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Product Info Summary
| SKU: | EK1413 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human CD5L/CT-2 ELISA Kit PicoKine®
SKU/Catalog Number
EK1413
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CD5L / CT 2 ELISA Kit PicoKine® (96 Tests). Quantitate Human CD5L in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CD5L/CT-2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1413)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: S20-G347
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1413 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1413 is reactive to CD5L in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1413 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CD5L
CD5 antigen-like, also known as Sp alpha and AIM, is a protein that in humans is encoded by the CD5L gene. It is mapped to 1q21-q23 by fluorescence in situ hybridization. It is found that Aim expression is induced in mouse macrophages in response to loading with highly oxidized low density lipoprotein (oxLDL), and that Aim is expressed in foam cells within atherosclerotic lesions. Both the expression of Aim in lesions and its induction by oxLDL require Lxr /Rxr heterodimers. Aim-null macrophages are highly susceptible to oxLDL-induced apoptosis in vitro and undergo accelerated apoptosis in atherosclerotic lesions in vivo. Double knockout of Aim and Ldlr reduce atherosclerotic lesions. Therefore, it is concluded that AIM expression protects macrophages from apoptosis within atherosclerotic lesions, promoting early lesion development.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1413-CAP | Anti-Human CD5L Pre-coated 96-well strip microplate | 1 |
| EK1413-ST | Human CD5L Standard | 2 vials, 10 ng/tube |
| EK1413-DA | Human CD5L Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.008 | 0.433 | 0.677 | 1.005 | 1.409 | 1.650 | 1.849 | 2.099 |
Data Example Images
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Human CD5L PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CD5L, Dilution ratio of 1:1000, concentration in serum and plasma is 1000-2500 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CD5L/CT-2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 245 | 1172 | 5450 | 224 | 1288 | 5192 |
| Standard deviation | 10.04 | 79.69 | 414.2 | 13.21 | 91.44 | 415.36 |
| CV (%) | 4.1% | 6.8% | 7.6% | 5.9% | 7.1% | 8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CD5L in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 245 | 242 | 250 | 244 | 245 | 2.94 | 1.2% |
| Sample 2 | 1172 | 1254 | 1148 | 1193 | 1191 | 39.3 | 3.2% |
| Sample 3 | 5450 | 5193 | 4994 | 5419 | 5264 | 184.77 | 3.5% |
Protein Target Info & Infographic
Gene/Protein Information For CD5L (Source: Uniprot.Org, NCBI)
Gene Name
CD5L
Full Name
CD5 antigen-like
Weight
38088 MW
Alternative Names
CD5 antigen-like;Apoptosis inhibitor expressed by macrophages ;hAIM ;CT-2 ;IgM-associated peptide ;SP-alpha ;CD5L;API6;UNQ203/PRO229 ; CD5L AIM, API6, CT-2, PRO229, SP-ALPHA, Spalpha, hAIM CD5 molecule like CD5 -like|CD5 -like (scavenger receptor cysteine rich family)|apoptosis inhibitor 6|apoptosis inhibitor expressed by macrophages|apoptosis inhibitor of macrophage|igM-associated peptide
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CD5L, check out the CD5L Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CD5L: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CD5L/CT-2 ELISA Kit PicoKine® (EK1413)
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No publications found for EK1413
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Human CD5L/CT-2 ELISA Kit PicoKine®
Question
Q: how can I thaw whole blood sample for CD5L ELISA after freezing?
Verified Customer
Verified customer
Asked: 2020-12-05
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CD5L for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2020-12-05
Question
Q: What is the optimal O.D. value for CD5L ELISA kit? I performed your CD5L ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain CD5L even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2020-08-17
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CD5L ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of CD5L that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2020-08-17
Question
Q: how to proceed with the analysis of ELISA data? I have performed CD5L level in plasma.
Verified Customer
Verified customer
Asked: 2020-05-29
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-05-29
Question
Q: if the enzyme conjugated CD5L antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CD5L antigen?
Verified Customer
Verified customer
Asked: 2020-04-15
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-04-15
Question
Does EK1413, measure total CD5L in the serum/plasma or the free form only?
Verified Customer
Verified customer
Asked: 2020-02-14
Answer
EK1413 measures the total CD5L in the serum/plasma.
Boster Scientific Support
Answered: 2020-02-14
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
R. Collins
Verified customer
Asked: 2018-04-12
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-04-12
Question
Q: Can CD5L ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
M. Taylor
Verified customer
Asked: 2016-06-30
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2016-06-30
