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Product Info Summary
| SKU: | PB9868 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-FGF23 Antibody Picoband®
SKU/Catalog Number
PB9868
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-FGF23 Antibody Picoband® catalog # PB9868. Tested in WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FGF23 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9868)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse FGF23 recombinant protein (Position: R48-V251). Mouse FGF23 shares 71.1% and 94.6% amino acid (aa) sequence identity with human and rat FGF23, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9868 is reactive to Fgf23 in Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
34 kDa
Calculated molecular weight
27758 MW
Background of FGF-23
Fibroblast growth factor 23 or FGF23 is a protein that in humans is encoded by the FGF23 gene. This gene encodes a member of the fibroblast growth factor family of proteins, which possess broad mitogenic and cell survival activities and are involved in a variety of biological processes. The product of this gene regulates phosphate homeostasis and transport in the kidney. The full-length, functional protein may be deactivated via cleavage into N-terminal and C-terminal chains. Mutation of this cleavage site causes autosomal dominant hypophosphatemic rickets (ADHR). Mutations in this gene are also associated with hyperphosphatemic familial tumoral calcinosis (HFTC).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9868 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse
Positive Control
WB: mouse testis tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of FGF 23 using anti-FGF 23 antibody (PB9868).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF 23 antigen affinity purified polyclonal antibody (Catalog # PB9868) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF 23 at approximately 34 kDa. The expected band size for FGF 23 is at 28 kDa.
Protein Target Info & Infographic
Gene/Protein Information For Fgf23 (Source: Uniprot.org, NCBI)
Gene Name
Fgf23
Full Name
Fibroblast growth factor 23
Weight
27758 MW
Superfamily
heparin-binding growth factors family
Alternative Names
Fibroblast growth factor 23;FGF-23;Fgf23; FGF23 ADHR, FGFN, HFTC2, HPDR2, HYPF, PHPTC fibroblast growth factor 23 fibroblast growth factor 23|phosphatonin|tumor-derived hypophosphatemia inducing factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Fgf23, check out the Fgf23 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Fgf23: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FGF23 Antibody Picoband® (PB9868)
Hello CJ!
PB9868 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Ji KB, Ling L, Zhang Q, Chou JJ, Yang XL, Wang ZH, Yin L, Wu SF, Yu YF. Exp Ther Med. 2018 Jan;15(1):139-144. doi: 10.3892/etm.2017.5408. Epub 2017 Oct 31. MicroRNA-296 mediated corneal neovascularization in an animal model of corneal burns after ...
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Customer Q&As
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1 Customer Q&As for Anti-FGF23 Antibody Picoband®
Question
We are currently using anti-FGF23 antibody PB9868 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says mouse. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2017-08-03
Answer
The anti-FGF23 antibody (PB9868) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-08-03
