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Product Info Summary
| SKU: | A00862-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-SPARC Antibody Picoband®
SKU/Catalog Number
A00862-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SPARC Antibody Picoband® catalog # A00862-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SPARC Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00862-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SPARC recombinant protein (Position: E107-I303). Human SPARC shares 97% amino acid (aa) sequence identity with both mouse and rat SPARC.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00862-1 is reactive to SPARC in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
35 kDa
Calculated molecular weight
34632 MW
Background of SPARC
SPARC, secreted protein acidic and rich in cysteine, also known as Osteonectin is a protein that in humans is encoded by the SPARC gene. The human SPARC gene is 26.5 kb long, and contains 10 exons and 9 introns and is located on chromosome 5q31-q33. SPARC is an acidic, cysteine-rich glycoprotein consisting of a single polypeptide chain that can be broken into 4 domains: 1) an Ca++ binding domains near the glutamic acidic-rich region at the amino terminus (domain I), 2) a cysteine- rich (domain II), 3) a hydrophilic region (domain III) and 4) an EF hand motif at the carboxy terminus region (domain IV). Furthermore, SPARC is a glycoprotein in the bone that binds sodium. It is secreted by osteoblasts during bone formation, initiating mineralization and promoting mineral crystal formation. SPARC also shows affinity for collagen in addition to bone mineral calcium. A correlation between SPARC over expression and ampullary cancers and chronic pancreatitis has been found.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00862-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: human HUVEC whole cell, human placenta tissue, human A375 whole cell, human U20S whole cell, human U87 whole cell, rat testis tissue, rat kidney tissue, mouse testis tissue, mouse kidney tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of SPARC using anti-SPARC antibody (A00862-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human HUVEC whole cell lysates,
Lane 2: human placenta tissue lysates,
Lane 3: human A375 whole cell lysates,
Lane 4: human U20S whole cell lysates,
Lane 5: human U87 whole cell lysates,
Lane 6: rat testis tissue lysates,
Lane 7: rat kidney tissue lysates,
Lane 8: mouse testis tissue lysates,
Lane 9: mouse kidney tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPARC antigen affinity purified polyclonal antibody (Catalog # A00862-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPARC at approximately 35 kDa. The expected band size for SPARC is at 35 kDa.
Protein Target Info & Infographic
Gene/Protein Information For SPARC (Source: Uniprot.org, NCBI)
Gene Name
SPARC
Full Name
SPARC
Weight
34632 MW
Superfamily
SPARC family
Alternative Names
SPARC;Basement-membrane protein 40;BM-40;Osteonectin;ON;Secreted protein acidic and rich in cysteine;SPARC;ON; SPARC BM-40, OI17, ON, ONT secreted protein acidic and cysteine rich SPARC|basement-membrane protein 40|secreted protein, acidic, cysteine-rich (osteonectin)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SPARC, check out the SPARC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SPARC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SPARC Antibody Picoband® (A00862-1)
Hello CJ!
A00862-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
CD90 is upregulated in gastric cancer tissues and inhibits gastric cancer cell apoptosis by modulating the expression level of SPARC protein
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-SPARC Antibody Picoband®
Question
We are currently using anti-SPARC antibody A00862-1 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-11
Answer
The anti-SPARC antibody (A00862-1) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-11
Question
Is there a BSA free version of anti-SPARC antibody A00862-1 available?
Verified Customer
Verified customer
Asked: 2020-03-10
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-SPARC antibody A00862-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-10
Question
I am interested in using your anti-SPARC antibody for response to lipopolysaccharide studies. Has this antibody been tested with western blotting on mouse brain? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-01-01
Answer
Thank you for your inquiry. This A00862-1 anti-SPARC antibody is validated on rat brain tissue, testis tissue, mouse brain, u20s whole cell lysates, hepg2 whole cell lysates. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-01-01
Question
My colleagues were well pleased with the WB result of your anti-SPARC antibody. However we have observed positive staining in placenta extracellular space using this antibody. Is that expected? Could you tell me where is SPARC supposed to be expressed?
E. Williams
Verified customer
Asked: 2019-10-28
Answer
From what I have seen in literature, placenta does express SPARC. Generally SPARC expresses in secreted, extracellular space, extracellular. Regarding which tissues have SPARC expression, here are a few articles citing expression in various tissues:
Muscle, PNS, and Skin, Pubmed ID: 15489334
Placenta, Pubmed ID: 3410046
Boster Scientific Support
Answered: 2019-10-28
Question
Can you help my question with product A00862-1, anti-SPARC antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-09-14
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00862-1 anti-SPARC antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-09-14
Question
Is a blocking peptide available for product anti-SPARC antibody (A00862-1)?
Verified Customer
Verified customer
Asked: 2018-04-23
Answer
We do provide the blocking peptide for product anti-SPARC antibody (A00862-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-04-23
Question
Is this A00862-1 anti-SPARC antibody reactive to the isotypes of SPARC?
Verified Customer
Verified customer
Asked: 2018-03-21
Answer
The immunogen of A00862-1 anti-SPARC antibody is E.coli-derived human SPARC recombinant protein (Position: E107-I303). Human SPARC shares 97% amino acid (aa) sequence identity with both mouse and rat SPARC. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-03-21
Question
We want to test anti-SPARC antibody A00862-1 on rat pns skin for research purposes, then I may be interested in using anti-SPARC antibody A00862-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
K. Baker
Verified customer
Asked: 2018-02-05
Answer
The products we sell, including anti-SPARC antibody A00862-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-02-05
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for pns skin using anti-SPARC antibody A00862-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-12-04
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-12-04
Question
Does anti-SPARC antibody A00862-1 work for WB with pns skin?
Verified Customer
Verified customer
Asked: 2017-11-06
Answer
According to the expression profile of pns skin, SPARC is highly expressed in pns skin. So, it is likely that anti-SPARC antibody A00862-1 will work for WB with pns skin.
Boster Scientific Support
Answered: 2017-11-06
Question
We have observed staining in rat muscle. Do you have any suggestions? Is anti-SPARC antibody supposed to stain muscle positively?
Z. Walker
Verified customer
Asked: 2017-04-06
Answer
From literature muscle does express SPARC. From Uniprot.org, SPARC is expressed in tibia, placenta, muscle, pns skin, among other tissues. Regarding which tissues have SPARC expression, here are a few articles citing expression in various tissues:
Muscle, PNS, and Skin, Pubmed ID: 15489334
Placenta, Pubmed ID: 3410046
Boster Scientific Support
Answered: 2017-04-06
Question
See below the WB image, lot number and protocol we used for pns skin using anti-SPARC antibody A00862-1. Please let me know if you require anything else.
C. Gonzalez
Verified customer
Asked: 2016-12-23
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-12-23
Question
I see that the anti-SPARC antibody A00862-1 works with WB, what is the protocol used to produce the result images on the product page?
N. Jones
Verified customer
Asked: 2016-10-14
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-10-14
Question
Will A00862-1 anti-SPARC antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
B. Gonzalez
Verified customer
Asked: 2016-09-28
Answer
It shows on the product datasheet, A00862-1 anti-SPARC antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-09-28
Question
I was wanting to use your anti-SPARC antibody for WB for rat pns skin on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat pns skin identification?
B. Williams
Verified customer
Asked: 2015-02-06
Answer
You can see on the product datasheet, A00862-1 anti-SPARC antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat pns skin in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-02-06
