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Product Info Summary
| SKU: | EK1362 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse FGF23 ELISA Kit PicoKine®
SKU/Catalog Number
EK1362
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse FGF23 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Fgf23 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse FGF23 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1362)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: Y25-V251
Sensitivity
<10 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1362 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1362 is reactive to Fgf23 in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1362 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of FGF-23
Fibroblast growth factor 23 or FGF23 is a protein that in humans is encoded by the FGF23 gene. It is a member of the fibroblast growth factor (FGF) family which is responsible for phosphate metabolism. The main function of FGF23 seems to be regulation of phosphate concentration in plasma. FGF23 is secreted by Osteocytes in response to elevated Calcitriol. And it acts on the kidneys, where it decreases the expression of NPT2, a sodium-phosphate cotransporter in the proximal tubule. Thus, FGF23 decreases the reabsorption and increases excretion of phosphate. Also, FGF23 may suppress 1-alpha-hydroxylase, reducing its ability to activate vitamin D and subsequently impairing calcium absorption.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1362-CAP | Anti-Mouse Fgf23 Pre-coated 96-well strip microplate | 1 |
| EK1362-ST | Mouse Fgf23 Standard | 2 vials, 10 ng/tube |
| EK1362-DA | Mouse Fgf23 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.029 | 0.069 | 0.125 | 0.223 | 0.328 | 0.622 | 1.054 | 1.863 |
Data Example Images
Click image to see more details
Mouse FGF23 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect FGF-23, Dilution ratio of 1:1, concentration in serum and plasma is 40-150 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse FGF23 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 35 | 216 | 449 | 33 | 213 | 449 |
| Standard deviation | 2.73 | 8.85 | 35.47 | 2.77 | 12.78 | 35.47 |
| CV (%) | 7.8% | 4.1% | 7.9% | 8.4% | 6% | 7.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of FGF-23 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 35 | 29 | 31 | 34 | 32 | 2.38 | 7.4% |
| Sample 2 | 216 | 235 | 249 | 229 | 232 | 11.86 | 5.1% |
| Sample 3 | 449 | 395 | 424 | 400 | 417 | 21.48 | 5.1% |
Protein Target Info & Infographic
Gene/Protein Information For Fgf23 (Source: Uniprot.Org, NCBI)
Gene Name
Fgf23
Full Name
Fibroblast growth factor 23
Weight
27758 MW
Superfamily
heparin-binding growth factors family
Alternative Names
Fibroblast growth factor 23;FGF-23;Fgf23; FGF23 ADHR, FGFN, HFTC2, HPDR2, HYPF, PHPTC fibroblast growth factor 23 fibroblast growth factor 23|phosphatonin|tumor-derived hypophosphatemia inducing factor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Fgf23, check out the Fgf23 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Fgf23: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse FGF23 ELISA Kit PicoKine® (EK1362)
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Customer Reviews
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Customer Q&As
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13 Customer Q&As for Mouse FGF23 ELISA Kit PicoKine®
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-05-09
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-05-09
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2020-01-22
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-01-22
Question
Q: how can I thaw whole blood sample for FGF-23 ELISA after freezing?
K. Martinez
Verified customer
Asked: 2019-12-02
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test FGF-23 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2019-12-02
Question
Q: how to analyze ELISA data? I have performed FGF-23 level in plasma.
M. Carter
Verified customer
Asked: 2019-11-15
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-11-15
Question
We
Verified Customer
Verified customer
Asked: 2018-02-08
Answer
We think the kit EK1362 detects the full length of FGF23, both N-terminal and C-terminal FGF23.
Boster Scientific Support
Answered: 2018-02-08
Question
Q: if the enzyme conjugated FGF-23 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the FGF-23 antigen?
M. Mitchell
Verified customer
Asked: 2017-03-28
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2017-03-28
Question
Q: Can FGF-23 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2016-11-20
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2016-11-20
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
D. Jones
Verified customer
Asked: 2016-08-19
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2016-08-19
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2016-06-29
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2016-06-29
Question
Q: is it okay to use citrate plasma as samples in Mouse FGF-23 Picokine® ELISA Kit (Catalog # EK1362)?
W. Roberts
Verified customer
Asked: 2016-03-03
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of FGF-23 causing disruption of its protein structure. FGF-23 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the FGF-23 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2016-03-03
Question
I would like to confirm if this FGF23 ELISA kit detects intact FGF23 (antibody binds to N-terminal) or the C-terminal FGF23?
Verified Customer
Verified customer
Asked: 2014-06-20
Answer
The kit EK1362 detects the full length of FGF23, both N-terminal and C-terminal FGF23.
Boster Scientific Support
Answered: 2014-06-20
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification FGF-23 using the EK1362 Mouse FGF-23 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2014-05-08
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2014-05-08
Question
Does EK1362 recognize iFGF23 or cFGF23? Keywords: intact, C-terminal, C-terminus, detection
Verified Customer
Verified customer
Asked: 2013-03-18
Answer
EK1362 recognizes iFGF23.
Boster Scientific Support
Answered: 2013-03-18
