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Product Info Summary
| SKU: | EK0759 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human FGF23 ELISA Kit PicoKine®
SKU/Catalog Number
EK0759
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human FGF23 ELISA Kit PicoKine® (96 Tests). Quantitate Human FGF23 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human FGF23 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0759)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: Y25-I251
Sensitivity
<10 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0759 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0759 is reactive to FGF23 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK0759 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of FGF-23
Fibroblast growth factor 23 or FGF23 is a protein that in humans is encoded by the FGF23 gene. It is a member of the fibroblast growth factor (FGF) family which is responsible for phosphate metabolism. The main function of FGF23 seems to be regulation of phosphate concentration in plasma. FGF23 is secreted by Osteocytes in response to elevated Calcitriol. And it acts on the kidneys, where it decreases the expression of NPT2, a sodium-phosphate cotransporter in the proximal tubule. Thus, FGF23 decreases the reabsorption and increases excretion of phosphate. Also, FGF23 may suppress 1-alpha-hydroxylase, reducing its ability to activate vitamin D and subsequently impairing calcium absorption.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0759-CAP | Anti-Human FGF23 Pre-coated 96-well strip microplate | 1 |
| EK0759-ST | Human FGF23 Standard | 2 vials, 10 ng/tube |
| EK0759-DA | Human FGF23 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.072 | 0.109 | 0.149 | 0.210 | 0.377 | 0.656 | 1.124 | 1.963 |
Data Example Images
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Human FGF23 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human FGF23 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 40 | 254 | 441 | 43 | 245 | 473 |
| Standard deviation | 3 | 10.16 | 30.42 | 3.74 | 9.8 | 35 |
| CV (%) | 7.5% | 4% | 6.9% | 8.7% | 4% | 7.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of FGF-23 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 40 | 33 | 38 | 36 | 36 | 2.58 | 7.1% |
| Sample 2 | 254 | 252 | 258 | 248 | 253 | 3.6 | 1.4% |
| Sample 3 | 441 | 455 | 471 | 515 | 470 | 27.79 | 5.9% |
Protein Target Info & Infographic
Gene/Protein Information For FGF23 (Source: Uniprot.Org, NCBI)
Gene Name
FGF23
Full Name
Fibroblast growth factor 23
Weight
27.954kDa
Superfamily
heparin-binding growth factors family
Alternative Names
Fibroblast growth factor 23; FGF-23; Phosphatonin; Tumor-derived hypophosphatemia-inducing factor; Fibroblast growth factor 23 N-terminal peptide; Fibroblast growth factor 23 C-terminal peptide; FGF23; HYPF; UNQ3027/PRO9828 FGF23 ADHR, FGFN, HFTC2, HPDR2, HYPF, PHPTC fibroblast growth factor 23 fibroblast growth factor 23|phosphatonin|tumor-derived hypophosphatemia inducing factor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on FGF23, check out the FGF23 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for FGF23: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human FGF23 ELISA Kit PicoKine® (EK0759)
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Customer Q&As
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9 Customer Q&As for Human FGF23 ELISA Kit PicoKine®
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring FGF-23 using the EK0759 Human FGF-23 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-11-19
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2020-11-19
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2020-05-27
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-05-27
Question
Q: is it okay to use citrate plasma as samples in Human FGF-23 Picokine® ELISA Kit (Catalog # EK0759)?
Verified Customer
Verified customer
Asked: 2019-06-16
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of FGF-23 causing degradation of its protein structure. FGF-23 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the FGF-23 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-06-16
Question
Q: which procedure should I follow in order to thaw whole blood sample for FGF-23 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2019-06-04
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test FGF-23 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2019-06-04
Question
Q: if the enzyme conjugated FGF-23 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the FGF-23 antigen?
Verified Customer
Verified customer
Asked: 2019-02-26
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-02-26
Question
Q: What is the optimal O.D. value for FGF-23 ELISA kit? I performed your FGF-23 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain FGF-23 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2018-06-03
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this FGF-23 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of FGF-23 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2018-06-03
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2017-05-21
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2017-05-21
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2015-08-21
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2015-08-21
Question
Q: how do I analyze ELISA data? I measured FGF-23 level in plasma.
Verified Customer
Verified customer
Asked: 2014-08-21
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2014-08-21
