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Product Info Summary
| SKU: | M00574-Dyl550 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-Human CASR DyLight® 550 conjugated Antibody(monoclonal, 11E9)
View all Calcium-sensing R/CaSR Antibodies
SKU/Catalog Number
M00574-Dyl550
Size
100 μg/vial
Form
Liquid
Description
Boster Bio Anti-Human CASR DyLight® 550 conjugated Antibody (monoclonal, 11E9) catalog # M00574-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Cite This Product
Anti-Human CASR DyLight® 550 conjugated Antibody(monoclonal, 11E9) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00574-Dyl550)
Host
Mouse
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
Clonality
Monoclonal
Clone Number
Clone: 11E9
Isotype
Mouse IgG2b
Immunogen
E. coli-derived human CASR recombinant protein (Position: Q926-S1078). Human CASR shares 80.5% and 78.6% amino acid (aa) sequence identity with mouse and rat CASR, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M00574-Dyl550 is reactive to CASR in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
120.675kDa
Background of Calcium-sensing R/CaSR
The calcium-sensing receptor (CaSR) is a G protein-coupled receptor that is expressed in the parathyroid hormone (PTH)-producing chief cells of the parathyroid gland, and the cells lining the kidney tubule. It senses small changes in circulating calcium concentration and couples this information to intracellular signaling pathways that modify PTH secretion or renal cation handling, thus this protein plays an essential role in maintaining mineral ion homeostasis. Mutations in this gene cause familial hypocalciuric hypercalcemia, familial, isolated hypoparathyroidism, and neonatal severe primary hyperparathyroidism.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00574-Dyl550 is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (Fixed), 1-3μg/1x106 cells
Validation Images & Assay Conditions
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Boster Kit Box
Protein Target Info & Infographic
Gene/Protein Information For CASR (Source: Uniprot.org, NCBI)
Gene Name
CASR
Full Name
Extracellular calcium-sensing receptor
Weight
120.675kDa
Superfamily
G-protein coupled receptor 3 family
Alternative Names
Extracellular calcium-sensing receptor; CaR CASR CAR, EIG8, FHH, FIH, GPRC2A, HHC, HHC1, HYPOC1, NSHPT, PCAR1, hCasR calcium sensing receptor extracellular calcium-sensing receptor|parathyroid Ca(2+)-sensing receptor 1|parathyroid cell calcium-sensing receptor 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CASR, check out the CASR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CASR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human CASR DyLight® 550 conjugated Antibody(monoclonal, 11E9) (M00574-Dyl550)
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No publications found for M00574-Dyl550
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-Human CASR DyLight® 550 conjugated Antibody(monoclonal, 11E9)
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-Human CASR DyLight® 550 conjugated antibody(monoclonal, 11E9) M00574-Dyl550. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-03
Question
Is a blocking peptide available for product anti-Human CASR DyLight® 550 conjugated antibody(monoclonal, 11E9) (M00574-Dyl550)?
Verified Customer
Verified customer
Asked: 2019-12-05
Answer
We do provide the blocking peptide for product anti-Human CASR DyLight® 550 conjugated antibody(monoclonal, 11E9) (M00574-Dyl550). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-12-05
Question
Does anti-Human CASR DyLight® 550 conjugated antibody(monoclonal, 11E9) M00574-Dyl550 work for Flow Cytometry with brain?
Verified Customer
Verified customer
Asked: 2019-07-19
Answer
According to the expression profile of brain, CASR is highly expressed in brain. So, it is likely that anti-Human CASR DyLight® 550 conjugated antibody(monoclonal, 11E9) M00574-Dyl550 will work for Flow Cytometry with brain.
Boster Scientific Support
Answered: 2019-07-19
Question
Does M00574-Dyl550 anti-Human CASR DyLight® 550 conjugated antibody(monoclonal, 11E9) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
O. Mangal
Verified customer
Asked: 2019-03-21
Answer
As indicated on the product datasheet, M00574-Dyl550 anti-Human CASR DyLight® 550 conjugated antibody(monoclonal, 11E9) as been tested on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-03-21
Question
Please see the WB image, lot number and protocol we used for brain using anti-Human CASR DyLight® 550 conjugated antibody(monoclonal, 11E9) M00574-Dyl550. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-03-07
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-03-07
