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Product Info Summary
| SKU: | A00478-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-FGF23 Antibody Picoband®
SKU/Catalog Number
A00478-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-FGF23 Antibody Picoband® catalog # A00478-3. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FGF23 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00478-3)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived rat FGF23 recombinant protein (Position: Y25-V251).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00478-3 is reactive to Fgf23 in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
35 kDa
Calculated molecular weight
24145 MW
Background of FGF-23
Fibroblast growth factor 23orFGF23is aproteinthat in humans is encoded by theFGF23gene. This gene encodes a member of the fibroblast growth factor family of proteins, which possess broad mitogenic and cell survival activities and are involved in a variety of biological processes. The product of this gene regulates phosphate homeostasis and transport in the kidney. The full-length, functional protein may be deactivated via cleavage into N-terminal and C-terminal chains. Mutation of this cleavage site causes autosomal dominant hypophosphatemic rickets (ADHR). Mutations in this gene are also associated with hyperphosphatemic familial tumoral calcinosis (HFTC).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00478-3 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: rat testis tissue, rat PC-12 whole cell, mouse testis tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of FGF23 using anti-FGF23 antibody (A00478-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat testis tissue lysate,
Lane 2: rat PC-12 whole cell lysate,
Lane 3: mouse testis tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF23 antigen affinity purified polyclonal antibody (Catalog # A00478-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF23 at approximately 35KD. The expected band size for FGF23 is at 28KD.
Protein Target Info & Infographic
Gene/Protein Information For Fgf23 (Source: Uniprot.org, NCBI)
Gene Name
Fgf23
Full Name
Fibroblast growth factor 23
Weight
24145 MW
Superfamily
heparin-binding growth factors family
Alternative Names
Fibroblast growth factor 23; FGF-23; Fgf23 FGF23 ADHR, FGFN, HFTC2, HPDR2, HYPF, PHPTC fibroblast growth factor 23 fibroblast growth factor 23|phosphatonin|tumor-derived hypophosphatemia inducing factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Fgf23, check out the Fgf23 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Fgf23: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FGF23 Antibody Picoband® (A00478-3)
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No publications found for A00478-3
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-FGF23 Antibody Picoband®
Question
Is a blocking peptide available for product anti-FGF23 antibody (A00478-3)?
G. Dhar
Verified customer
Asked: 2020-01-28
Answer
We do provide the blocking peptide for product anti-FGF23 antibody (A00478-3). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-28
Question
Will anti-FGF23 antibody A00478-3 work on monkey WB with right atrium auricular region?
Verified Customer
Verified customer
Asked: 2020-01-13
Answer
Our lab technicians have not tested anti-FGF23 antibody A00478-3 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-FGF23 antibody A00478-3 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey right atrium auricular region in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-13
Question
I was wanting to use to test anti-FGF23 antibody A00478-3 on rat right atrium auricular region for research purposes, then I may be interested in using anti-FGF23 antibody A00478-3 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-06
Answer
The products we sell, including anti-FGF23 antibody A00478-3, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-06
Question
I was wanting to use your anti-FGF23 antibody for ELISA for rat right atrium auricular region on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat right atrium auricular region identification?
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
You can see on the product datasheet, A00478-3 anti-FGF23 antibody has been validated for ELISA, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat right atrium auricular region in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-08
Question
Please see the WB image, lot number and protocol we used for right atrium auricular region using anti-FGF23 antibody A00478-3. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-09-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-24
Question
My question regarding product A00478-3, anti-FGF23 antibody . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-08
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00478-3 anti-FGF23 antibody , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-08
Question
Is this A00478-3 anti-FGF23 antibody reactive to the isotypes of FGF23?
Verified Customer
Verified customer
Asked: 2019-06-03
Answer
The immunogen of A00478-3 anti-FGF23 antibody is E. coli-derived rat FGF23 recombinant protein (Position: Y25-V251). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-03
Question
Will A00478-3 anti-FGF23 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-24
Answer
It shows on the product datasheet, A00478-3 anti-FGF23 antibody as been validated on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-24
Question
Does anti-FGF23 antibody A00478-3 work for ELISA with right atrium auricular region?
Verified Customer
Verified customer
Asked: 2019-01-29
Answer
According to the expression profile of right atrium auricular region, FGF23 is highly expressed in right atrium auricular region. So, it is likely that anti-FGF23 antibody A00478-3 will work for ELISA with right atrium auricular region.
Boster Scientific Support
Answered: 2019-01-29
Question
We would like to know if these three products can be used in IHC on rats: https://www.bosterbio.com/anti-fgf23-picoband-trade-antibody-a00478-3-boster.html#gene-info https://www.bosterbio.com/anti-nr3c2-picoband-trade-antibody-pb9765-boster.html?options=cart https://www.bosterbio.com/anti-nr3c2-antibody-pa1594-boster.html?options=cart
Verified Customer
Verified customer
Asked: 2018-03-13
Answer
A00478-3, PB9765, and PA1594 can not be used for IHC on rats.
Boster Scientific Support
Answered: 2018-03-13
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for right atrium auricular region using anti-FGF23 antibody A00478-3. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-01-29
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-01-29
Question
We are currently using anti-FGF23 antibody A00478-3 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2017-11-01
Answer
The anti-FGF23 antibody (A00478-3) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-11-01
Question
I am interested in using your anti-FGF23 antibody for cell differentiation studies. Has this antibody been tested with western blotting on rat testis tissue? We would like to see some validation images before ordering.
C. Dhar
Verified customer
Asked: 2015-11-30
Answer
Thanks for your inquiry. This A00478-3 anti-FGF23 antibody is validated on rat testis tissue, tissue lysate, mouse testis tissue. It is guaranteed to work for ELISA, WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2015-11-30
Question
I see that the anti-FGF23 antibody A00478-3 works with ELISA, what is the protocol used to produce the result images on the product page?
M. Krishna
Verified customer
Asked: 2015-04-01
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2015-04-01
Question
Do you have a BSA free version of anti-FGF23 antibody A00478-3 available?
A. Brown
Verified customer
Asked: 2014-08-18
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-FGF23 antibody A00478-3 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-08-18
