811, 9659228, 15489334
Boster Scientific Support
Answered: 2019-06-19
Question
Is this M00377 anti-CD46 (Membrane Cofactor Protein) Monoclonal antibody reactive to the isotypes of CD46?
Verified Customer
Verified customer
Asked: 2019-06-05
Answer
The immunogen of M00377 anti-CD46 (Membrane Cofactor Protein) Monoclonal antibody is Recombinant human CD46 protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-05
Question
We have observed staining in human teratocarcinoma. Are there any suggestions? Is anti-CD46 (Membrane Cofactor Protein) Monoclonal antibody supposed to stain teratocarcinoma positively?
Verified Customer
Verified customer
Asked: 2019-05-10
Answer
From what I have seen in literature teratocarcinoma does express CD46. From what I have seen in Uniprot.org, CD46 is expressed in palpebral conjunctiva, testis, teratocarcinoma, fetal kidney, liver salivary gland, liver, sperm, among other tissues. Regarding which tissues have CD46 expression, here are a few articles citing expression in various tissues:
Fetal kidney, Liver, and Salivary gland, Pubmed ID: 17974005
Liver, Pubmed ID: 16710414, 24275569
Sperm, Pubmed ID: 1479546
Teratocarcinoma, Pubmed ID: 14702039
Testis, Pubmed ID: 8418811, 9659228, 15489334
Boster Scientific Support
Answered: 2019-05-10
Question
I was wanting to use your anti-CD46 (Membrane Cofactor Protein) Monoclonal antibody for Flow Cytometry for human liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human liver identification?
Verified Customer
Verified customer
Asked: 2018-03-26
Answer
You can see on the product datasheet, M00377 anti-CD46 (Membrane Cofactor Protein) Monoclonal antibody has been validated for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-03-26
Question
Would anti-CD46 (Membrane Cofactor Protein) Monoclonal antibody M00377 work for Flow Cytometry with liver?
O. Parker
Verified customer
Asked: 2017-03-29
Answer
According to the expression profile of liver, CD46 is highly expressed in liver. So, it is likely that anti-CD46 (Membrane Cofactor Protein) Monoclonal antibody M00377 will work for Flow Cytometry with liver.
Boster Scientific Support
Answered: 2017-03-29
Question
Here is the WB image, lot number and protocol we used for liver using anti-CD46 (Membrane Cofactor Protein) Monoclonal antibody M00377. Please let me know if you require anything else.
G. Miller
Verified customer
Asked: 2016-06-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-06-20
